##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528047_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1926945
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.645044876734936	31.0	31.0	34.0	30.0	34.0
2	32.24519277924383	33.0	31.0	34.0	30.0	34.0
3	32.41937522866506	34.0	31.0	34.0	30.0	34.0
4	36.033713468729	37.0	35.0	37.0	35.0	37.0
5	35.696772352091	37.0	35.0	37.0	33.0	37.0
6	35.909121433149366	37.0	35.0	37.0	35.0	37.0
7	35.87200932045284	37.0	35.0	37.0	35.0	37.0
8	35.42253048218813	37.0	35.0	37.0	33.0	37.0
9	37.819706841658686	39.0	38.0	39.0	35.0	39.0
10	37.677114292312446	39.0	37.0	39.0	35.0	39.0
11	38.170127844852864	39.0	39.0	39.0	37.0	39.0
12	38.22773509363267	39.0	39.0	39.0	37.0	39.0
13	38.09952230084408	39.0	39.0	39.0	37.0	39.0
14	38.47852896683611	40.0	38.0	40.0	35.0	40.0
15	36.90120371883992	39.0	35.0	40.0	32.0	40.0
16	36.53404482224454	39.0	35.0	40.0	31.0	40.0
17	36.557342321654225	39.0	35.0	40.0	31.0	40.0
18	36.76161177407762	39.0	35.0	40.0	31.0	40.0
19	36.96659531019308	39.0	35.0	40.0	32.0	40.0
20	36.98955756391594	39.0	35.0	40.0	32.0	40.0
21	36.83880494772814	39.0	35.0	40.0	32.0	40.0
22	36.876661243574674	39.0	35.0	40.0	32.0	40.0
23	36.77141537511449	39.0	35.0	40.0	31.0	40.0
24	36.75461728279738	39.0	35.0	40.0	31.0	40.0
25	36.94858234147835	39.0	35.0	40.0	32.0	40.0
26	36.997988525879045	39.0	35.0	40.0	32.0	40.0
27	36.956612669277014	39.0	35.0	40.0	32.0	40.0
28	36.903430040815905	39.0	35.0	40.0	32.0	40.0
29	36.886088082431	39.0	35.0	40.0	32.0	40.0
30	36.810750696049965	39.0	35.0	40.0	32.0	40.0
31	36.76245352098788	39.0	35.0	40.0	32.0	40.0
32	36.64968175012779	39.0	35.0	40.0	32.0	40.0
33	36.54629426371796	39.0	35.0	40.0	31.0	40.0
34	36.46071320146657	39.0	35.0	40.0	31.0	40.0
35	36.35966880217131	39.0	35.0	40.0	31.0	40.0
36	36.25278977863925	38.0	35.0	40.0	31.0	40.0
37	36.09951814919471	38.0	35.0	40.0	31.0	40.0
38	35.894065995656334	38.0	35.0	40.0	30.0	40.0
39	35.7323358995716	38.0	35.0	40.0	30.0	40.0
40	35.51974290911261	37.0	35.0	40.0	30.0	40.0
41	35.31413247394191	37.0	35.0	40.0	29.0	40.0
42	35.09983523141553	37.0	34.0	40.0	28.0	40.0
43	34.861071281224945	36.0	34.0	40.0	27.0	40.0
44	34.69322632457076	36.0	34.0	40.0	27.0	40.0
45	34.52433048166917	35.0	34.0	40.0	26.0	40.0
46	34.355429449205865	35.0	34.0	40.0	26.0	40.0
47	34.20715796247428	35.0	34.0	39.0	26.0	40.0
48	34.04672318099375	35.0	33.0	39.0	25.0	40.0
49	33.88723964617568	35.0	33.0	39.0	25.0	40.0
50	33.697232147259	35.0	33.0	39.0	24.0	40.0
51	33.519834764354975	35.0	33.0	39.0	24.0	40.0
52	32.83511672621689	35.0	33.0	37.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	2.0
14	7.0
15	17.0
16	39.0
17	160.0
18	380.0
19	830.0
20	1653.0
21	2714.0
22	4404.0
23	6728.0
24	9661.0
25	13248.0
26	17823.0
27	23404.0
28	29918.0
29	37786.0
30	46531.0
31	54186.0
32	64453.0
33	82925.0
34	144848.0
35	237297.0
36	168172.0
37	305855.0
38	474408.0
39	199493.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.675494111144843	20.058745838620197	34.94567826274232	16.320081787492636
2	22.060152209845118	22.036591599656454	35.495045265952065	20.408210924546367
3	19.539530188977892	23.826575226589238	35.264836308249585	21.369058276183285
4	19.790756871628407	22.282213555654156	37.34963893624364	20.577390636473798
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	99.05876919164793	0.6196336688384982	0.2377857178072026	0.08381142170637979
12	98.58236742615902	1.0168427225478673	0.12537981104805793	0.2754100402450511
