FastQCFastQC Report
Fri 17 Jun 2016
SRR1528052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16313084
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAA453.110472E-1046.045
CGCATGA839000.045.1940423
TTCATGA2449050.045.080583
TACGCAT67050.045.0395241
ATGGGGC2519400.045.011319
TCATGAT6404350.044.9585154
TGATGGG16406150.044.918287
CATGATG16636100.044.9148455
ATGATGG16599000.044.879586
GATGGGG10850750.044.865348
CACATGA795850.044.841113
ATGGGAG1573100.044.8084079
TTTCATG938500.044.7697372
TTCGCAT103550.044.7339441
GTCATGA1443700.044.711163
TACATGA1342550.044.699713
ACATGAT3660450.044.68744
ATGGGGA3149300.044.6649749
GCATGAT4372050.044.62174
ATGGGAC696750.044.564059
Produced by FastQC (version 0.11.5)