FastQCFastQC Report
Fri 17 Jun 2016
SRR1528055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261807
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTGTGGTAGGGACCACCCACAGAGAAGATCCAACTCACCTGCCTCTTCCA36430.2887129331189318No Hit
CCTCGTGGTAGGGGTCAACCATTTGCTCTCCCAAGCCACTGGGCCATGGCCT31780.2518610215349891No Hit
TAAAGTGGTAGGGGGTTGTGGGGACGGTCACAGATGATGTGCGGATTCTGGA31280.24789845039693073No Hit
GGCTGTGGTAGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCC29330.23244442295850318No Hit
CACAGTGGTAGGGGCCAGGCAGGATACCAGGCCCAAGGATGTTCAGAATAGT27140.21508836137380757No Hit
TAGGGTGGTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTG26890.2131070758047784No Hit
CGTGGTGGTAGGGGAACTGCCGGGCGCAGTGGCTCATGCCTGGAATCCCAGC26300.20843124186186954No Hit
GCGAGTGGTAGGGAGTGAAGGAGCAGGACTGTTACAGCCTCTGCCAAACCAG26160.2073217219432132No Hit
CGGGGTGGTAGGGTGCTTAAAAGTTAACATCTCCGGCCGGGCGCGGTGGCTC26040.2063707048700792No Hit
ACTGGTGGTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACT26000.20605369917903452No Hit
ATCTGTGGTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG24540.19448299145590411No Hit
TACCGTGGTAGGGAGGCCCTGCTGTTGGCATCCTGCTATCTGCCCCCTGCCT23690.18774662052120492No Hit
GATCGTGGTAGGGTTTGGCGGGCTGAGGTGGGAGAATCACTTAGCCCAGGAA22630.17934596970852118No Hit
CGGAGTGGTAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTCC20720.16420894796113827No Hit
GCTCGTGGTAGGGGGACTTCCCCGGGACCATGCTGCGCTGGCTGCGGGCCTT20450.16206915954658677No Hit
ACATGTGGTAGGGCTTTTTCCAGCCTCCGCCTCGGACCCTGCAGCCGCCGCG19990.15842359409957307No Hit
CCGGGTGGTAGGGTTTGGGAGGCCGAGGCGGGCAGATCACCTGAGGTTAGGA19730.15636305710778273No Hit
CTATGTGGTAGGGGTCTCTGCTTTCTACATGCTGAGAGTAAAAGTACACACC19030.150815457514501No Hit
CTTGGTGGTAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCG18750.14859641767718837No Hit
GCTGGTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT18750.14859641767718837No Hit
GGGGGTGGTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACT18510.14669438353092035No Hit
GGTGGTGGTAGGGGTTCTGCCTGTAGGGAAGCTGGAGCTCACAGGCAAAATA18400.14582261788054748No Hit
GTTGGTGGTAGGGGGGTTCGGGTGTGGGAAGCGGGAGCCCTGGCTCCCGCCG17980.14249405812457847No Hit
GAAGGTGGTAGGGACCGTGTTACTGCATGCGCCCCTGAAGCTCTGTGTTCTT17780.14090902966935515No Hit
GCCTGTGGTAGGGGAGCTGAGATGAAGTAACAAGGCTGAAGGAAGGAATTCC17410.13797672702719194No Hit
TAAGGTGGTAGGGGTTCCCTGGCTCCACCCCCCACGCCTCTTAAGTACCAAG17310.1371842127995803No Hit
GGTTGTGGTAGGGGACAACAACAGATCAAGCGAACTGCCCGGACTTCCTGTA17080.13536143007607343No Hit
TTGCGTGGTAGGGATCACTATTAGTTCTTCTGGCGCCTGCCAGAAGTAACAG16900.13393490446637243No Hit
TTCGGTGGTAGGGCGGGTGGTGGTGGTGCACGCCTTTAATCCCAGCACTCGG16890.13385565304361127No Hit
CAGAGTGGTAGGGATCTCCGCAGTTGGGCAAAATGACTTCTGCCGAGCTGGA16710.13242912743391028No Hit
TTGTGTGGTAGGGGGAACCCCCCAACGCACAATACTGGGCCCAGTTCCACAG16500.13076484755592577No Hit
TTGCGTGGTAGGGAACTATGTTCTGTTTCTTTGCTAAAGCTCTGTGTTTTCC16480.13060634471040342No Hit
TCTAGTGGTAGGGTCAACCAGAGTTTATGTGGTAGGGGCCGGTAGTGGTGGC16460.13044784186488106No Hit
GTTCGTGGTAGGGCGGTGGTAGGGGGAGTCCCAAGAGACAGGAAGAGGAAGA16420.1301308361738364No Hit
ATAGGTGGTAGGGGGTGTTCGCAGCCTTCGCCGCGCCGCTGCCGCTCTCCAA16380.12981383048279174No Hit
