FastQCFastQC Report
Fri 17 Jun 2016
SRR1528046_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528046_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911021
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCTGGATGGGGGTGAATGGCCACCGGGGCCACCGTTATGCAATGTTCAGC30980.16211229494600007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAAG750.046.00000433
ACAACGG501.6370905E-1146.038
AACGTTA206.31251E-446.036
GATACCT650.046.016
TCGTTAG253.418012E-546.033
TCGCAAT501.6370905E-1146.026
CTCGTTA253.418012E-546.032
GTATTCG501.6370905E-1146.030
CGAAATA206.31251E-446.022
CGACGTA206.31251E-446.036
ACGTTAT301.8620558E-646.029
CCGATAT551.8189894E-1246.032
ATAATTC206.31251E-446.013
GACGTTA206.31251E-446.014
CTATACC206.31251E-446.021
CCAATTG253.418012E-546.029
ACCGATA501.6370905E-1146.031
TATATCG206.31251E-446.017
CTAAGTA301.8620558E-646.032
TACGGTA253.418012E-546.032
Produced by FastQC (version 0.11.5)