##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528052_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16313084
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.506745076528755	31.0	31.0	34.0	30.0	34.0
2	31.987806781354156	31.0	31.0	34.0	30.0	34.0
3	32.1449776755885	33.0	31.0	34.0	30.0	34.0
4	35.81781280596606	37.0	35.0	37.0	35.0	37.0
5	35.85365612044908	37.0	35.0	37.0	35.0	37.0
6	35.863243516676555	37.0	35.0	37.0	35.0	37.0
7	35.86685154076323	37.0	35.0	37.0	35.0	37.0
8	35.836556042989784	37.0	35.0	37.0	33.0	37.0
9	36.988047569668616	39.0	37.0	39.0	33.0	39.0
10	37.48029128029991	39.0	37.0	39.0	35.0	39.0
11	37.652302532126974	39.0	37.0	39.0	35.0	39.0
12	37.518801778989186	39.0	37.0	39.0	35.0	39.0
13	37.45280395785371	39.0	37.0	39.0	35.0	39.0
14	38.05386063113511	40.0	38.0	40.0	33.0	40.0
15	37.374107311652416	39.0	37.0	40.0	32.0	40.0
16	36.94615267107066	39.0	36.0	40.0	32.0	40.0
17	36.80204245867918	39.0	36.0	40.0	31.0	40.0
18	36.85922275640829	39.0	36.0	40.0	31.0	40.0
19	37.0283427707477	39.0	36.0	40.0	32.0	40.0
20	37.141910199199614	39.0	36.0	40.0	32.0	40.0
21	37.030060778207236	39.0	36.0	40.0	32.0	40.0
22	37.0967055646866	39.0	36.0	40.0	32.0	40.0
23	37.20546347949903	39.0	36.0	40.0	32.0	40.0
24	37.184316466463365	39.0	36.0	40.0	32.0	40.0
25	37.182148452125915	39.0	36.0	40.0	32.0	40.0
26	36.93892644701639	39.0	35.0	40.0	31.0	40.0
27	36.783286777656514	39.0	35.0	40.0	31.0	40.0
28	36.80131580270169	39.0	35.0	40.0	31.0	40.0
29	36.87087965708998	39.0	35.0	40.0	31.0	40.0
30	36.74254929356092	39.0	35.0	40.0	31.0	40.0
31	36.78231068999583	39.0	35.0	40.0	31.0	40.0
32	36.82998236262377	39.0	35.0	40.0	31.0	40.0
33	36.84680793650054	39.0	35.0	40.0	31.0	40.0
34	36.796727890324114	39.0	35.0	40.0	31.0	40.0
35	36.58513289087459	39.0	35.0	40.0	31.0	40.0
36	36.62126830218002	39.0	35.0	40.0	31.0	40.0
37	36.51096279526299	39.0	35.0	40.0	31.0	40.0
38	36.34843975547481	38.0	35.0	40.0	31.0	40.0
39	36.23137268219792	38.0	35.0	40.0	30.0	40.0
40	36.16153573413831	38.0	35.0	40.0	30.0	40.0
41	36.04845141482751	38.0	35.0	40.0	30.0	40.0
42	35.94464756020382	38.0	35.0	40.0	30.0	40.0
43	35.80007808456084	38.0	35.0	40.0	30.0	40.0
44	35.51011372221218	37.0	34.0	40.0	29.0	40.0
45	35.39009037163053	37.0	34.0	40.0	29.0	40.0
46	35.28143826145933	37.0	34.0	40.0	29.0	40.0
47	35.173762974554656	37.0	34.0	40.0	29.0	40.0
48	35.05280184911694	36.0	34.0	40.0	29.0	40.0
49	34.792096761102925	36.0	34.0	39.0	28.0	40.0
50	34.694772367996144	36.0	33.0	39.0	28.0	40.0
51	34.539740186466275	35.0	33.0	39.0	27.0	40.0
52	33.161290470888275	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	10.0
14	29.0
15	89.0
16	237.0
17	727.0
18	1997.0
19	4449.0
20	8543.0
21	15338.0
22	25090.0
23	39651.0
24	59618.0
25	85608.0
26	118508.0
27	161598.0
28	215573.0
29	281627.0
30	363574.0
31	458247.0
32	572340.0
33	734742.0
34	1060821.0
35	1627619.0
36	1630817.0
37	2723313.0
38	4847251.0
39	1275663.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.237141916267948	20.175896844520633	34.83958643258381	18.74737480662761
2	22.46721098230108	22.499234356912527	36.183709959441146	18.84984470134525
3	24.120497387250627	21.286054801164514	36.440270889305786	18.153176922279073
4	25.987556981868053	21.82563395125042	38.356438304369675	13.83037076251186
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.58536350330814	0.3199395037750066	0.04320458351100258	0.051492409405848705