TCTCGTGGTAGGGGAGGCCACAGTCTGCGGTGTCTGCGGGGGTGGCTGACTG16160.12807029918204607No Hit
AGCTGTGGTAAGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG16050.12719853353167324No Hit
TGTGGTGGTAGGGGGACATACATGGTATGTACTCAATGATAAGTGGATATTA16050.12719853353167324No Hit
ACATGTGGTAGGGGTCGTGACGCCACGCTTCTGGCCTGTCGCCTCCGTCTGC15720.12458323658055472No Hit
AGGTGTGGTAGGGGGGCCCCCGTGCCAAGCAAAGACCAAAGATGCATCAGCT15640.12394922519846537No Hit
TACGGTGGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG15560.12331521381637603No Hit
GAGGGTGGTAGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATG15540.1231567109708537No Hit
GGTAGTGGTAGGGGGGAGTGTGGCGGAGCCCGCAGCTTCAGCAGCTCCGCGG15540.1231567109708537No Hit
CCGTGTGGTAGGGGCTCTTCGGTTCTCATCGCTGTGCGGACGCCAGAGCCGA15520.12299820812533137No Hit
TAATGTGGTAGGGCACTGTGACTTGATGGGAGACTGTTATCTGAGTCCACAG15340.12157168251563037No Hit
AGGGGTGGTAGGGGTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAA15330.1214924310928692No Hit
AATGGTGGTAGGGACAAGCAGCTGCGCAACCCTAGCCTCGTCCTGTTGTGAC15180.12030365975145169No Hit
GGTTGTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC15140.11998665406040701No Hit
GGGTGTGGTAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGAC15130.11990740263764586No Hit
ACTCGTGGTAGGGATGTGGATCCTAACCAACGCGCAAACATCACAAAGGGAA15100.11966964836936235No Hit
TTAAGTGGTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG15090.11959039694660119No Hit
GGGTGTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT14960.11856012845070601No Hit
ATTTGTGGTAGGGGAACCCTGCCCCTCACTCCTATTTATTAAGCATCACACT14950.11848087702794484No Hit
TGGAGTGGTAGGGAGGTGGGGGAGAGAGGAGGTTCTGAGAAAGTATTTGCTG14910.11816387133690018No Hit
CTTTGTGGTAGGGACAGCCGAACACTTAACGCCGCGCCGGAGCCACCGCACC14830.11752985995481084No Hit
CTTTGTGGTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGGCACCGACT14810.1173713571092885No Hit
ACTAGTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTGGGAGCACCCGAC14680.11634108861339333No Hit
CCCAGTGGTAGGGCTCCTCCTCCTCTGTGGTGGTTGGGGCAGCTGAGTGAGA14670.11626183719063217No Hit
TGCCGTGGTAGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGA14660.116182585767871No Hit
GGCCGTGGTAGGGGGAGCTAGAGAGATGGCTCAGTGGTTAAGAGCACTGACT14640.11602408292234867No Hit
GTGGGTGGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTG14630.11594483149958748No Hit
TTTTGTGGTAGGGGGAACACGGCTGTGCTCCCTCCTCGTCGCCCTGTACTGC14500.11491456300369234No Hit
TGGCGTGGTAGGGCGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGC14440.11443905446712534No Hit
ACATGTGGTAGGGGTTTCCTCAGTGTGTATGCCCAGCAGTGGGATTGCTGGA14430.11435980304436416No Hit
GGGCGTGGTAGGGGAGGTCGGCGAGTGCTCTGCGGTGGCCGGTGTCTGCAGG14400.11412204877608065No Hit
GACTGTGGTAGGGCTTTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGA14320.11348803739399131No Hit
CTAAGTGGTAGGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGAT14130.11198226036152914No Hit
GGACGTGGTAGGGGAAGTCCAGGCCTTACTTTCGTACACAATGCCCTATCCC14110.1118237575160068No Hit
GCCGGTGGTAGGGGAGAATTCGGTCCCGGAGCAACCTGGCGAGACGGCTCAA14090.11166525467048447No Hit
GCTTGTGGTAGGGGGGGCTGGAGAGATGGCTCAGCTGGTAAGAGCACTGACT14070.11150675182496214No Hit
GTTGGTGGTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGGCACCGACT14020.11111049471115629No Hit
