SRR1528049_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1528049_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	272916
Sequences flagged as poor quality	0
Sequence length	52
%GC	37

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TACGGAATTAGGGCAATTGCAGGGAGAGGGGGCACCCCCAGACTACCAGGAA	1265	0.463512582626156	No Hit
TGATGAATTAGGGACGCAGAGTGCTTGAATTAGGGCTTTTGTTGCAGACGAC	951	0.3484588664644066	No Hit
TATGGAATTAGGGCTTAGATACCATAGAGAATTGCACTGGAAGTAAAATATA	936	0.3429626698324759	No Hit
GTTGGAATTAGGGGGGCAGACTGTCTGGACAAAGTCTGTGGTTTAGCCAAGG	535	0.19603101320552846	No Hit
CTACGAATTAGGGATCCACATGAGTCAGCAATGCTTTGTTATCCATCTCGAC	470	0.17221416113382873	No Hit
ATAAGAATTAGGGCCGTGTCTACTAAAAATACAAAAAAAAATTTAGCCGGGT	440	0.1612217678699673	No Hit
GTTCGAATTAGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG	425	0.1557255712380366	No Hit
TGGCGAATTAGGGACAATCTGGTATTCTTCATGCAGCCGATGAAAGAGATTT	413	0.15132861393249206	No Hit
TGTAGAATTAGGGGGCACTCAAAGTATGCCTATTCCAGAGACACCAGACATC	412	0.15096220082369666	No Hit
GGTTGAATTAGGGAGAATCACCTCGCCAACCACATCTGCTTTATGGGGGAAA	403	0.14766448284453823	No Hit
ACCTGAATTAGGGTGTTTTTACCCACCAACATTCTTCTCTGTTCCTTGAGGT	379	0.13887056823344912	No Hit
TTTAGAATTAGGGTGGATCACCTGAGGTTGGAAGTTGAAGACCAGCCTGGCC	377	0.13813774201585835	No Hit
TTTGGAATTAGGGGAACCTGCCCGGGACCGCTGGCGGCCCCAACTGGATTTG	364	0.1333743716015184	No Hit
TGGGGAATTAGGGACCCAGCCACACCAGCTTGACTAAGGTGTTTGAGCCATT	349	0.1278781749695877	No Hit
TTAGGAATTAGGGGGCACATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGG	331	0.12128273901127087	No Hit
GGTCGAATTAGGGGAAGTCCAAGTTTATCCATCGTACCTGGTCATTCTCATT	311	0.11395447683536326	No Hit
CGTTGAATTAGGGGTGTCCGTTGTCGTCTGGGCTCGGGTAGATCGATGCACA	303	0.11102317196500022	No Hit
TATTGAATTAGGGCACCATCTTCACAAAGCCCTAAAAATGAAGAATAACAAC	296	0.10845828020343257	No Hit
GGGTGAATTAGGGGGAGTAGTCGAATTAGGGGTTTTGAATCGTCGGGCCATC	291	0.10662621465945565	No Hit
AGCAGAATTAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC	290	0.10625980155066028	No Hit
GAGTGAATTAGGGCACTTTCACTGAATAACATAACTCAAATTAGTTCTGTGA	290	0.10625980155066028	No Hit
GTTTGAATTAGGGTGGTGGCGGGCACCTGTAATCCCAGCTACTTGGGAGGCT	286	0.10479414911547875	No Hit
GTTCGAATTAGGGAGCCGTTCGGCCGAATGACTCGGTGTCGCATCGCGGCTT	280	0.10259567046270648	No Hit
CATGGAATTAGGGTGCCAGGGATGTGATAGACACTAAAGATGCATTCGTTAA	273	0.10003077870113881	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTTTA	55	1.8189894E-12	46.000004	39
TTCACTG	55	1.8189894E-12	46.000004	18
TGTGAAC	35	1.0157419E-7	46.000004	23
ACTGTCA	35	1.0157419E-7	46.000004	22
TTGTTAT	75	0.0	46.000004	36
CCTACTC	35	1.0157419E-7	46.000004	29
CCGTGCC	55	1.8189894E-12	46.000004	35
CGATACC	35	1.0157419E-7	46.000004	43
ACTTGTG	35	1.0157419E-7	46.000004	39
GAAATGA	35	1.0157419E-7	46.000004	27
CGCCTGA	35	1.0157419E-7	46.000004	15
GATACCG	35	1.0157419E-7	46.000004	44
AACGAAG	35	1.0157419E-7	46.000004	29
CAACTTT	55	1.8189894E-12	46.000004	16
GCCTACC	55	1.8189894E-12	46.000004	39
TTCGATG	55	1.8189894E-12	46.000004	21
GACGGGT	35	1.0157419E-7	46.000004	42
GGATTTT	35	1.0157419E-7	46.000004	45
CATTCTC	35	1.0157419E-7	46.000004	43
CACTGTC	35	1.0157419E-7	46.000004	21

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content