##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528050_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	274361
Sequences flagged as poor quality	0
Sequence length	52
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.77496801659128	31.0	31.0	34.0	27.0	34.0
2	31.685691479474123	31.0	31.0	34.0	28.0	34.0
3	32.07925689146781	33.0	31.0	34.0	30.0	34.0
4	35.70395209231633	37.0	35.0	37.0	33.0	37.0
5	35.78967856218632	37.0	35.0	37.0	35.0	37.0
6	35.35133637798375	37.0	35.0	37.0	33.0	37.0
7	35.57621892324347	37.0	35.0	37.0	33.0	37.0
8	35.774217181013334	37.0	35.0	37.0	33.0	37.0
9	37.97521149142918	39.0	38.0	39.0	35.0	39.0
10	37.34848611865389	39.0	37.0	39.0	33.0	39.0
11	38.160970400311996	39.0	39.0	39.0	37.0	39.0
12	38.20264177488783	39.0	39.0	39.0	37.0	39.0
13	38.12922390573004	39.0	39.0	39.0	37.0	39.0
14	38.35300935628606	40.0	38.0	40.0	34.0	40.0
15	37.5336764336039	40.0	37.0	40.0	32.0	40.0
16	36.889302779914054	39.0	35.0	40.0	32.0	40.0
17	36.70076650835942	39.0	35.0	40.0	31.0	40.0
18	36.66372042673704	39.0	35.0	40.0	31.0	40.0
19	37.034348905274435	39.0	35.0	40.0	32.0	40.0
20	37.089611861744196	39.0	35.0	40.0	32.0	40.0
21	37.073454317486814	39.0	35.0	40.0	32.0	40.0
22	37.16102871763844	39.0	35.0	40.0	32.0	40.0
23	37.029238849544946	39.0	35.0	40.0	32.0	40.0
24	37.21585429415988	39.0	35.0	40.0	32.0	40.0
25	37.24831882082366	40.0	36.0	40.0	32.0	40.0
26	37.14185689657058	40.0	36.0	40.0	32.0	40.0
27	37.028619227951495	39.0	35.0	40.0	32.0	40.0
28	36.72883172170972	39.0	35.0	40.0	31.0	40.0
29	36.50590645171872	39.0	35.0	40.0	31.0	40.0
30	36.25582353177018	38.0	35.0	40.0	31.0	40.0
31	36.089302050947474	38.0	35.0	40.0	30.0	40.0
32	35.97379729626295	38.0	35.0	40.0	30.0	40.0
33	35.77629838060074	38.0	35.0	40.0	30.0	40.0
34	35.46528843385175	38.0	35.0	40.0	27.0	40.0
35	35.264684120556495	38.0	35.0	40.0	26.0	40.0
36	34.913129052598585	38.0	35.0	40.0	25.0	40.0
37	34.66029428380856	37.0	34.0	40.0	23.0	40.0
38	34.30879388834419	37.0	34.0	40.0	21.0	40.0
39	33.85176464584981	37.0	33.0	40.0	20.0	40.0
40	33.23554732633283	36.0	33.0	40.0	16.0	40.0
41	32.57187792725642	35.0	33.0	40.0	12.0	40.0
42	31.83677344812127	35.0	31.0	40.0	10.0	40.0
43	31.206450625271085	35.0	29.0	40.0	9.0	40.0
44	30.607655607028697	35.0	25.0	40.0	8.0	40.0
45	30.07886689434723	35.0	23.0	40.0	8.0	40.0
46	29.562652855179856	35.0	22.0	40.0	8.0	40.0
47	29.081068373420422	35.0	20.0	39.0	8.0	40.0
48	28.632188977296337	35.0	18.0	39.0	8.0	40.0
49	28.196835556073932	35.0	15.0	39.0	8.0	40.0
50	27.73982818257697	35.0	13.0	38.0	8.0	40.0
51	27.280276715713967	34.0	10.0	38.0	8.0	40.0
52	26.44937144856594	33.0	10.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	7.0
16	22.0
17	86.0
18	237.0
19	465.0
20	832.0
21	1232.0
22	1702.0
23	2336.0
24	3195.0
25	4208.0
26	5731.0
27	7381.0
28	9280.0
29	11625.0
30	13739.0
31	14273.0
32	12545.0
33	12289.0
34	14428.0
35	20679.0
36	22112.0
37	39508.0
38	54489.0
39	21959.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.215726725008295	19.8803765841355	41.5700482211393	5.333848469716906
2	20.698277087486925	21.77678314337679	37.925215318503724	19.59972445063256
