FastQCFastQC Report
Fri 17 Jun 2016
SRR1528050_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528050_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274361
Sequences flagged as poor quality0
Sequence length52
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAATTAGGGCAATTGCAGGGAGAGGGGGCACCCCCAGACTACCAGGAA13510.49241692514606666No Hit
TATGGAATTAGGGCTTAGATACCATAGAGAATTGCACTGGAAGTAAAATATA10890.3969223030970145No Hit
TGATGAATTAGGGACGCAGAGTGCTTGAATTAGGGCTTTTGTTGCAGACGAC9720.35427775813617823No Hit
GTTGGAATTAGGGGGGCAGACTGTCTGGACAAAGTCTGTGGTTTAGCCAAGG5480.19973684306442968No Hit
CTACGAATTAGGGATCCACATGAGTCAGCAATGCTTTGTTATCCATCTCGAC4910.17896129551940693No Hit
ATAAGAATTAGGGCCGTGTCTACTAAAAATACAAAAAAAAATTTAGCCGGGT4710.17167162971413577No Hit
TGGCGAATTAGGGACAATCTGGTATTCTTCATGCAGCCGATGAAAGAGATTT4550.16583989706991883No Hit
TGTAGAATTAGGGGGCACTCAAAGTATGCCTATTCCAGAGACACCAGACATC4500.16401748061860105No Hit
GTTCGAATTAGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG4070.14834469913726805No Hit
GGTTGAATTAGGGAGAATCACCTCGCCAACCACATCTGCTTTATGGGGGAAA4070.14834469913726805No Hit
TTTAGAATTAGGGTGGATCACCTGAGGTTGGAAGTTGAAGACCAGCCTGGCC4050.14761573255674093No Hit
ACCTGAATTAGGGTGTTTTTACCCACCAACATTCTTCTCTGTTCCTTGAGGT4010.1461577993956867No Hit
TGGGGAATTAGGGACCCAGCCACACCAGCTTGACTAAGGTGTTTGAGCCATT3900.14214848320278756No Hit
TTAGGAATTAGGGGGCACATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGG3860.14069055004173334No Hit
TTTGGAATTAGGGGAACCTGCCCGGGACCGCTGGCGGCCCCAACTGGATTTG3840.13996158346120624No Hit
CGTTGAATTAGGGGTGTCCGTTGTCGTCTGGGCTCGGGTAGATCGATGCACA3560.12975605133382662No Hit
GTTTGAATTAGGGTGGTGGCGGGCACCTGTAATCCCAGCTACTTGGGAGGCT3460.12611121843119102No Hit
GGTCGAATTAGGGGAAGTCCAAGTTTATCCATCGTACCTGGTCATTCTCATT3420.12465328527013679No Hit
TATTGAATTAGGGCACCATCTTCACAAAGCCCTAAAAATGAAGAATAACAAC3170.11554120301354785No Hit
GAGGGAATTAGGGAACTGTGGATCGATATCCTGGCTCAGACGACGGGTCATA3140.11444775314275718No Hit
AGCAGAATTAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC3020.11007395365959449No Hit
GTTCGAATTAGGGAGCCGTTCGGCCGAATGACTCGGTGTCGCATCGCGGCTT2980.10861602049854024No Hit
GGGTGAATTAGGGGGAGTAGTCGAATTAGGGGTTTTGAATCGTCGGGCCATC2950.10752257062774956No Hit
GAGTGAATTAGGGCACTTTCACTGAATAACATAACTCAAATTAGTTCTGTGA2940.10715808733748602No Hit
TTCAGAATTAGGGAATCCTTTGCTTTGGATAGGAATTATTGCTTCAATTTAC2940.10715808733748602No Hit
CATGGAATTAGGGTGCCAGGGATGTGATAGACACTAAAGATGCATTCGTTAA2860.10424222101537756No Hit
GTGTGAATTAGGGATTGAGTTTCGAGCAAACGAAGCAAACTTGTGAAACAGC2790.10169083798353264No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGT850.046.00000436
TAGAAGC405.5842975E-946.00000436
CTTTCAC206.303013E-446.00000416
TGTGAGT206.303013E-446.00000424
GATGGCA206.303013E-446.00000441
AACGTTA206.303013E-446.00000442
TTCACTG206.303013E-446.00000418
TGTGAAC206.303013E-446.00000423
ACTGTGG453.074092E-1046.00000415
ACAAAGC405.5842975E-946.00000424
TGTTGCA850.046.00000440
GTGTCTC206.303013E-446.00000429
TGCATGC405.5842975E-946.00000431
CCTACTC206.303013E-446.00000429
TTCACAA405.5842975E-946.00000421
ACTCGGT405.5842975E-946.00000431
GTGTCGC405.5842975E-946.00000436
TGGCAAT206.303013E-446.00000443
CCGTGCC405.5842975E-946.00000435
CTTTATG453.074092E-1046.00000439
Produced by FastQC (version 0.11.5)