13	93.38958818233006	3.3392234858805	1.796055414139999	1.4751329176494399
14	54.569798307683925	27.1209090036301	7.6216498135649955	10.68764287512098
15	32.50808922932414	30.358780349205606	17.18440329122004	19.948727130250216
16	28.57917584570395	21.471240746362767	25.90105062676932	24.048532781163967
17	22.14152453754518	21.38332957090109	28.25311568311498	28.22203020843875
18	23.337510930514362	21.82532454221579	29.531979376681743	25.305185150588105
19	25.738098388900564	21.353333904185124	27.239179115127833	25.66938859178648
20	27.488797033646524	20.793847255630027	24.71502819229402	27.002327518429432
21	31.338206331784253	21.79787176074045	19.800565143270827	27.063356764204478
22	29.573392079171956	22.14105747699078	21.849196526107388	26.43635391772988
23	28.86382330580271	23.348357114499898	20.54651274426618	27.24130683543121
24	29.75118646354722	24.071211165860987	21.319601753033947	24.858000617557842
25	31.77537501070347	22.08200026466765	21.16827413340806	24.97435059122082
26	30.41534657190527	22.78752117989875	20.45782313454717	26.339309113648806
27	29.844390991958775	24.45150224837761	19.776952637465005	25.92715412219861
28	30.125405758856637	23.483752779658996	20.6683117577305	25.72252970375387
29	30.947172856516403	22.298923944378277	20.02558453925774	26.728318659847584
30	30.2026783328014	22.238309863540476	20.130309894677843	27.42870190898028
31	30.234853615437906	22.732148556393668	19.430082332396616	27.602915495771807
32	30.153118018417757	24.4972741827089	20.016191432552564	25.333416366320783
33	30.62785912415767	24.694996484071936	19.998702609571108	24.678441782199283
34	27.862445477167224	26.181494541878465	19.769998624766146	26.186061356188162
35	30.13121806797807	25.84318701364076	19.779080357768386	24.246514560612784
36	31.71828983183225	24.41273622236234	20.079711667951084	23.789262277854323
37	30.35078842416364	25.540739356857618	19.679388877212375	24.429083341766372
38	30.03188985674215	26.509941902856593	21.3118174104606	22.14635082994066
39	31.08381401648724	26.213462242046347	20.624148587531042	22.078575153935372
40	30.752823770268485	26.4544654881172	20.207790051091234	22.584920690523084
41	32.02784718816572	25.682621974161172	20.076961200241833	22.21256963743127
42	32.655939842600596	26.01392359408286	18.25397196079805	23.076164602518496
43	31.030413426434073	25.785738565449456	19.16967012550955	24.014177882606923
44	29.61828178801159	26.330590649966656	21.115703873229386	22.935423688792365
45	28.661897459450064	23.982365869290508	20.960172708613893	26.395563962645536
46	29.068707202333226	22.87517287727465	21.458474424542477	26.597645495849648
47	28.507975058966394	23.124531317707564	22.901535850789724	25.465957772536317
48	27.918440848078173	23.936074978787666	22.13976008656189	26.00572408657227
49	28.642280916165223	22.942585283959843	22.69649626740774	25.7186375324672
50	27.799288511088793	23.998868675546007	21.058774381209634	27.143068432155566
51	28.4267584181178	24.869521444566402	22.092586970567403	24.611133166748402
52	29.527049293051956	24.434065321013314	22.15299346893658	23.88589191699815
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	2.5
8	3.0
9	57.0
10	111.0
11	358.0
12	605.0
13	1043.5
14	1812.0
15	2142.0
16	2146.5
17	2151.0
18	2376.5
19	2602.0
20	3117.5
21	3633.0
22	4778.5
23	5924.0
24	7515.5
25	9107.0
26	11340.5
27	13574.0
28	15309.5
29	17045.0
30	18748.0
