##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528051_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14671238
Sequences flagged as poor quality	0
Sequence length	59
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.951905353863115	31.0	31.0	33.0	30.0	34.0
2	31.507087268300058	31.0	31.0	34.0	30.0	34.0
3	31.754681983892567	31.0	31.0	34.0	30.0	34.0
4	35.52077036716329	37.0	35.0	37.0	33.0	37.0
5	35.4202962967406	37.0	35.0	37.0	33.0	37.0
6	35.52688007651433	37.0	35.0	37.0	33.0	37.0
7	35.47804868273557	37.0	35.0	37.0	33.0	37.0
8	35.39039513911505	37.0	35.0	37.0	33.0	37.0
9	36.41731004568258	38.0	35.0	39.0	32.0	39.0
10	37.06046674452422	39.0	37.0	39.0	33.0	39.0
11	37.056197984110135	39.0	37.0	39.0	34.0	39.0
12	36.990126668247086	39.0	37.0	39.0	34.0	39.0
13	36.988376713676104	39.0	37.0	39.0	33.0	39.0
14	37.38247563020926	40.0	37.0	40.0	32.0	40.0
15	36.60639204407972	39.0	36.0	40.0	31.0	40.0
16	35.998764794082135	38.0	35.0	40.0	30.0	40.0
17	36.07000854324632	38.0	35.0	40.0	30.0	40.0
18	36.23620692405099	38.0	35.0	40.0	30.0	40.0
19	36.442793580200934	39.0	35.0	40.0	30.0	40.0
20	36.45918551658694	39.0	35.0	40.0	30.0	40.0
21	36.299302621905525	39.0	35.0	40.0	30.0	40.0
22	36.31646606782604	39.0	35.0	40.0	30.0	40.0
23	36.33676810368696	39.0	35.0	40.0	30.0	40.0
24	36.30921187428082	38.0	35.0	40.0	30.0	40.0
25	36.30962295070123	38.0	35.0	40.0	30.0	40.0
26	35.996822831174846	38.0	35.0	40.0	30.0	40.0
27	35.84651233931315	38.0	35.0	40.0	29.0	40.0
28	35.823562128840116	38.0	35.0	40.0	29.0	40.0
29	35.7951870183007	38.0	35.0	40.0	29.0	40.0
30	35.699148360894974	38.0	34.0	40.0	29.0	40.0
31	35.70269107487725	38.0	34.0	40.0	29.0	40.0
32	35.67496982872202	38.0	34.0	40.0	29.0	40.0
33	35.46118173531095	38.0	34.0	40.0	28.0	40.0
34	35.3469347303888	38.0	34.0	40.0	27.0	40.0
35	35.24813332044644	38.0	34.0	40.0	27.0	40.0
36	35.106897659215946	38.0	34.0	40.0	26.0	40.0
37	35.07298177563475	38.0	33.0	40.0	27.0	40.0
38	34.94611838482887	38.0	33.0	40.0	26.0	40.0
39	34.73997838491885	37.0	33.0	40.0	26.0	40.0
40	34.74105157315286	37.0	33.0	40.0	26.0	40.0
41	34.58500680038045	37.0	33.0	40.0	26.0	40.0
42	34.5923172945596	37.0	33.0	40.0	26.0	40.0
43	34.44170955443569	37.0	33.0	40.0	26.0	40.0
44	34.25185236583307	36.0	33.0	39.0	25.0	40.0
45	34.149966076482436	36.0	33.0	39.0	25.0	40.0
46	34.0388820629861	36.0	33.0	39.0	25.0	40.0
47	33.7670805967431	35.0	32.0	39.0	24.0	40.0
48	33.63930603538706	35.0	32.0	39.0	24.0	40.0
49	33.45375863986393	35.0	32.0	39.0	23.0	40.0
50	33.168628850544174	35.0	32.0	38.0	23.0	40.0
51	32.99795375141484	35.0	31.0	38.0	23.0	40.0
52	32.39975494910518	35.0	31.0	38.0	20.0	40.0
53	32.41459139303718	35.0	31.0	38.0	21.0	40.0
54	32.43592578894842	35.0	31.0	37.0	22.0	40.0
55	32.45005881575911	35.0	31.0	37.0	23.0	39.0
56	32.30470584690944	34.0	31.0	37.0	22.0	39.0
57	32.05763678566185	34.0	31.0	37.0	22.0	39.0
58	31.883736532663434	34.0	30.0	36.0	22.0	39.0
59	31.08346241809996	34.0	29.0	36.0	20.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	10.0
13	23.0
14	86.0
15	373.0
16	1448.0
17	3778.0
18	8510.0
19	16830.0
20	28273.0
21	44288.0
22	64482.0
23	91275.0
24	123314.0
25	163400.0
26	210015.0
27	267030.0
28	331756.0
29	402858.0
30	491010.0
31	606431.0
32	753109.0
33	954053.0
34	1300551.0
35	1602886.0
36	1874276.0
37	2475504.0
38	2603796.0
39	251872.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.348335430179787	20.609739955142164	33.992850501096086	19.049074113581963
2	22.083303399481352	23.14231423414984	35.857771511851965	18.916610854516843
3	24.007483213073087	21.598293204704333	36.226322550285126	18.167901031937454
4	26.208333611655675	22.0954836940141	37.866477252976196	13.82970544135403
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.54266981423108	0.3537533778676346	0.046526407655577535	0.057050400245705235
