##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528053_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16462336
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48194436075172	31.0	31.0	34.0	30.0	34.0
2	31.966155350006222	31.0	31.0	34.0	30.0	34.0
3	32.12385581244363	33.0	31.0	34.0	30.0	34.0
4	35.78615914533636	37.0	35.0	37.0	35.0	37.0
5	35.73737141557553	37.0	35.0	37.0	35.0	37.0
6	35.82000494947983	37.0	35.0	37.0	35.0	37.0
7	35.8764406825374	37.0	35.0	37.0	35.0	37.0
8	35.834334811292884	37.0	35.0	37.0	33.0	37.0
9	37.00904549633782	39.0	37.0	39.0	33.0	39.0
10	37.49953597107968	39.0	37.0	39.0	35.0	39.0
11	37.663451711834824	39.0	37.0	39.0	35.0	39.0
12	37.502045639209406	39.0	37.0	39.0	35.0	39.0
13	37.459396770907844	39.0	37.0	39.0	35.0	39.0
14	38.11395885735779	40.0	38.0	40.0	34.0	40.0
15	37.382772590718595	39.0	37.0	40.0	32.0	40.0
16	36.98051121055967	39.0	36.0	40.0	32.0	40.0
17	36.82454179042391	39.0	36.0	40.0	31.0	40.0
18	36.95566583017137	39.0	36.0	40.0	32.0	40.0
19	37.05475510887398	39.0	36.0	40.0	32.0	40.0
20	37.162210454215	39.0	36.0	40.0	32.0	40.0
21	37.018852913705565	39.0	36.0	40.0	31.0	40.0
22	37.10705558433506	39.0	36.0	40.0	32.0	40.0
23	37.1194600814854	39.0	36.0	40.0	32.0	40.0
24	37.063274798910676	39.0	36.0	40.0	32.0	40.0
25	37.15232759190433	39.0	36.0	40.0	32.0	40.0
26	37.037545765072466	39.0	36.0	40.0	32.0	40.0
27	36.90465715193761	39.0	35.0	40.0	31.0	40.0
28	36.89045588669798	39.0	35.0	40.0	31.0	40.0
29	36.96774528232202	39.0	35.0	40.0	31.0	40.0
30	36.947969231098185	39.0	35.0	40.0	31.0	40.0
31	36.94251307955323	39.0	35.0	40.0	31.0	40.0
32	36.907549208083225	39.0	35.0	40.0	31.0	40.0
33	36.817189310192674	39.0	35.0	40.0	31.0	40.0
34	36.73205534135617	39.0	35.0	40.0	31.0	40.0
35	36.696964148951885	39.0	35.0	40.0	31.0	40.0
36	36.68142850443582	39.0	35.0	40.0	31.0	40.0
37	36.561358485211336	39.0	35.0	40.0	31.0	40.0
38	36.3426905513288	39.0	35.0	40.0	31.0	40.0
39	36.22221008002752	38.0	35.0	40.0	30.0	40.0
40	36.20889173930115	38.0	35.0	40.0	30.0	40.0
41	36.11958393997061	38.0	35.0	40.0	30.0	40.0
42	36.02299588588156	38.0	35.0	40.0	30.0	40.0
43	35.84261146170264	38.0	35.0	40.0	30.0	40.0
44	35.64750136311153	38.0	34.0	40.0	30.0	40.0
45	35.53077564447719	37.0	34.0	40.0	29.0	40.0
46	35.423522093097844	37.0	34.0	40.0	29.0	40.0
47	35.18245108106164	37.0	34.0	40.0	29.0	40.0
48	35.06197437593304	37.0	34.0	40.0	28.0	40.0
49	34.99304940683995	36.0	34.0	40.0	28.0	40.0
50	34.89562331858613	36.0	34.0	39.0	28.0	40.0
51	34.74322009950471	36.0	34.0	39.0	28.0	40.0
52	33.790260628868225	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	5.0
13	12.0
14	28.0
15	84.0
16	273.0
17	787.0
18	2040.0
19	4409.0
20	8578.0
21	15440.0
22	25550.0
23	39202.0
24	58456.0
25	83977.0
26	117249.0
27	158677.0
28	211092.0
29	277488.0
30	357315.0
31	453992.0
32	568265.0
33	726953.0
34	1047155.0
35	1646759.0
36	1619936.0
37	2698768.0
38	4862070.0
39	1477773.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.207532150965697	20.202406268466394	34.844696402746244	18.745365177821665
2	22.45536720912512	22.51438677961621	36.19004010123472	18.840205910023947
3	24.119985158849875	21.322891234876995	36.42810473556122	18.129018870711906
4	26.040982276148416	21.846042991711503	38.30930798642429	13.803666745715795
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.57956756562373	0.3261444791310298	0.04285540035144465	0.051432554893789076
