##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528046_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1911021
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.586803598704567	31.0	31.0	34.0	30.0	34.0
2	32.078288516976	33.0	31.0	34.0	30.0	34.0
3	32.34514691361319	34.0	31.0	34.0	30.0	34.0
4	36.00510774083592	37.0	35.0	37.0	35.0	37.0
5	35.677921383386156	37.0	35.0	37.0	33.0	37.0
6	35.89627691166136	37.0	35.0	37.0	35.0	37.0
7	35.86886120037404	37.0	35.0	37.0	35.0	37.0
8	35.37598487928704	37.0	35.0	37.0	33.0	37.0
9	37.807306670099386	39.0	37.0	39.0	35.0	39.0
10	37.66695865717855	39.0	37.0	39.0	35.0	39.0
11	38.15616311908661	39.0	39.0	39.0	37.0	39.0
12	38.21031584686929	39.0	39.0	39.0	37.0	39.0
13	38.10457289584991	39.0	39.0	39.0	37.0	39.0
14	38.510432904714285	40.0	39.0	40.0	36.0	40.0
15	36.936058264142574	39.0	35.0	40.0	32.0	40.0
16	36.5760318698748	39.0	35.0	40.0	31.0	40.0
17	36.616128760489815	39.0	35.0	40.0	31.0	40.0
18	36.840102228075985	39.0	35.0	40.0	32.0	40.0
19	37.03362129458546	39.0	35.0	40.0	32.0	40.0
20	37.03788655383693	39.0	35.0	40.0	32.0	40.0
21	37.07295157928667	39.0	35.0	40.0	32.0	40.0
22	37.0146900531182	39.0	35.0	40.0	32.0	40.0
23	36.886001252733486	39.0	35.0	40.0	32.0	40.0
24	36.86443738713494	39.0	35.0	40.0	32.0	40.0
25	36.76931389032355	39.0	35.0	40.0	31.0	40.0
26	36.89229317731202	39.0	35.0	40.0	32.0	40.0
27	36.89156477087379	39.0	35.0	40.0	32.0	40.0
28	36.699000691253524	39.0	35.0	40.0	32.0	40.0
29	36.790490005081054	39.0	35.0	40.0	32.0	40.0
30	36.788522470449045	39.0	35.0	40.0	32.0	40.0
31	36.77468431796406	39.0	35.0	40.0	32.0	40.0
32	36.66412927958405	39.0	35.0	40.0	32.0	40.0
33	36.55242878021748	39.0	35.0	40.0	31.0	40.0
34	36.49765858145986	39.0	35.0	40.0	31.0	40.0
35	36.330652567397216	39.0	35.0	40.0	31.0	40.0
36	35.842425593439316	38.0	35.0	40.0	30.0	40.0
37	35.90667030869886	38.0	35.0	40.0	30.0	40.0
38	35.8207251516336	38.0	35.0	40.0	30.0	40.0
39	35.69532045958679	38.0	35.0	40.0	30.0	40.0
40	35.48489995662005	37.0	35.0	40.0	29.0	40.0
41	35.31736909222871	37.0	35.0	40.0	29.0	40.0
42	34.96595955774426	37.0	34.0	40.0	28.0	40.0
43	34.726523675040724	36.0	34.0	40.0	27.0	40.0
44	34.56871012929737	36.0	34.0	40.0	26.0	40.0
45	34.174396827664374	35.0	34.0	40.0	24.0	40.0
46	34.15304436738267	35.0	33.0	40.0	24.0	40.0
47	34.08190438514281	35.0	33.0	39.0	25.0	40.0
48	33.91705219356564	35.0	33.0	39.0	24.0	40.0
49	33.77277957699052	35.0	33.0	39.0	24.0	40.0
50	33.51272173356546	35.0	33.0	39.0	23.0	40.0
51	33.422642660651036	35.0	33.0	39.0	23.0	40.0
52	32.88552297436815	35.0	33.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	4.0
14	7.0
15	20.0
16	50.0
17	164.0
18	410.0
19	791.0
20	1546.0
21	2791.0
22	4404.0
23	6634.0
24	9914.0
25	13468.0
26	18282.0
27	23738.0
28	30516.0
29	38235.0
30	46808.0
31	54746.0
32	64866.0
33	85218.0
34	145780.0
35	234062.0
36	170116.0
37	306250.0
38	457249.0
39	194950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.750024201722535	20.087952984294784	34.77758747810725	16.38443533587543
2	22.139421806458433	22.07092439067912	35.36784786771051	20.421805935151944
3	19.557974506821225	23.766876449814	35.32875881531391	21.34639022805087
4	19.853994278451154	22.27003261607277	37.29519455830156	20.58077854717452
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	99.05799046687608	0.6175756310370216	0.24541854851411887	0.07901535357277602
12	98.55867622595461	1.0337929305852736	0.1322853071734952	0.27524553628662374