12	99.58801704396043	0.2852997136301654	0.08576644997511457	0.040916792434285365
13	98.74315309996334	0.6527942631698839	0.21527154013860317	0.38878109672816974
14	65.32427597452921	22.406445863668765	4.362856086173505	7.9064220756285195
15	35.74495213014743	26.27065282425382	16.253236434444045	21.731158611154697
16	30.893193880434627	17.36562381443202	25.886254452419077	25.854927852714273
17	24.995068582487722	17.258795747161894	29.098007952703103	28.648127717647277
18	25.117362283946314	17.130060871482012	31.29606376776111	26.456513076810563
19	26.325924233524123	17.61271952646396	28.064291506960764	27.997064733051158
20	29.726271225373075	17.27785344358806	24.529054739620474	28.46682059141839
21	31.108295019138808	18.5725908065836	21.42385666431149	28.8952575099661
22	29.94605499549527	20.66554983294525	21.48374254442604	27.90465262713344
23	29.48212686618539	21.046478831575087	20.3556509682414	29.11574333399813
24	29.535632916595038	23.10005467841228	20.80226631181363	26.56204609317905
25	32.038932229168395	20.13795972773395	22.28948913513638	25.533618907961277
26	30.878457564385503	18.921095820270928	22.799964120273966	27.400482495069607
27	31.799954441472494	20.16234758102895	21.322863142156102	26.714834835342455
28	30.800291018385767	19.60322639439153	21.189541059861476	28.406941527361223
29	29.286921798964748	19.678843734932254	21.5485496179668	29.485684848136195
30	28.748078383023984	20.469765400847564	21.77253889549062	29.009617320637837
31	28.731706213204365	19.72060571848129	21.256106676205512	30.291581392108828
32	29.479107352767368	21.79407763680202	20.187757842930502	28.539057167500108
33	30.046373727970334	22.275911548841346	20.213645228848446	27.464069494339878
34	29.014886132990274	22.08795876666986	21.968623234112894	26.92853186622697
35	31.75112420642348	22.449352944857143	19.035660112663976	26.763862736055405
36	34.66688359905278	21.5980955390404	19.69837855537481	24.036642306532006
37	33.652736054039885	21.387002242073912	20.49408509356879	24.46617661031741
38	30.46243268632136	23.15708463048585	23.782860042213205	22.59762264097958
39	29.814770914356377	25.191854975019833	23.224611310920046	21.768762799703747
40	30.83475300448401	25.472881020674603	19.300484389933555	24.391881584907832
41	30.34782068152667	25.813063628304576	18.94444081678724	24.894674873381508
42	32.472535719207876	24.243741393875553	17.894965646389213	25.388757240527347
43	32.36034341478204	22.627626925553248	18.516910433870677	26.495119225794035
44	29.97937188395417	22.604370537782835	19.52554378846557	27.890713789797427
45	27.376885304430342	23.01702146744535	20.83040981272337	28.775683415400938
46	27.323665528430524	20.051900187291626	22.6795312024793	29.94490308179855
47	26.710636143998208	18.601184167280223	24.526900865489335	30.16127882323223
48	27.37270706125822	18.94341159212331	23.287250878214913	30.39663046840355
49	28.47007185078723	19.712419633571482	22.969465835125842	28.84804268051544
50	26.782647790186488	22.51618438743888	22.47502221693902	28.226145605435615
51	27.62653703797866	21.923105602949118	22.556651320086278	27.89370603898594
52	28.60779029009004	21.116527453238778	23.871141617360443	26.404540639310735
53	28.21233627318976	22.08291488421086	22.41427069753759	27.290478145061787
54	26.204080391852415	22.475288043176725	23.15477398703504	28.16585757793582
55	26.99961652861197	21.598927098040395	22.790074021019905	28.61138235232773
56	27.387743283831945	21.163817259320584	22.928228687994835	28.520210768852632
57	26.642639155605004	21.49669305344239	24.578396178972763	27.282271611979848
58	27.014652751185686	19.764242117809008	24.544309076030256	28.676796054975046
59	25.979723047230234	20.03149291150481	23.86938307455717	30.119400966707786
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	2.0
5	3.0
6	7.0
7	11.0
8	420.0
9	1342.5
10	1856.0
11	3605.5
12	5355.0
