FastQCFastQC Report
Fri 17 Jun 2016
SRR1528054_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528054_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119364
Sequences flagged as poor quality0
Sequence length59
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTGTGGTAGGGACCACCCACAGAGAAGATCCAACTCACCTGCCTCTTCCATGAGAGA29970.2677413245378626No Hit
CCTCGTGGTAGGGGTCAACCATTTGCTCTCCCAAGCCACTGGGCCATGGCCTTGGCTGA25030.22360912089365031No Hit
TAGGGTGGTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGTCCTTCC24800.22155438266730035No Hit
TAAAGTGGTAGGGGGTTGTGGGGACGGTCACAGATGATGTGCGGATTCTGGAAGTTCCC23530.21020865420006363No Hit
GGCTGTGGTAGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCA23460.2095832990876962No Hit
ACTGGTGGTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTC23430.20931528975382452No Hit
ATCTGTGGTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAG22380.19993496306831376No Hit
CACAGTGGTAGGGGCCAGGCAGGATACCAGGCCCAAGGATGTTCAGAATAGTCTGCCTT21190.18930392615806835No Hit
GCGAGTGGTAGGGAGTGAAGGAGCAGGACTGTTACAGCCTCTGCCAAACCAGGCGCCTG20780.18564113192848797No Hit
CGTGGTGGTAGGGGAACTGCCGGGCGCAGTGGCTCATGCCTGGAATCCCAGCACTTTGA20480.1829610385897706No Hit
CGGGGTGGTAGGGTGCTTAAAAGTTAACATCTCCGGCCGGGCGCGGTGGCTCATGCCTG19980.17849421635857504No Hit
TACCGTGGTAGGGAGGCCCTGCTGTTGGCATCCTGCTATCTGCCCCCTGCCTTCCTGTC18950.16929256256231218No Hit
CTATGTGGTAGGGGTCTCTGCTTTCTACATGCTGAGAGTAAAAGTACACACCACCATGC17120.15294399319613639No Hit
GCTGGTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC16410.14660110562783865No Hit
ACATGTGGTAGGGCTTTTTCCAGCCTCCGCCTCGGACCCTGCAGCCGCCGCGATGTTGA16220.14490371317998435No Hit
TAAGGTGGTAGGGGTTCCCTGGCTCCACCCCCCACGCCTCTTAAGTACCAAGCAACCAT15840.1415089282842757No Hit
GCTCGTGGTAGGGGGACTTCCCCGGGACCATGCTGCGCTGGCTGCGGGCCTTTGTGCTG15550.13891817139018228No Hit
TTGCGTGGTAGGGATCACTATTAGTTCTTCTGGCGCCTGCCAGAAGTAACAGAAATCAG15430.13784613405469534No Hit
GAAGGTGGTAGGGACCGTGTTACTGCATGCGCCCCTGAAGCTCTGTGTTCTTAGGAGAG15270.13641675094071276No Hit
GGGGGTGGTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTC14990.13391533049124324No Hit
CGGAGTGGTAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTCCTGAGTGC14540.12989519048316722No Hit
CAGAGTGGTAGGGATCTCCGCAGTTGGGCAAAATGACTTCTGCCGAGCTGGACACCCCT14290.12766177936756945No Hit
TTGCGTGGTAGGGAACTATGTTCTGTTTCTTTGCTAAAGCTCTGTGTTTTCCATCTGCT14000.125071022473476No Hit
CTTGGTGGTAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGC13970.12480301313960428No Hit
CCCAGTGGTAGGGCTCCTCCTCCTCTGTGGTGGTTGGGGCAGCTGAGTGAGACTGTAAA13550.1210508824654No Hit
TTCGGTGGTAGGGCGGGTGGTGGTGGTGCACGCCTTTAATCCCAGCACTCGGGAGGCAG13330.11908548068367394No Hit
GTTGGTGGTAGGGGGGTTCGGGTGTGGGAAGCGGGAGCCCTGGCTCCCGCCGGCGCGGG13330.11908548068367394No Hit
TCTAGTGGTAGGGTCAACCAGAGTTTATGTGGTAGGGGCCGGTAGTGGTGGCGCACGCC13300.11881747134980221No Hit
GCCTGTGGTAGGGGAGCTGAGATGAAGTAACAAGGCTGAAGGAAGGAATTCCCCCCGTG13170.11765609756969135No Hit
GGTGGTGGTAGGGGTTCTGCCTGTAGGGAAGCTGGAGCTCACAGGCAAAATAAGGAAGC13090.11694140601270007No Hit
TGTGGTGGTAGGGGGACATACATGGTATGTACTCAATGATAAGTGGATATTAGCCCCAA13050.11658406023420444No Hit
ATAGGTGGTAGGGGGTGTTCGCAGCCTTCGCCGCGCCGCTGCCGCTCTCCAACGCCAGC12990.11604804156646095No Hit
TTAAGTGGTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAG12950.1156906957879653No Hit
AGCTGTGGTAAGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAG12870.11497600423097402No Hit
ACATGTGGTAGGGGTCGTGACGCCACGCTTCTGGCCTGTCGCCTCCGTCTGCCAAATGC12780.1141719762293588No Hit
CTAAGTGGTAGGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCTGAGAG12740.11381463045086317No Hit
GAGGGTGGTAGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTA12710.11354662111699143No Hit
GCCGGTGGTAGGGGAGAATTCGGTCCCGGAGCAACCTGGCGAGACGGCTCAACGGCGCT12690.11336794822774361No Hit
GGGCGTGGTAGGGGAGGTCGGCGAGTGCTCTGCGGTGGCCGGTGTCTGCAGGCCCAGCT12670.11318927533849579No Hit
GGCCGTGGTAGGGGGAGCTAGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTC12660.11309993889387189No Hit
TCTCGTGGTAGGGGAGGCCACAGTCTGCGGTGTCTGCGGGGGTGGCTGACTGGCCTGAG12550.11211723800300885No Hit
ACTCGTGGTAGGGATGTGGATCCTAACCAACGCGCAAACATCACAAAGGGAAAGGAGAG12490.11158121933526538No Hit
GGACGTGGTAGGGGAAGTCCAGGCCTTACTTTCGTACACAATGCCCTATCCCTAAAAGT12280.10970515399816325No Hit
TTGTGTGGTAGGGGGAACCCCCCAACGCACAATACTGGGCCCAGTTCCACAGTGTGAGG12240.10934780821966761No Hit
ATTTGTGGTAGGGGAACCCTGCCCCTCACTCCTATTTATTAAGCATCACACTACCAGGG12210.10907979888579586No Hit
GACAGTGGTAGGGAGGCAATCCTCCCTAACTCATTCTGTGAAACCAGTATGCCCTGATA12150.10854378021805239No Hit
GGTTGTGGTAGGGGACAACAACAGATCAAGCGAACTGCCCGGACTTCCTGTAAAGGGGG12140.10845444377342849No Hit
AATGGTGGTAGGGACAAGCAGCTGCGCAACCCTAGCCTCGTCCTGTTGTGACCAGCTGC12140.10845444377342849No Hit
TACTGTGGTAGGGTGCTACCTCACTCCCTGCCTGGTGCTCAAACTCATCCTTCTTCCTG12130.10836510732880457No Hit
TGGCGTGGTAGGGCGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCTG12120.10827577088418067No Hit
TACGGTGGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAG11920.10648904199170244No Hit
GTTGGTGGTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGGCACCGACTGCTCTTA11910.10639970554707852No Hit
ACATGTGGTAGGGGTTTCCTCAGTGTGTATGCCCAGCAGTGGGATTGCTGGATCATAAG11880.10613169621320678No Hit
TAATGTGGTAGGGCACTGTGACTTGATGGGAGACTGTTATCTGAGTCCACAGTACCCAG11850.10586368687933506No Hit
GGGTGTGGTAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTT11830.10568501399008724No Hit
GACTGTGGTAGGGCTTTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACGCCAGA11830.10568501399008724No Hit
CTTTGTGGTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGGCACCGACTGCTCTTA11680.10434496732072857No Hit
ACTAGTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTGGGAGCACCCGACTGCTCTT11680.10434496732072857No Hit
GTTCGTGGTAGGGCGGTGGTAGGGGGAGTCCCAAGAGACAGGAAGAGGAAGATGACCTG11670.10425563087610465No Hit
AATGGTGGTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGGCACCGACTGCTCTTA11470.10246890198362642No Hit
CCTCGTGGTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGGCACCGACTGCTCTTA11390.10175421042663511No Hit
ATATGTGGTAGGGGGGCTGGTGAGACGGCTCAGTGGTTAAGAGCACTGACTACTCTTCC11380.10166487398201121No Hit
AGGTGTGGTAGGGATCTGGAGACCGTGGTACAGCCAAGACCCAAGCACCTCACTCTCCA11300.10095018242501992No Hit
GCCTGTGGTAGGGAACGCTTTCTTCAAAGGTGCTTGGTCCAATGTACTGAGAGGCATGG11260.10059283664652428No Hit
AGGTGTGGTAGGGGGGCCCCCGTGCCAAGCAAAGACCAAAGATGCATCAGCTTAAAGAG11230.10032482731265255No Hit
ACTGGTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC11200.1000568179787808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG550.053.00000416
GTCGAAC1100.053.00000439
TTTACCG550.053.00000419
ACGTTCG550.053.00000442
TAGACAG550.053.00000419
GACAATT550.053.00000425
AGCCATA550.053.00000415
ACTCTCG1100.053.00000418
CGCGCCT550.053.00000443
TTATAGG1100.053.00000450
TTGAGCC550.053.00000439
TAGCACG550.053.00000422
GCCGATT550.053.00000439
ACCTAGA550.053.00000444
CTAGTTC1100.053.00000423
ACGGCCC550.053.00000446
TTAAGCC550.053.00000447
TACGAGA3500.053.00000439
GTACTCC550.053.00000449
CCCCATG1100.053.00000415
Produced by FastQC (version 0.11.5)