##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528049_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	272916
Sequences flagged as poor quality	0
Sequence length	52
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.685060604728196	31.0	31.0	34.0	27.0	34.0
2	31.48049583021882	31.0	31.0	34.0	28.0	34.0
3	31.988868369754798	33.0	31.0	34.0	30.0	34.0
4	35.67121385334682	37.0	35.0	37.0	33.0	37.0
5	35.77126661683448	37.0	35.0	37.0	35.0	37.0
6	35.333472570314676	37.0	35.0	37.0	33.0	37.0
7	35.572263993316625	37.0	35.0	37.0	33.0	37.0
8	35.72614650661742	37.0	35.0	37.0	33.0	37.0
9	37.968111067141535	39.0	38.0	39.0	35.0	39.0
10	37.33878556039221	39.0	37.0	39.0	33.0	39.0
11	38.15107212475633	39.0	39.0	39.0	37.0	39.0
12	38.18481144381421	39.0	39.0	39.0	37.0	39.0
13	38.13094505269021	39.0	39.0	39.0	37.0	39.0
14	38.38854812469771	40.0	38.0	40.0	34.0	40.0
15	37.5584612115083	40.0	37.0	40.0	32.0	40.0
16	36.9166556742734	39.0	35.0	40.0	32.0	40.0
17	36.761545677058145	39.0	35.0	40.0	31.0	40.0
18	36.747358161485586	39.0	35.0	40.0	31.0	40.0
19	37.11991601811546	39.0	35.0	40.0	32.0	40.0
20	37.13686262439725	39.0	35.0	40.0	32.0	40.0
21	37.3036868487007	39.0	36.0	40.0	33.0	40.0
22	37.29873660760087	40.0	36.0	40.0	32.0	40.0
23	37.127207638980494	39.0	35.0	40.0	32.0	40.0
24	37.314371454953175	39.0	36.0	40.0	33.0	40.0
25	37.35373521523106	40.0	36.0	40.0	33.0	40.0
26	37.1934551290507	40.0	36.0	40.0	32.0	40.0
27	37.04213017924929	39.0	35.0	40.0	32.0	40.0
28	36.52080860044849	39.0	35.0	40.0	31.0	40.0
29	36.40153380527342	39.0	35.0	40.0	31.0	40.0
30	36.234269885239414	38.0	35.0	40.0	31.0	40.0
31	36.11762960031658	38.0	35.0	40.0	30.0	40.0
32	35.99451113163025	38.0	35.0	40.0	30.0	40.0
33	35.796728663764675	38.0	35.0	40.0	30.0	40.0
34	35.53018511190256	38.0	35.0	40.0	28.0	40.0
35	35.26069559864574	38.0	35.0	40.0	26.0	40.0
36	34.52013073619722	37.0	34.0	40.0	23.0	40.0
37	34.50539360096147	37.0	34.0	40.0	23.0	40.0
38	34.246167318882	37.0	33.0	40.0	21.0	40.0
39	33.841581292412314	37.0	33.0	40.0	20.0	40.0
40	33.25023450438963	36.0	33.0	40.0	16.0	40.0
41	32.59489366691583	35.0	33.0	40.0	12.0	40.0
42	31.697558955869205	35.0	31.0	40.0	10.0	40.0
43	31.075939116797844	35.0	28.0	40.0	9.0	40.0
44	30.492484867138607	35.0	25.0	40.0	8.0	40.0
45	29.75590657931378	35.0	23.0	40.0	8.0	40.0
46	29.383070248721218	35.0	20.0	40.0	8.0	40.0
47	28.98613859209427	35.0	20.0	39.0	8.0	40.0
48	28.537524366471736	35.0	17.0	39.0	8.0	40.0
49	28.134960940362603	35.0	15.0	39.0	8.0	40.0
50	27.626793592167555	35.0	12.0	38.0	8.0	40.0
51	27.231745298919815	34.0	10.0	38.0	8.0	40.0
52	26.493507159712145	33.0	10.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	2.0
15	9.0
16	28.0
17	95.0
18	186.0
19	389.0
20	705.0
21	1124.0
22	1622.0
23	2285.0
24	3191.0
25	4388.0
26	5628.0
27	7467.0
28	9330.0
29	11864.0
30	13978.0
31	14583.0
32	12738.0
33	12230.0
34	14697.0
35	20385.0
36	22124.0
37	39477.0
38	52779.0
39	21611.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.33003561535418	19.941667033079778	41.319307039528645	5.408990312037403