3	21.54387832089838	22.776196325279468	35.74779214246922	19.932133211352927
4	24.0070563964995	21.172834331410076	33.665134621903256	21.15497465018716
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.14783806736381	0.5529211513298173	0.12684018501171815	0.17240059629466287
12	98.6867667051804	0.805508071482463	0.3484460254919613	0.15927919784517477
13	96.49367074766457	2.214964954931641	0.7737980252295333	0.5175662721742522
14	48.97197487981163	32.41386348642846	10.183298646673542	8.430862987086357
15	35.39242093446226	38.24413819748433	18.79640327889168	7.567037589161725
16	41.98628813862029	29.46373573503523	17.91945648251756	10.63051964382693
17	21.65249434139692	34.32849421018294	19.406548306792875	24.612463141627273
18	16.4688129872686	47.5501255644935	20.852818002558674	15.128243445679232
19	22.77036459263525	44.37912093920055	23.06924089065137	9.781273577512838
20	15.115486530520009	43.00100961871403	27.250593196554906	14.632910654211056
21	23.050287759557662	43.10306493998783	15.057169204077839	18.789478096376673
22	22.946774505122814	46.11442588414534	16.122918344808483	14.815881265923364
23	15.499287435167536	45.93036182256224	22.945316571961758	15.625034170308464
24	15.927919784517478	51.23906094525097	16.0037323088923	16.829286961339257
25	17.936222713869682	47.00084924606631	20.081935843651248	14.980992196412757
26	15.197130787539045	48.10669154872595	16.29458997452262	20.401587689212388
27	15.598426890119221	54.88717419749891	14.124820947583657	15.389577964798203
28	15.67278148133299	54.216524943413965	13.446517544403177	16.664176030849866
29	18.171678919379943	51.73330028684835	12.74634514380688	17.34867564996483
30	23.012381497370253	50.953670528974605	13.892280608395508	12.14166736525964
31	16.139320092870342	54.870043482856524	13.558778397804353	15.431858026468776
32	18.159650970801245	58.9059669559449	13.549301832257502	9.385080240996352
33	20.110365540291806	55.07779895830676	12.747803076967937	12.064032424433503
34	17.177004020250692	60.25054581372717	11.574166882319279	10.99828328370286
35	18.238743844788434	55.460506413083486	15.264924679528066	11.035825062600004
36	16.109432463068732	53.78862156064456	18.944383494738684	11.157562481548034
37	15.442063558596155	56.918075090847466	16.453869172367792	11.18599217818859
38	15.669501131720617	57.44183757895619	16.15134804144904	10.737313247874152
39	17.209078549793887	53.35197057890881	17.875354004395668	11.563596866901637
40	19.526827792579848	53.10375745823933	15.837527928532117	11.531886820648706
41	22.568076366538975	53.45767073308524	13.39986368324944	10.574389217126342
42	19.517715710323262	53.33228848123458	14.595733358604173	12.554262449837989
43	19.778321262861702	50.95184811252328	16.394822879345096	12.875007745269917
44	21.22240405888592	46.13265004865852	20.78575307715018	11.859192815305382
45	22.757243194185765	46.09365033660032	17.911802333422024	13.237304135791895
46	24.859582812425966	47.25161374976764	14.730227692711429	13.158575745094966
47	27.045389104136518	42.93066434369317	15.071019569107854	14.952926983062461
48	26.937137566928243	44.16261786478399	15.625398653598726	13.27484591468904