12	99.59649711924237	0.2766739787111623	0.08920362213479302	0.03762527991167232
13	98.78031890492333	0.6309189655708644	0.2043695378347277	0.38439259167107265
14	65.65264492232451	22.248416020666813	4.210738986253227	7.888200070755451
15	35.48749096118558	26.21030818469505	16.331369982972042	21.970830871147324
16	30.597735339626166	17.383772266584767	26.05330130547694	25.965191088312135
17	24.97734221923304	17.198027060072153	29.1979157757441	28.626714944950706
18	25.32779673553012	16.979309619242372	31.356673803766366	26.33621984146114
19	26.20490190456567	17.4694769928156	28.11334916259758	28.21227194002115
20	29.680836304155132	17.1999040719373	24.407471697819798	28.711787926087766
21	31.09490050500731	18.459123905623116	21.284470199126055	29.161505390243526
22	29.914448350464962	20.548189515752806	21.42494843988119	28.112413693901033
23	29.62879022758374	20.70813036497372	20.32993373479924	29.3331456726433
24	29.54612273738065	22.874937068469507	20.800213286862814	26.77872690728703
25	32.11477399076291	19.835514230787172	22.312926913896057	25.73678486455385
26	31.10223239277828	18.756663695844868	22.506453519111748	27.634650392265108
27	31.745312451404224	20.11178121987062	21.11109261771841	27.03181371100675
28	30.694574573134698	19.580161648990764	21.107854924112836	28.6174088537617
29	29.32577126356794	19.619378440580974	21.555646780627	29.49920351522409
30	28.915118729201005	20.383607769881504	21.582896862267905	29.11837663864958
31	29.013300421033804	19.516592298930117	21.163533534973407	30.30657374506267
32	29.68948027789009	21.433294764485428	20.218127002145987	28.65909795547849
33	30.083306524663328	22.043463333514758	20.188793376590052	27.68443676523186
34	29.064216645802883	21.90770495754673	21.831974514431003	27.19610388221939
35	31.76704691241875	22.321959653842566	18.907541432758997	27.00345200097969
36	34.65550089610612	21.489356067085495	19.536461897023607	24.31868113978478
37	33.61210705455168	21.238298137032313	20.474822042266663	24.674772766149346
38	30.53394730857152	22.9599918261904	23.713779138027554	22.792281727210526
39	30.063856065141664	24.857632598435604	23.193804330078063	21.884707006344666
40	31.010477492380183	25.070269492737847	19.323515204646533	24.595737810235438
41	30.45932849384194	25.504812925699	18.93746428210431	25.09839429835474
42	32.60986776117314	23.867845972770816	17.949129455260785	25.57315681079526
43	32.451160029779494	22.426446647668957	18.443342427222966	26.679050895328587
44	30.051202939850093	22.444414936009082	19.587044025829627	27.917338098311202
45	27.572490319721332	22.686373306923148	20.895509604469257	28.845626768886262
46	27.550184858333594	19.800865442182687	22.77026176600939	29.878687933474325
47	26.92240032034336	18.409361830544587	24.627677384303176	30.04056046480888
48	27.511812418359092	18.850325980468384	23.372430255341648	30.265431345830873
49	28.600424629894565	19.538059483174198	22.951985672021273	28.909530214909964
50	27.074098110984977	22.207273621435014	22.367111204630984	28.35151706294903
51	27.888952090395918	21.807409349438622	22.39040073049171	27.913237829673747
52	28.888482169237705	20.89346250738656	23.634580171368146	26.58347515200759
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	5.0
6	8.0
7	10.5
8	13.0
9	605.0
10	1197.0
11	1955.5
12	2714.0
13	4858.5
14	9582.5
15	12162.0
16	12204.0
17	12246.0
18	12515.5
19	12785.0
20	15102.0
21	17419.0
22	23464.5
23	29510.0
24	36692.0
