FastQCFastQC Report
Fri 17 Jun 2016
SRR1528048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285929
Sequences flagged as poor quality0
Sequence length59
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAATTAGGGCAATTGCAGGGAGAGGGGGCACCCCCAGACTACCAGGAAGGGTGGA14630.511665483389233No Hit
TATGGAATTAGGGCTTAGATACCATAGAGAATTGCACTGGAAGTAAAATATATGTACCT11320.39590247928681593No Hit
TGATGAATTAGGGACGCAGAGTGCTTGAATTAGGGCTTTTGTTGCAGACGACGAATTGC9150.3200095128510924No Hit
CTACGAATTAGGGATCCACATGAGTCAGCAATGCTTTGTTATCCATCTCGACCAGATAA5180.18116385536269494No Hit
ATAAGAATTAGGGCCGTGTCTACTAAAAATACAAAAAAAAATTTAGCCGGGTGTGGTGG4840.16927279149718985No Hit
GTTGGAATTAGGGGGGCAGACTGTCTGGACAAAGTCTGTGGTTTAGCCAAGGTGGTCAG4740.16577541977204133No Hit
TGTAGAATTAGGGGGCACTCAAAGTATGCCTATTCCAGAGACACCAGACATCACGAAAG4460.15598277894162538No Hit
TGGCGAATTAGGGACAATCTGGTATTCTTCATGCAGCCGATGAAAGAGATTTTAAAACT4410.1542340930790511No Hit
ACCTGAATTAGGGTGTTTTTACCCACCAACATTCTTCTCTGTTCCTTGAGGTCCTCCTC4380.15318488156150653No Hit
TGGGGAATTAGGGACCCAGCCACACCAGCTTGACTAAGGTGTTTGAGCCATTGCATGTG3900.13639749728079348No Hit
GGTCGAATTAGGGGAAGTCCAAGTTTATCCATCGTACCTGGTCATTCTCATTACCTTTC3900.13639749728079348No Hit
TTTAGAATTAGGGTGGATCACCTGAGGTTGGAAGTTGAAGACCAGCCTGGCCAAAAGGG3880.13569802293576377No Hit
TTAGGAATTAGGGGGCACATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGA3760.13150117686558552No Hit
GGTTGAATTAGGGAGAATCACCTCGCCAACCACATCTGCTTTATGGGGGAAAAGCTGGC3480.12170853603516957No Hit
TTTGGAATTAGGGGAACCTGCCCGGGACCGCTGGCGGCCCCAACTGGATTTGAAGTCCA3310.11576300410241704No Hit
GAGTGAATTAGGGCACTTTCACTGAATAACATAACTCAAATTAGTTCTGTGAGAATTTA3250.11366458106732791No Hit
TATTGAATTAGGGCACCATCTTCACAAAGCCCTAAAAATGAAGAATAACAACAGCCCTG3230.11296510672229819No Hit
GTTTGAATTAGGGTGGTGGCGGGCACCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAG3230.11296510672229819No Hit
AGCAGAATTAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCAAGAA3110.10876826065211993No Hit
CGTTGAATTAGGGGTGTCCGTTGTCGTCTGGGCTCGGGTAGATCGATGCACATGACCGT3080.10771904913457536No Hit
CCTGGAATTAGGGGGATTTAAAATGCGACATGCATGCCCAGGGAAAACACTTGCCCGGA3050.1066698376170308No Hit
TTCAGAATTAGGGAATCCTTTGCTTTGGATAGGAATTATTGCTTCAATTTACATTATTT2980.10422167740942681No Hit
GAGGGAATTAGGGAACTGTGGATCGATATCCTGGCTCAGACGACGGGTCATACCCATGA2960.1035222030643971No Hit
GTGCGAATTAGGGATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGG2860.10002483133924855No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAT150.006691146653.026
ATCCTTT306.991777E-753.015
GTCATTT251.4759133E-553.025
TATCATA150.006691146653.049
CTTAAGA150.006691146653.018
GTGTTCA503.6379788E-1253.013
TTGATAC401.5934347E-953.038
GCCCCAT203.1342515E-453.016
ATCCTAG203.1342515E-453.032
TTAGGTT251.4759133E-553.08
GTTTGAC203.1342515E-453.022
CGGGTCA203.1342515E-453.044
GGAATGT150.006691146653.012
ACTATCG150.006691146653.043
TGGAGAC251.4759133E-553.053
TCTGACC150.006691146653.040
TCTGACA203.1342515E-453.041
ATCTCGA850.053.045
TTGGCCC251.4759133E-553.018
CTGTCAT150.006691146653.023
Produced by FastQC (version 0.11.5)