FastQCFastQC Report
Fri 17 Jun 2016
SRR1528053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16462336
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAC301.8629253E-646.042
CGACTAA301.8629253E-646.025
CGCATGA844700.045.1096233
TTCATGA2454500.045.0217133
ATGGGGC2538300.044.9371229
TCATGAT6442700.044.9336624
TTCGCAT102400.044.9218751
TACGCAT67750.044.9136541
TGATGGG16555950.044.902237
CATGATG16795900.044.897785
CGGCATG194300.044.8636172
ATGATGG16754000.044.855496
CACATGA809350.044.854763
GATGGGG10959750.044.8506058
TTTCATG927150.044.819182
GTCATGA1443800.044.728773
ATGGGAG1594450.044.726279
GCATGAT4430200.044.652254
ATGGGGA3168750.044.652129
ACATGAT3716400.044.643424
Produced by FastQC (version 0.11.5)