25	43874.0
26	54797.0
27	65720.0
28	70005.5
29	74291.0
30	75671.0
31	77051.0
32	93218.5
33	109386.0
34	117540.5
35	125695.0
36	146152.5
37	166610.0
38	185110.0
39	231979.5
40	260349.0
41	284350.0
42	308351.0
43	368551.0
44	428751.0
45	454134.5
46	479518.0
47	550133.5
48	620749.0
49	685168.0
50	749587.0
51	827970.5
52	906354.0
53	1062776.0
54	1219198.0
55	1414693.0
56	1610188.0
57	1771618.5
58	1933049.0
59	1907484.5
60	1881920.0
61	1728137.5
62	1574355.0
63	1382566.0
64	1002883.0
65	814989.0
66	695769.0
67	576549.0
68	493901.5
69	411254.0
70	339364.0
71	267474.0
72	215269.5
73	163065.0
74	114829.5
75	66594.0
76	43319.5
77	20045.0
78	17113.5
79	14182.0
80	8579.5
81	2977.0
82	1704.5
83	432.0
84	325.5
85	219.0
86	159.5
87	100.0
88	57.0
89	7.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.6462336E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.897894503386119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.92307649605529	3.6696532454680693
2	8.723173564140831	0.8545036770377696
3	3.669595650013767	0.5391987709155612
4	2.236637801031122	0.438192639669438
5	1.661491508637589	0.4068905063789379
6	1.1809823894250928	0.347059629225658
7	0.8963424195383854	0.3073133426866211
8	0.6829060291213466	0.2675841349090147
9	0.521499045287826	0.22988225766927167
>10	2.42967609923145	2.1308057407839995
>50	0.2891049257278371	1.0177590574326993
>100	1.2196071393679522	18.059008334322872
>500	1.0816347689388102	37.772379837072954
>1k	0.4838892387357827	33.84264854105487
>5k	3.829247470871401E-4	0.11712028537229396
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.006724440565421578	0.0	0.0	0.0	0.0
12	0.01211249727863652	0.0	0.0	0.0	0.0
13	0.01972988523621435	0.0	0.0	0.0	0.0
14	0.04764208433116661	0.0	0.0	0.0	0.0
15	0.06627856459739372	0.0	0.0	0.0	0.0
16	0.07944194554162909	0.0	0.0	0.0	0.0
17	0.08823778107797095	0.0	0.0	0.0	0.0
18	0.09893492636767953	0.0	0.0	0.0	0.0
19	0.1089213584268964	0.0	0.0	0.0	0.0
20	0.12100955781731099	0.0	0.0	0.0	0.0
21	0.13354119366777595	0.0	0.0	0.0	0.0
22	0.1458845208845209	0.0	0.0	0.0	0.0
23	0.15782693294560382	0.0	0.0	0.0	0.0
24	0.16955066401268934	0.0	0.0	0.0	0.0
25	0.18318177930519702	0.0	0.0	0.0	0.0
26	0.19764509726930612	0.0	0.0	0.0	0.0
27	0.21167712771747582	0.0	0.0	0.0	0.0
28	0.22532646642614998	0.0	0.0	0.0	0.0
29	0.242596190479893	0.0	0.0	0.0	0.0
30	0.26360778931981466	0.0	0.0	0.0	0.0
31	0.2804037045532299	0.0	0.0	0.0	0.0
32	0.29634311922682177	0.0	0.0	0.0	0.0
33	0.3088504571890648	0.0	0.0	0.0	0.0
34	0.3215886250894162	0.0	0.0	0.0	0.0
35	0.3349281657232607	0.0	0.0	0.0	0.0
36	0.34824340846888313	0.0	0.0	0.0	0.0
37	0.36004610767268996	0.0	0.0	0.0	0.0
38	0.37272353085248655	0.0	0.0	0.0	0.0
39	0.38705321043137497	0.0	0.0	0.0	0.0
40	0.40358792336640437	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAC	30	1.8629253E-6	46.0	42
CGACTAA	30	1.8629253E-6	46.0	25
CGCATGA	84470	0.0	45.109623	3
TTCATGA	245450	0.0	45.021713	3
ATGGGGC	253830	0.0	44.937122	9
TCATGAT	644270	0.0	44.933662	4
TTCGCAT	10240	0.0	44.921875	1
TACGCAT	6775	0.0	44.913654	1
TGATGGG	1655595	0.0	44.90223	7
CATGATG	1679590	0.0	44.89778	5
CGGCATG	19430	0.0	44.863617	2
ATGATGG	1675400	0.0	44.85549	6
CACATGA	80935	0.0	44.85476	3
GATGGGG	1095975	0.0	44.850605	8
TTTCATG	92715	0.0	44.81918	2
GTCATGA	144380	0.0	44.72877	3
ATGGGAG	159445	0.0	44.72627	9
GCATGAT	443020	0.0	44.65225	4
ATGGGGA	316875	0.0	44.65212	9
ACATGAT	371640	0.0	44.64342	4
>>END_MODULE