2	20.84524175936918	21.73159506954521	37.817496958771194	19.605666212314414
3	21.610312330533937	22.758284600389864	35.7234460420056	19.9079570270706
4	23.982837209984023	20.968722977033227	33.73785340544344	21.31058640753932
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.09752451303699	0.575268580808747	0.12751176186079233	0.19969514429348223
12	98.66552345776722	0.7855897052572953	0.37264213164490173	0.17624470533057793
13	96.42527371059228	2.2351199636518193	0.823696668572015	0.5159096571838954
14	49.10228788345132	32.371132509636666	10.242345630157265	8.284233976754752
15	35.478682085330284	38.156795497515716	18.787099327265533	7.577423089888464
16	41.72492635096513	29.8066071611778	17.872898620821058	10.595567867036012
17	21.682129299857834	34.38054199827053	19.479620102888802	24.457708598982837
18	16.406146946313154	47.57104750179542	20.865760893461722	15.1570446584297
19	22.534406190915888	44.63204795614768	23.003048557065178	9.830497295871258
20	15.088891820193759	43.233082706766915	27.102112005159096	14.575913467880227
21	22.55236043324686	43.602427120432665	14.94049451113163	18.90471793518885
22	22.815445045361944	46.33000630230547	16.1562532061147	14.698295446217886
23	15.409869703498513	46.11565463366018	22.861613097070162	15.612862565771152
24	15.873382286124668	51.39969807559835	15.896832725087574	16.830086913189408
25	18.02092951677439	47.094710460361426	20.11241554177842	14.771944481085756
26	15.119304108223774	48.125430535402835	16.276070292690793	20.4791950636826
27	15.55680136012546	55.08141699277434	13.953744009145671	15.408037637954536
28	15.927611426226385	54.19396444327192	13.398994562429465	16.479429568072227
29	18.23674683785487	51.74742411584516	12.646015623854959	17.369813422445002
30	22.84732298582714	51.251667179645025	13.829529965264037	12.071479869263802
31	16.140497442436498	54.76117193568717	13.580735464391974	15.517595157484354
32	18.059769306306702	58.82652537777191	13.621773732577056	9.491931583344327
33	20.086766624162745	55.251066262146594	12.656641604010025	12.005525509680634
34	17.242668073693004	60.27092585264331	11.545310645033636	10.941095428630055
35	18.358395989974937	55.455158363745625	15.106479649415938	11.079965996863503
36	16.59594893666916	52.90565595274737	18.924870656172597	11.573524454410881
37	15.375426871271747	56.87317709478374	16.650910902988468	11.100485130956045
38	15.69713758079409	57.505972533673365	16.136100485130957	10.660789400401589
39	17.40315701534538	53.216374269005854	17.88059329610576	11.49987541954301
40	19.37482595377332	53.14272523413798	15.871183807471898	11.611265004616806
41	22.75608612173709	53.328130267188435	13.292734760878805	10.623048850195664
42	20.001392369813423	53.021442495126706	14.581776077620953	12.39538905743892
43	19.88194169634613	50.979055826701256	16.389291943308564	12.749710533644052
44	21.52200677131425	45.95773058376936	20.602309868237846	11.917952776678538
45	22.800788521010126	45.79577598968181	17.97989124858931	13.423544240718757
46	25.04470239927304	47.190710694865814	14.79539492004866	12.969191985812484
