FastQCFastQC Report
Fri 17 Jun 2016
SRR1528047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926945
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCTGGATGGGGGTGAATGGCCACCGGGGCCACCGTTATGCAATGTTCAGC32440.16834938205293873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA351.0200529E-746.00000433
TTTACGG351.0200529E-746.00000415
ATAGCGA351.0200529E-746.00000429
CGTTAGC750.046.00000430
CTAAAGG351.0200529E-746.00000443
TCATCGA351.0200529E-746.00000437
CGGTAAT700.046.00000428
CGTTTAA351.0200529E-746.00000434
ATCGCAT700.046.00000444
TAATCAT206.312522E-446.025
TATTTCG206.312522E-446.041
ACAACGG405.6152203E-946.038
TTTAGGC301.8620631E-646.044
CGGCGTA301.8620631E-646.033
CTATGCG501.6370905E-1146.017
TGATACT1900.046.032
TCGTTAA253.4180237E-546.034
CGACGTT206.312522E-446.031
CGACGTA206.312522E-446.036
ACGTTAG206.312522E-446.033
Produced by FastQC (version 0.11.5)