49	26.012078976239334	43.592565998811786	14.416772063084768	15.978582961864113
50	24.444800828106036	46.29411614624527	15.13917794438714	14.121905081261549
51	23.539424335091358	45.325319560724736	16.91894985074409	14.21630625343981
52	23.15124963096067	45.61945757596743	15.48434362026673	15.744949172805173
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	5.0
6	8.0
7	69.5
8	131.0
9	240.5
10	350.0
11	488.5
12	627.0
13	802.5
14	1206.0
15	1434.0
16	1931.5
17	2429.0
18	3306.0
19	4183.0
20	5896.0
21	7609.0
22	10253.5
23	12898.0
24	15961.5
25	19025.0
26	18982.5
27	18940.0
28	17788.0
29	16636.0
30	15423.0
31	14210.0
32	14548.5
33	14887.0
34	13447.5
35	12008.0
36	11473.5
37	10939.0
38	11558.0
39	12566.0
40	12955.0
41	14936.0
42	16917.0
43	18258.0
44	19599.0
45	19884.5
46	20170.0
47	18364.0
48	16558.0
49	14862.5
50	13167.0
51	11414.5
52	9662.0
53	7475.5
54	5289.0
55	4366.5
56	3444.0
57	3359.5
58	3275.0
59	2323.5
60	1372.0
61	1297.0
62	1222.0
63	944.0
64	481.0
65	296.0
66	213.5
67	131.0
68	104.5
69	78.0
70	58.0
71	38.0
72	24.0
73	10.0
74	9.0
75	8.0
76	8.5
77	9.0
78	7.0
79	5.0
80	4.0
81	3.0
82	7.5
83	12.0
84	8.0
85	4.0
86	2.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	274361.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.54558241014099
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.32947968214357	45.690166863346704
2	5.914646380050822	6.334063703397068
3	2.4186121230288733	3.885179842554258
4	1.3827148772913032	2.961530936469179
5	0.96370512359762	2.5801076057335735
6	0.6561355099790717	2.1079894813082563
7	0.49191603791432825	1.8437951522908194
8	0.37421107741750653	1.6029880067717397
9	0.3065505734763323	1.477298609545764
>10	1.996822628526809	18.667808287350397
>50	0.08192032392088702	3.0606604581248753
>100	0.08055498518887225	8.34087547276883
>500	0.0013653387320147839	0.5556197177056982
>1k	0.0013653387320147839	0.8919158626328312
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACGGAATTAGGGCAATTGCAGGGAGAGGGGGCACCCCCAGACTACCAGGAA	1351	0.49241692514606666	No Hit
TATGGAATTAGGGCTTAGATACCATAGAGAATTGCACTGGAAGTAAAATATA	1089	0.3969223030970145	No Hit
TGATGAATTAGGGACGCAGAGTGCTTGAATTAGGGCTTTTGTTGCAGACGAC	972	0.35427775813617823	No Hit
GTTGGAATTAGGGGGGCAGACTGTCTGGACAAAGTCTGTGGTTTAGCCAAGG	548	0.19973684306442968	No Hit
CTACGAATTAGGGATCCACATGAGTCAGCAATGCTTTGTTATCCATCTCGAC	491	0.17896129551940693	No Hit
ATAAGAATTAGGGCCGTGTCTACTAAAAATACAAAAAAAAATTTAGCCGGGT	471	0.17167162971413577	No Hit
TGGCGAATTAGGGACAATCTGGTATTCTTCATGCAGCCGATGAAAGAGATTT	455	0.16583989706991883	No Hit
TGTAGAATTAGGGGGCACTCAAAGTATGCCTATTCCAGAGACACCAGACATC	450	0.16401748061860105	No Hit
GTTCGAATTAGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG	407	0.14834469913726805	No Hit
GGTTGAATTAGGGAGAATCACCTCGCCAACCACATCTGCTTTATGGGGGAAA	407	0.14834469913726805	No Hit
TTTAGAATTAGGGTGGATCACCTGAGGTTGGAAGTTGAAGACCAGCCTGGCC	405	0.14761573255674093	No Hit
ACCTGAATTAGGGTGTTTTTACCCACCAACATTCTTCTCTGTTCCTTGAGGT	401	0.1461577993956867	No Hit
TGGGGAATTAGGGACCCAGCCACACCAGCTTGACTAAGGTGTTTGAGCCATT	390	0.14214848320278756	No Hit
TTAGGAATTAGGGGGCACATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGG	386	0.14069055004173334	No Hit