47	27.23218865878146	42.93005906579314	14.933166248955724	14.904586026469682
48	27.15047853552009	44.11357340720222	15.556068533907869	13.179879523369827
49	26.18094944964751	43.468686335722346	14.413592460683875	15.93677175394627
50	24.763297131718183	45.83498219232291	15.283823594072901	14.117897081886003
51	23.54350789253836	45.20328599275968	16.863064092980988	14.390142021720969
52	23.13056031892597	45.71223380087646	15.551671576602324	15.605534303595245
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	0.5
4	0.0
5	1.0
6	2.0
7	81.0
8	160.0
9	224.0
10	288.0
11	464.5
12	641.0
13	811.5
14	1187.5
15	1393.0
16	1852.5
17	2312.0
18	3241.0
19	4170.0
20	5939.5
21	7709.0
22	10224.0
23	12739.0
24	15650.0
25	18561.0
26	18747.0
27	18933.0
28	17853.5
29	16774.0
30	15448.5
31	14123.0
32	14151.5
33	14180.0
34	13191.5
35	12203.0
36	11534.0
37	10865.0
38	11572.0
39	12739.0
40	13199.0
41	14972.5
42	16746.0
43	18091.5
44	19437.0
45	19718.0
46	19999.0
47	18307.5
48	16616.0
49	14918.0
50	13220.0
51	11538.0
52	9856.0
53	7513.0
54	5170.0
55	4278.5
56	3387.0
57	3233.5
58	3080.0
59	2261.0
60	1442.0
61	1287.5
62	1133.0
63	910.0
64	486.5
65	286.0
66	224.0
67	162.0
68	120.5
69	79.0
70	65.5
71	52.0
72	33.5
73	15.0
74	10.5
75	6.0
76	6.0
77	6.0
78	3.0
79	0.0
80	5.5
81	11.0
82	10.0
83	9.0
84	6.0
85	3.0
86	1.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	272916.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.45920392588513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.9604297552103	47.672970033509316
2	5.878490881821994	6.5203284918284465
3	2.2132328834754715	3.6823240146042284
4	1.3457205145162197	2.9853035376720833
5	0.8707294907598019	2.414498219616498
6	0.6242278224049597	2.0771506859380713
7	0.48674666001729416	1.8896207594699812
8	0.36348587432968527	1.6126909782902936
9	0.30260827315746663	1.5104172537629903
>10	1.804715640408993	17.549423026333688
>50	0.07347027869983433	2.8885232282842597
>100	0.07347027869983433	7.831016872166906
>500	0.0020037348736318453	0.8971535340990608
>1k	6.679116245439485E-4	0.46857936442415854
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACGGAATTAGGGCAATTGCAGGGAGAGGGGGCACCCCCAGACTACCAGGAA	1265	0.463512582626156	No Hit
TGATGAATTAGGGACGCAGAGTGCTTGAATTAGGGCTTTTGTTGCAGACGAC	951	0.3484588664644066	No Hit
TATGGAATTAGGGCTTAGATACCATAGAGAATTGCACTGGAAGTAAAATATA	936	0.3429626698324759	No Hit
GTTGGAATTAGGGGGGCAGACTGTCTGGACAAAGTCTGTGGTTTAGCCAAGG	535	0.19603101320552846	No Hit
CTACGAATTAGGGATCCACATGAGTCAGCAATGCTTTGTTATCCATCTCGAC	470	0.17221416113382873	No Hit
ATAAGAATTAGGGCCGTGTCTACTAAAAATACAAAAAAAAATTTAGCCGGGT	440	0.1612217678699673	No Hit
GTTCGAATTAGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG	425	0.1557255712380366	No Hit
TGGCGAATTAGGGACAATCTGGTATTCTTCATGCAGCCGATGAAAGAGATTT	413	0.15132861393249206	No Hit
TGTAGAATTAGGGGGCACTCAAAGTATGCCTATTCCAGAGACACCAGACATC	412	0.15096220082369666	No Hit
GGTTGAATTAGGGAGAATCACCTCGCCAACCACATCTGCTTTATGGGGGAAA	403	0.14766448284453823	No Hit
ACCTGAATTAGGGTGTTTTTACCCACCAACATTCTTCTCTGTTCCTTGAGGT	379	0.13887056823344912	No Hit
TTTAGAATTAGGGTGGATCACCTGAGGTTGGAAGTTGAAGACCAGCCTGGCC	377	0.13813774201585835	No Hit