31	20451.0
32	20677.0
33	20903.0
34	22436.5
35	23970.0
36	25858.0
37	27746.0
38	29372.0
39	34329.0
40	37660.0
41	39786.5
42	41913.0
43	43858.5
44	45804.0
45	50071.5
46	54339.0
47	60203.0
48	66067.0
49	75673.5
50	85280.0
51	90765.0
52	96250.0
53	108125.0
54	120000.0
55	132694.0
56	145388.0
57	154863.5
58	164339.0
59	167128.5
60	169918.0
61	177623.0
62	185328.0
63	164998.5
64	133651.5
65	122634.0
66	105404.0
67	88174.0
68	75395.0
69	62616.0
70	54296.0
71	45976.0
72	40457.0
73	34938.0
74	26552.0
75	18166.0
76	13670.0
77	9174.0
78	6333.0
79	3492.0
80	2399.5
81	1307.0
82	904.5
83	502.0
84	475.5
85	449.0
86	255.5
87	62.0
88	55.5
89	25.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1926945.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.051412618039773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.45444976006365	9.454869457797985
2	6.918440828061378	1.6675397018492084
3	3.232434581947198	1.1686620872359974
4	2.0099667301057234	0.9689175365214504
5	1.305781299086239	0.7868254612104133
6	0.9300623144121837	0.672513882688216
7	0.6940535061182609	0.5855027626859848
8	0.4816130405639351	0.4643293979251767
9	0.40615486981560245	0.4405265930676648
>10	2.5389015801497195	5.9492759903655275
>50	0.6268404399211157	5.607564294992364
>100	2.2588766680406756	60.54526062292497
>500	0.13290056287629118	10.154394559284798
>1k	0.00952381883804041	1.5338176514502462
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCCTGGATGGGGGTGAATGGCCACCGGGGCCACCGTTATGCAATGTTCAGC	3244	0.16834938205293873	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.003477006349428759	0.0	0.0	0.0	0.0
12	0.012870113054601973	0.0	0.0	0.0	0.0
13	0.027660363943963113	0.0	0.0	0.0	0.0
14	0.09284125909146343	0.0	0.0	0.0	0.0
15	0.1572956155987846	0.0	0.0	0.0	0.0
16	0.18998985440684607	0.0	0.0	0.0	0.0
17	0.21095568373772994	0.0	0.0	0.0	0.0
18	0.23519093694941995	0.0	0.0	0.0	0.0
19	0.2586996515209308	0.0	0.0	0.0	0.0
20	0.2848031469502243	0.0	0.0	0.0	0.0
21	0.31355331885445614	0.0	0.0	0.0	0.0
22	0.3489980253717672	0.0	0.0	0.0	0.0
23	0.3841313581861444	0.0	0.0	0.0	0.0
24	0.42590732999644515	0.0	0.0	0.0	0.0
25	0.4721982204992877	0.0	0.0	0.0	0.0
26	0.5206687269226677	0.0	0.0	0.0	0.0
27	0.5686202771744913	0.0	0.0	0.0	0.0
28	0.6149630632944895	0.0	0.0	0.0	0.0
29	0.668000384027567	0.0	0.0	0.0	0.0
30	0.7245147111100732	0.0	0.0	0.0	0.0
31	0.7779671967803959	0.0	0.0	0.0	0.0
32	0.8215595151911446	0.0	0.0	0.0	0.0
33	0.8611558710809079	0.0	0.0	0.0	0.0
34	0.9020496173995625	0.0	0.0	0.0	0.0
35	0.9355222904649588	0.0	0.0	0.0	0.0
36	0.9667115563755063	0.0	0.0	0.0	0.0
37	0.9983678828404547	0.0	0.0	0.0	0.0
38	1.0322557208430962	0.0	0.0	0.0	0.0
39	1.0715407030299255	0.0	0.0	0.0	0.0
40	1.1143545871833394	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	35	1.0200529E-7	46.000004	33
TTTACGG	35	1.0200529E-7	46.000004	15
ATAGCGA	35	1.0200529E-7	46.000004	29
CGTTAGC	75	0.0	46.000004	30
CTAAAGG	35	1.0200529E-7	46.000004	43
TCATCGA	35	1.0200529E-7	46.000004	37
CGGTAAT	70	0.0	46.000004	28
CGTTTAA	35	1.0200529E-7	46.000004	34
ATCGCAT	70	0.0	46.000004	44
TAATCAT	20	6.312522E-4	46.0	25
TATTTCG	20	6.312522E-4	46.0	41
ACAACGG	40	5.6152203E-9	46.0	38
TTTAGGC	30	1.8620631E-6	46.0	44
CGGCGTA	30	1.8620631E-6	46.0	33
CTATGCG	50	1.6370905E-11	46.0	17
TGATACT	190	0.0	46.0	32
TCGTTAA	25	3.4180237E-5	46.0	34
CGACGTT	20	6.312522E-4	46.0	31
CGACGTA	20	6.312522E-4	46.0	36
ACGTTAG	20	6.312522E-4	46.0	33
>>END_MODULE