12	99.63975542576745	0.2502592397611635	0.08068983154871268	0.029295502922684638
13	98.8005701435731	0.6211700987992216	0.2011452892659659	0.377114468361715
14	65.71361368579969	22.199953117387246	4.203074047801139	7.8833591490119215
15	35.53207351841013	26.237417768461192	16.196808647586195	22.03370006554248
16	30.60624220411052	17.355032316390943	26.05230868669591	25.986416792802636
17	24.97915170423937	17.178444002372576	29.20480272154548	28.637601571842573
18	25.327031970165788	16.960336868246372	31.34182966261928	26.37080149896856
19	26.237552629533447	17.44807419614832	28.096244707622418	28.218128466695813
20	29.673230395920232	17.16848267317204	24.402222167188008	28.756064763719724
21	31.10009119060504	18.444746560490955	21.240189776500877	29.214972472403133
22	29.91281109077842	20.564505154267582	21.43683560999257	28.085848144961428
23	29.579679722117536	20.72439521552148	20.32990205898529	29.366023003375695
24	29.616153512113346	22.875827771131444	20.71875557068179	26.789263146073424
25	32.12689887454757	19.829475530194046	22.29473593098644	25.74888966427194
26	31.0528346448777	18.77088354354088	22.501042721290467	27.675239090290958
27	31.71891961078604	20.141231418902766	21.110110142263718	27.029738828047474
28	30.721137707621686	19.5505215322866	21.107137068625406	28.621203691466313
29	29.335660871972465	19.58323760240553	21.558946180869295	29.522155344752715
30	28.868115924616095	20.3730759922526	21.672560504194056	29.086247578937254
31	29.010688598182906	19.514568796433586	21.17172939218605	30.303013213197456
32	29.672985193970682	21.419886025229808	20.22222162283968	28.68490715795983
33	30.11398090023934	22.025271248526643	20.131049407947632	27.72969844328638
34	29.093989830494344	21.927368240119407	21.7664973710673	27.212144558318958
35	31.580582800897734	22.346798434925	19.110978647569034	26.961640116608237
36	34.67766119514863	21.487776315011924	19.503620529386104	24.33094196045334
37	33.628552393894374	21.249409369804017	20.448389771057393	24.673648465244217
38	30.568033610321628	22.987750201004296	23.673359372145697	22.770856816528376
39	30.130299089982003	24.84802383166788	23.10609079190667	21.915586286443446
40	31.017874976920368	25.059823145641868	19.32774330102144	24.59455857641633
41	30.469394996065734	25.502492355216216	18.923166214309937	25.10494643440811
42	32.6087390955628	23.861484437890468	17.92142429966032	25.608352166886412
43	32.492108788258555	22.39456377469766	18.44982224084667	26.663505196197114
44	30.0475556921058	22.424251600739627	19.596024884074648	27.93216782307993
45	27.54772794647536	22.682467643763744	20.911839845856246	28.857964563904652
46	27.552932357854594	19.77427444130123	22.77394023104399	29.898852969800195
47	26.91674976969407	18.41595985161359	24.600982867494583	30.06630751119776
48	27.54054965940223	18.797169192532817	23.376756963919266	30.285524184145686
49	28.51668636046992	19.51831425621299	23.086529806381183	28.87846957693591
50	27.0667214120886	22.19547818180793	22.359119832889967	28.3786805732135
51	27.906672950375295	21.781571160915984	22.398701557596347	27.913054331112374
52	28.824892950958876	20.895822028501783	23.67409497799435	26.60519004254499
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	5.0
6	8.0
7	11.5
8	15.0
9	541.5
10	1068.0
11	1819.5
12	2571.0
13	4777.5
14	9530.0
15	12076.0
16	12346.5
17	12617.0
18	12679.0
19	12741.0
20	15026.5
21	17312.0
22	23347.5
23	29383.0
24	36544.5