TTTGGAATTAGGGGAACCTGCCCGGGACCGCTGGCGGCCCCAACTGGATTTG	364	0.1333743716015184	No Hit
TGGGGAATTAGGGACCCAGCCACACCAGCTTGACTAAGGTGTTTGAGCCATT	349	0.1278781749695877	No Hit
TTAGGAATTAGGGGGCACATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGG	331	0.12128273901127087	No Hit
GGTCGAATTAGGGGAAGTCCAAGTTTATCCATCGTACCTGGTCATTCTCATT	311	0.11395447683536326	No Hit
CGTTGAATTAGGGGTGTCCGTTGTCGTCTGGGCTCGGGTAGATCGATGCACA	303	0.11102317196500022	No Hit
TATTGAATTAGGGCACCATCTTCACAAAGCCCTAAAAATGAAGAATAACAAC	296	0.10845828020343257	No Hit
GGGTGAATTAGGGGGAGTAGTCGAATTAGGGGTTTTGAATCGTCGGGCCATC	291	0.10662621465945565	No Hit
AGCAGAATTAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC	290	0.10625980155066028	No Hit
GAGTGAATTAGGGCACTTTCACTGAATAACATAACTCAAATTAGTTCTGTGA	290	0.10625980155066028	No Hit
GTTTGAATTAGGGTGGTGGCGGGCACCTGTAATCCCAGCTACTTGGGAGGCT	286	0.10479414911547875	No Hit
GTTCGAATTAGGGAGCCGTTCGGCCGAATGACTCGGTGTCGCATCGCGGCTT	280	0.10259567046270648	No Hit
CATGGAATTAGGGTGCCAGGGATGTGATAGACACTAAAGATGCATTCGTTAA	273	0.10003077870113881	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.16195459408755808	0.0	0.0	0.0	0.0
11	0.1802752495273271	0.0	0.0	0.0	0.0
12	0.19419894766155155	0.0	0.0	0.0	0.0
13	0.2956953787978719	0.0	0.0	0.0	0.0
14	0.6778642512714534	0.0	0.0	0.0	0.0
15	1.131117266851339	0.0	0.0	0.0	0.0
16	1.3212856703161413	0.0	0.0	0.0	0.0
17	1.492400592123584	0.0	0.0	0.0	0.0
18	1.7338668308197394	0.0	0.0	0.0	0.0
19	1.9559131747497398	0.0	0.0	0.0	0.0
20	2.186387020182034	0.0	0.0	0.0	0.0
21	2.4135631476351698	0.0	0.0	0.0	0.0
22	2.6616248222896424	0.0	0.0	0.0	0.0
23	2.889533775960369	0.0	0.0	0.0	0.0
24	3.1291679491125475	0.0	0.0	0.0	0.0
25	3.402512128273901	0.0	0.0	0.0	0.0
26	3.7462076243239677	0.0	0.0	0.0	0.0
27	4.1148192117721205	0.0	0.0	0.0	0.0
28	4.475003297717979	0.0	0.0	0.0	0.0
29	4.879156956719283	0.0	0.0	0.0	0.0
30	5.362089434111595	0.0	0.0	0.0	0.0
31	5.770640050418444	0.0	0.0	0.0	0.0
32	6.126060765949963	0.0	0.0	0.0	0.0
33	6.4129622301367455	0.0	0.0	0.0	0.0
34	6.665787275205558	0.0	0.0	0.0	0.0
35	6.886734379809172	0.0	0.0	0.0	0.0
36	7.102918113998446	0.0	0.0	0.0	0.0
37	7.308475868032655	0.0	0.0	0.0	0.0
38	7.533087103724223	0.0	0.0	0.0	0.0
39	7.7540342083278375	0.0	0.0	0.0	0.0
40	7.990370663500857	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTTTA	55	1.8189894E-12	46.000004	39
TTCACTG	55	1.8189894E-12	46.000004	18
TGTGAAC	35	1.0157419E-7	46.000004	23
ACTGTCA	35	1.0157419E-7	46.000004	22
TTGTTAT	75	0.0	46.000004	36
CCTACTC	35	1.0157419E-7	46.000004	29
CCGTGCC	55	1.8189894E-12	46.000004	35
CGATACC	35	1.0157419E-7	46.000004	43
ACTTGTG	35	1.0157419E-7	46.000004	39
GAAATGA	35	1.0157419E-7	46.000004	27
CGCCTGA	35	1.0157419E-7	46.000004	15
GATACCG	35	1.0157419E-7	46.000004	44
AACGAAG	35	1.0157419E-7	46.000004	29
CAACTTT	55	1.8189894E-12	46.000004	16
GCCTACC	55	1.8189894E-12	46.000004	39
TTCGATG	55	1.8189894E-12	46.000004	21
GACGGGT	35	1.0157419E-7	46.000004	42
GGATTTT	35	1.0157419E-7	46.000004	45
CATTCTC	35	1.0157419E-7	46.000004	43
CACTGTC	35	1.0157419E-7	46.000004	21
>>END_MODULE