TTTGGAATTAGGGGAACCTGCCCGGGACCGCTGGCGGCCCCAACTGGATTTG	384	0.13996158346120624	No Hit
CGTTGAATTAGGGGTGTCCGTTGTCGTCTGGGCTCGGGTAGATCGATGCACA	356	0.12975605133382662	No Hit
GTTTGAATTAGGGTGGTGGCGGGCACCTGTAATCCCAGCTACTTGGGAGGCT	346	0.12611121843119102	No Hit
GGTCGAATTAGGGGAAGTCCAAGTTTATCCATCGTACCTGGTCATTCTCATT	342	0.12465328527013679	No Hit
TATTGAATTAGGGCACCATCTTCACAAAGCCCTAAAAATGAAGAATAACAAC	317	0.11554120301354785	No Hit
GAGGGAATTAGGGAACTGTGGATCGATATCCTGGCTCAGACGACGGGTCATA	314	0.11444775314275718	No Hit
AGCAGAATTAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC	302	0.11007395365959449	No Hit
GTTCGAATTAGGGAGCCGTTCGGCCGAATGACTCGGTGTCGCATCGCGGCTT	298	0.10861602049854024	No Hit
GGGTGAATTAGGGGGAGTAGTCGAATTAGGGGTTTTGAATCGTCGGGCCATC	295	0.10752257062774956	No Hit
GAGTGAATTAGGGCACTTTCACTGAATAACATAACTCAAATTAGTTCTGTGA	294	0.10715808733748602	No Hit
TTCAGAATTAGGGAATCCTTTGCTTTGGATAGGAATTATTGCTTCAATTTAC	294	0.10715808733748602	No Hit
CATGGAATTAGGGTGCCAGGGATGTGATAGACACTAAAGATGCATTCGTTAA	286	0.10424222101537756	No Hit
GTGTGAATTAGGGATTGAGTTTCGAGCAAACGAAGCAAACTTGTGAAACAGC	279	0.10169083798353264	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.1713071464238722	0.0	0.0	0.0	0.0
11	0.18661544461494162	0.0	0.0	0.0	0.0
12	0.2103068584820729	0.0	0.0	0.0	0.0
13	0.31090424659481486	0.0	0.0	0.0	0.0
14	0.6830416859539075	0.0	0.0	0.0	0.0
15	1.1860286265176172	0.0	0.0	0.0	0.0
16	1.394877551838636	0.0	0.0	0.0	0.0
17	1.5516053666519658	0.0	0.0	0.0	0.0
18	1.788155022033015	0.0	0.0	0.0	0.0
19	2.005751546320359	0.0	0.0	0.0	0.0
20	2.2306377364129744	0.0	0.0	0.0	0.0
21	2.4329259625092488	0.0	0.0	0.0	0.0
22	2.6844194327911035	0.0	0.0	0.0	0.0
23	2.885978692306851	0.0	0.0	0.0	0.0
24	3.1239862808489547	0.0	0.0	0.0	0.0
25	3.3966197819660957	0.0	0.0	0.0	0.0
26	3.7425144244262123	0.0	0.0	0.0	0.0
27	4.111735997463196	0.0	0.0	0.0	0.0
28	4.470023071792274	0.0	0.0	0.0	0.0
29	4.878973323467986	0.0	0.0	0.0	0.0
30	5.3498857344885025	0.0	0.0	0.0	0.0
31	5.76867703500133	0.0	0.0	0.0	0.0
32	6.148833106746221	0.0	0.0	0.0	0.0
33	6.445886988311021	0.0	0.0	0.0	0.0
34	6.707950474010519	0.0	0.0	0.0	0.0
35	6.943406679520777	0.0	0.0	0.0	0.0
36	7.155900437744432	0.0	0.0	0.0	0.0
37	7.365842812936241	0.0	0.0	0.0	0.0
38	7.58599072025543	0.0	0.0	0.0	0.0
39	7.81853105944358	0.0	0.0	0.0	0.0
40	8.065286246952008	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTTGT	85	0.0	46.000004	36
TAGAAGC	40	5.5842975E-9	46.000004	36
CTTTCAC	20	6.303013E-4	46.000004	16
TGTGAGT	20	6.303013E-4	46.000004	24
GATGGCA	20	6.303013E-4	46.000004	41
AACGTTA	20	6.303013E-4	46.000004	42
TTCACTG	20	6.303013E-4	46.000004	18
TGTGAAC	20	6.303013E-4	46.000004	23
ACTGTGG	45	3.074092E-10	46.000004	15
ACAAAGC	40	5.5842975E-9	46.000004	24
TGTTGCA	85	0.0	46.000004	40
GTGTCTC	20	6.303013E-4	46.000004	29
TGCATGC	40	5.5842975E-9	46.000004	31
CCTACTC	20	6.303013E-4	46.000004	29
TTCACAA	40	5.5842975E-9	46.000004	21
ACTCGGT	40	5.5842975E-9	46.000004	31
GTGTCGC	40	5.5842975E-9	46.000004	36
TGGCAAT	20	6.303013E-4	46.000004	43
CCGTGCC	40	5.5842975E-9	46.000004	35
CTTTATG	45	3.074092E-10	46.000004	39
>>END_MODULE