25	43706.0
26	54569.0
27	65432.0
28	69730.0
29	74028.0
30	75064.0
31	76100.0
32	91845.0
33	107590.0
34	115697.5
35	123805.0
36	144286.0
37	164767.0
38	182782.5
39	229389.0
40	257980.0
41	280898.5
42	303817.0
43	363641.5
44	423466.0
45	448914.0
46	474362.0
47	543607.5
48	612853.0
49	676676.5
50	740500.0
51	818420.0
52	896340.0
53	1053011.5
54	1209683.0
55	1403512.5
56	1597342.0
57	1755155.0
58	1912968.0
59	1890262.0
60	1867556.0
61	1714740.5
62	1561925.0
63	1371806.5
64	995332.0
65	808976.0
66	690852.5
67	572729.0
68	491106.5
69	409484.0
70	337140.0
71	264796.0
72	213440.0
73	162084.0
74	113861.5
75	65639.0
76	42934.0
77	20229.0
78	17215.5
79	14202.0
80	8503.0
81	2804.0
82	1587.5
83	371.0
84	284.0
85	197.0
86	136.5
87	76.0
88	45.0
89	7.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.6313084E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.892718663758807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.75706386257175	3.6576528160821393
2	8.74561385632973	0.8557965628178421
3	3.7117765714169866	0.544820355200237
4	2.29013400866998	0.4481992562691354
5	1.6383246834548935	0.4007930878018248
6	1.1847816718659143	0.34780820390506334
7	0.9063126813118012	0.31040330797388765
8	0.6792722491361463	0.2658790408897477
9	0.4951142420553739	0.21802092235174114
>10	2.4679058436244223	2.1576191328575693
>50	0.29856567038744053	1.0452447142558599
>100	1.2586885615751144	18.589077221150045
>500	1.0932312776532171	38.07237755840322
>1k	0.4728257696706555	32.97071378718504
>5k	3.890502767449167E-4	0.11559403285668014
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.00709246639078178	0.0	0.0	0.0	0.0
12	0.012542079719567435	0.0	0.0	0.0	0.0
13	0.019591635769177673	0.0	0.0	0.0	0.0
14	0.047244285629866184	0.0	0.0	0.0	0.0
15	0.06538922989668906	0.0	0.0	0.0	0.0
16	0.07818877166328574	0.0	0.0	0.0	0.0
17	0.08689344087236969	0.0	0.0	0.0	0.0
18	0.09749842519047901	0.0	0.0	0.0	0.0
19	0.1073248933187618	0.0	0.0	0.0	0.0
20	0.11855514260822785	0.0	0.0	0.0	0.0
21	0.13041678691778943	0.0	0.0	0.0	0.0
22	0.14270140459032762	0.0	0.0	0.0	0.0
23	0.15525574440737264	0.0	0.0	0.0	0.0
24	0.1676323128109927	0.0	0.0	0.0	0.0
25	0.18151687320435547	0.0	0.0	0.0	0.0
26	0.1956466355472699	0.0	0.0	0.0	0.0
27	0.20949441564819993	0.0	0.0	0.0	0.0
28	0.22328089526174205	0.0	0.0	0.0	0.0
29	0.24081283465468578	0.0	0.0	0.0	0.0
30	0.2618695520724346	0.0	0.0	0.0	0.0
31	0.2788375269814095	0.0	0.0	0.0	0.0
32	0.2943404202418133	0.0	0.0	0.0	0.0
33	0.30663729801182904	0.0	0.0	0.0	0.0
34	0.3191732476826577	0.0	0.0	0.0	0.0
35	0.3322363815450224	0.0	0.0	0.0	0.0
36	0.34534242574855867	0.0	0.0	0.0	0.0
37	0.3565726750380247	0.0	0.0	0.0	0.0
38	0.3692741360248007	0.0	0.0	0.0	0.0
39	0.3832077368080738	0.0	0.0	0.0	0.0
40	0.40021249200948145	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAA	45	3.110472E-10	46.0	45
CGCATGA	83900	0.0	45.194042	3
TTCATGA	244905	0.0	45.08058	3
TACGCAT	6705	0.0	45.039524	1
ATGGGGC	251940	0.0	45.01131	9
TCATGAT	640435	0.0	44.958515	4
TGATGGG	1640615	0.0	44.91828	7
CATGATG	1663610	0.0	44.914845	5
ATGATGG	1659900	0.0	44.87958	6
GATGGGG	1085075	0.0	44.86534	8
CACATGA	79585	0.0	44.84111	3
ATGGGAG	157310	0.0	44.808407	9
TTTCATG	93850	0.0	44.769737	2
TTCGCAT	10355	0.0	44.733944	1
GTCATGA	144370	0.0	44.71116	3
TACATGA	134255	0.0	44.69971	3
ACATGAT	366045	0.0	44.6874	4
ATGGGGA	314930	0.0	44.664974	9
GCATGAT	437205	0.0	44.6217	4
ATGGGAC	69675	0.0	44.56405	9
>>END_MODULE