TACTGTGGTAGGGTACCCTCCCGGGTGCTGCTTCTCCTTCCTGCCCACCCTA14020.11111049471115629No Hit
CTTGGTGGTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGGCACCGACT13910.11023872906078347No Hit
GGTCGTGGTAGGGGGCCGGTAGTGGTGGCGCGCGCCGGTAGGATTTGCTGAA13900.1101594776380223No Hit
GGCCGTGGTAGGGGATTTCAAAATTTAGTTACTTCTTTTTTTTTGAGATGGA13740.10889145487384362No Hit
CGCGGTGGTAGGGACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTC13680.10841594633727662No Hit
AAAAGTGGTAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGGCACCGAC13640.10809894064623196No Hit
GTATGTGGTAGGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAA13600.10778193495518727No Hit
AATGGTGGTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGGCACCGACT13580.10762343210966493No Hit
GGCTGTGGTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC13530.10722717499585911No Hit
CTATGTGGTAGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAG13510.10706867215033679No Hit
TCCTGTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT13430.10643466076824745No Hit
AGGTGTGGTAGGGATCTGGAGACCGTGGTACAGCCAAGACCCAAGCACCTCA13380.10603840365444162No Hit
TCGTGTGGTAGGGATCTTATTGACCCAGATATATTTTGATCAACGGACCAAG13280.10524588942682994No Hit
CCTCGTGGTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGGCACCGACT13270.10516663800406877No Hit
TTGGGTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT13220.10477038089026294No Hit
TACTGTGGTAGGGTGCTACCTCACTCCCTGCCTGGTGCTCAAACTCATCCTT13120.10397786666265126No Hit
TGGTGTGGTAGGGGACTTCCGGCGTGGCCATGGCGGCTAACGCGACCACGAA13090.10374011239436777No Hit
CCTTGTGGTAGGGACCGGGCGGAGCGGCCAACATGGCGGAACGCAGGCGACA13090.10374011239436777No Hit
TCCAGTGGTAGGGACCAAGGTTGATGCACCCAGCCTGAAAGGACTGATTAAT13040.10334385528056192No Hit
GACAGTGGTAGGGAGGCAATCCTCCCTAACTCATTCTGTGAAACCAGTATGC12960.1027098438984726No Hit
AGTCGTGGTAGGGGATTGAGCTCCATAGAGACAGCGCCGGGGCAAGCGCGAG12900.10223433536190558No Hit
GGCAGTGGTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACT12870.10199658109362209No Hit
TTTAGTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT12850.10183807824809975No Hit
ACTGGTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT12770.10120406686601041No Hit
TATAGTGGTAGGGAGGACAGCCAGGGCTACACAGAGAAATCCAGAGAAACCC12740.1009663125977269No Hit
GCCTGTGGTAGGGAACGCTTTCTTCAAAGGTGCTTGGTCCAATGTACTGAGA12720.10080780975220457No Hit
CAAGGTGGTAGGGTTTGGCTGGTGAGATGGCTCAGCGGATAGGAGCACTTGA12700.10064930690668224No Hit
GTCGGTGGTAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGA12690.10057005548392108No Hit
ACGAGTGGTAGGGCAGGATCTTCTGGAAACCCAAGATCCAGGGGTTCTGAAG12680.1004908040611599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGA1050.046.00000427
GCGACAC1050.046.00000428
CCGGCGT1050.046.00000419
TAACGCG1050.046.00000438
CGACAAT1050.046.00000414
CTAACGC1050.046.00000437
CCGTAGC1050.046.00000442
CTGTTGC1050.046.00000427
ACAGCGC1050.046.00000431
CCCCATA1050.046.00000418
ACGCTCA1050.046.00000414
CTCGAGC1050.046.00000418
CCGCGGA1050.046.00000439
TCCCCAT1050.046.00000417
TATACTC1050.046.00000439
GCTAACG1050.046.00000436
GCTCGAG1050.046.00000417
TTATTGC1050.046.00000421
GCCGTAG1050.046.00000441
CCCATAG1050.046.00000419
Produced by FastQC (version 0.11.5)