13	6806.5
14	8268.0
15	8278.0
16	8049.0
17	7820.0
18	7956.0
19	8428.0
20	8764.0
21	11209.0
22	13654.0
23	19288.5
24	24923.0
25	28405.0
26	41082.5
27	50278.0
28	55699.0
29	61120.0
30	67357.5
31	80715.5
32	87836.0
33	102251.0
34	116666.0
35	129322.0
36	143724.5
37	145471.0
38	164239.5
39	183008.0
40	202551.5
41	222095.0
42	253130.0
43	297737.0
44	311309.0
45	344012.5
46	376716.0
47	395428.5
48	466892.5
49	519644.0
50	587971.5
51	656299.0
52	718305.5
53	876350.5
54	972389.0
55	1167919.5
56	1363450.0
57	1453617.0
58	1520378.0
59	1496972.0
60	1403152.0
61	1309332.0
62	1154535.5
63	999739.0
64	897244.5
65	668324.0
66	541898.0
67	465197.0
68	388496.0
69	336336.5
70	245342.5
71	206508.0
72	169006.5
73	131505.0
74	94874.5
75	38233.5
76	18223.0
77	14080.5
78	9938.0
79	7465.0
80	4992.0
81	3005.0
82	802.5
83	587.0
84	541.5
85	496.0
86	381.0
87	177.5
88	89.0
89	55.0
90	21.0
91	10.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1.4671238E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.487469862556502
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.83421607311936	7.574247189945438
2	8.077114072274393	1.5326275267426863
3	3.3392702664826834	0.9504367804855646
4	1.9646680020838654	0.7455891383879907
5	1.2486121916189241	0.5923085269002584
6	0.8540547150717422	0.48616910221304604
7	0.6382848262010841	0.423899563661693
8	0.4659366446215737	0.35364478989663034
9	0.3368752600447957	0.28764844894042946
>10	1.4248681256437328	2.399027724128324
>50	0.18241985469209565	1.2524340566168402
>100	0.923155323272901	26.335663736207643
>500	0.5480460274401691	35.97924831142913
>1k	0.1623238021710525	21.006410358662926
>5k	1.5481526196571533E-4	0.08064474578143772
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.006570679311452789	0.0	0.0	0.0	0.0
12	0.01158048148356669	0.0	0.0	0.0	0.0
13	0.01808981627862625	0.0	0.0	0.0	0.0
14	0.04460427947525628	0.0	0.0	0.0	0.0
15	0.06141949302437872	0.0	0.0	0.0	0.0
16	0.07319082411450213	0.0	0.0	0.0	0.0
17	0.08072938357349257	0.0	0.0	0.0	0.0
18	0.09068082734395011	0.0	0.0	0.0	0.0
19	0.09958941433572273	0.0	0.0	0.0	0.0
20	0.10986802886027751	0.0	0.0	0.0	0.0
21	0.12011256309794716	0.0	0.0	0.0	0.0
22	0.1307524286634843	0.0	0.0	0.0	0.0
23	0.14178762555688892	0.0	0.0	0.0	0.0
24	0.15294551148308003	0.0	0.0	0.0	0.0
25	0.16509172572893985	0.0	0.0	0.0	0.0
26	0.17771506399119147	0.0	0.0	0.0	0.0
27	0.19001804755672289	0.0	0.0	0.0	0.0
28	0.20270273033536773	0.0	0.0	0.0	0.0
29	0.21842737470416607	0.0	0.0	0.0	0.0
30	0.23672848876148012	0.0	0.0	0.0	0.0
31	0.25239860467126224	0.0	0.0	0.0	0.0
32	0.2660375354826907	0.0	0.0	0.0	0.0
33	0.27726358198265205	0.0	0.0	0.0	0.0
34	0.2887281904908093	0.0	0.0	0.0	0.0
35	0.30013827053995035	0.0	0.0	0.0	0.0
36	0.31186188922843455	0.0	0.0	0.0	0.0
37	0.3218542293431543	0.0	0.0	0.0	0.0
38	0.33355739985950744	0.0	0.0	0.0	0.0
39	0.3458740155397929	0.0	0.0	0.0	0.0
40	0.36097158262990486	0.0	0.0	0.0	0.0
41	0.37480817910526704	0.0	0.0	0.0	0.0
42	0.38695439335112686	0.0	0.0	0.0	0.0
43	0.39845308214616926	0.0	0.0	0.0	0.0
44	0.4097677373920319	0.0	0.0	0.0	0.0
45	0.42162086117067965	0.0	0.0	0.0	0.0
46	0.4330036769903126	0.0	0.0	0.0	0.0
47	0.44366399072798085	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATGA	75070	0.0	51.99041	3
TTCATGA	216700	0.0	51.780777	3
ATGGGGC	221160	0.0	51.77781	9
TGATGGG	1475620	0.0	51.668015	7
TCATGAT	572730	0.0	51.662342	4
CATGATG	1498325	0.0	51.65318	5
GATGGGG	970740	0.0	51.63725	8
TTCGCAT	8870	0.0	51.6257	1
ATGATGG	1494745	0.0	51.599957	6
CACATGA	72950	0.0	51.492462	3
TTTCATG	81740	0.0	51.37901	2
GCATGAT	395435	0.0	51.327976	4
ATGGGAG	141135	0.0	51.32515	9
ATGGGGA	281960	0.0	51.306393	9
ACATGAT	333770	0.0	51.297745	4
TACGCAT	6200	0.0	51.29032	1
GTCATGA	128895	0.0	51.26684	3
ATGGGAC	63250	0.0	51.244507	9
TACATGA	123150	0.0	51.24409	3
ATGGGGG	358330	0.0	51.18516	9
>>END_MODULE