FastQCFastQC Report
Fri 17 Jun 2016
SRR1528051_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528051_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14671238
Sequences flagged as poor quality0
Sequence length59
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATGA750700.051.990413
TTCATGA2167000.051.7807773
ATGGGGC2211600.051.777819
TGATGGG14756200.051.6680157
TCATGAT5727300.051.6623424
CATGATG14983250.051.653185
GATGGGG9707400.051.637258
TTCGCAT88700.051.62571
ATGATGG14947450.051.5999576
CACATGA729500.051.4924623
TTTCATG817400.051.379012
GCATGAT3954350.051.3279764
ATGGGAG1411350.051.325159
ATGGGGA2819600.051.3063939
ACATGAT3337700.051.2977454
TACGCAT62000.051.290321
GTCATGA1288950.051.266843
ATGGGAC632500.051.2445079
TACATGA1231500.051.244093
ATGGGGG3583300.051.185169
Produced by FastQC (version 0.11.5)