13	93.36569299866406	3.3617631622049156	1.802387310238872	1.4701565288921472
14	54.52075094936163	27.10456870960602	7.598241986875079	10.77643835415728
15	32.57264048903701	30.226146128169184	17.196618980115865	20.00459440267794
16	28.59126090189485	21.440005107217555	25.95790417792374	24.010829812963856
17	22.156637734488527	21.366065574370978	28.28085091686591	28.196445774274586
18	23.342914599054644	21.738955249576012	29.627879547111203	25.29025060425814
19	25.843096439023956	21.273758896422386	27.224399941183275	25.658744723370386
20	27.575416492021805	20.732163592132164	24.67126211590558	27.021157799940447
21	31.015828711458433	21.95412818592784	19.79659040900126	27.23345269361247
22	29.554149326459523	22.177621282026728	21.82650007509075	26.441729316423
23	28.87336141256428	23.309006023481686	20.544253569165384	27.27337899478865
24	29.69156278240794	24.076553842160813	21.283125617143924	24.948757758287325
25	31.788295366717584	22.09557090162798	21.127972952678174	24.988160778976265
26	30.424312448685807	22.80916850207298	20.423532760759823	26.34298628848139
27	29.898049262671627	24.481572939282195	19.711871298117604	25.908506499928574
28	30.218035280617013	23.482682817195624	20.70558094338053	25.593700958806835
29	30.978728124913328	22.31058685383363	19.96817408076625	26.742510940486785
30	30.168532946524394	22.27468981240918	20.12091965499071	27.43585758607572
31	30.22112263549171	22.7239784387508	19.38602453871517	27.66887438704232
32	30.10746611366385	24.57461220991292	20.064353034320398	25.253568642102834
33	30.70039523375201	24.638400101307102	20.024950013631457	24.63625465130943
34	27.914816216043675	26.07710747291631	19.778484904142864	26.22959140689715
35	30.207203374531204	25.79710008419583	19.76896119927515	24.226735341997813
36	31.798342352072527	24.230869257846983	20.20893543294396	23.761852957136526
37	30.457593087674073	25.507359678412744	19.589475992152884	24.445571241760295
38	30.015159435715255	26.514726944392553	21.302800963464033	22.167312656428162
39	31.102169991852524	26.185635845969244	20.576696959374072	22.135497202804157
40	30.788881964143776	26.423676139613328	20.229029403653858	22.55841249258904
41	31.952029831174016	25.700816474544236	20.01788572705376	22.329267967227988
42	32.79377882294334	25.942258091355352	18.252180378970195	23.01178270673111
43	31.053766546783105	25.793908073223687	19.16043832066733	23.99188705932588
44	29.821336343242695	26.23576611664655	21.030276485711042	22.912621054399715
45	28.671636784734446	24.002980605655303	21.00856034549071	26.316822264119548
46	29.095912603786147	22.829105488636703	21.51446792055137	26.560513987025786
47	28.491575969076216	23.11874123832234	22.867409620302446	25.522273172299
48	27.925020185544795	23.937884513043027	22.118752227212575	26.018343074199603
49	28.721400759070676	22.8882885117432	22.688813990008484	25.701496739177642
50	27.817486045417606	23.976345628854943	21.17182385750863	27.034344468218823
51	28.387076855775	24.80940816453613	22.19012768567169	24.613387294017176
52	29.515478898452713	24.495910824632485	22.14606746864634	23.842542808268462
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	51.5
10	100.0
11	347.0
12	594.0
13	1022.5
14	1755.5
15	2060.0
16	2068.0
17	2076.0
18	2300.0
19	2524.0
20	3048.5
21	3573.0
22	4700.0
23	5827.0
24	7360.5
25	8894.0
26	11054.0
27	13214.0
28	15034.5
29	16855.0
30	18426.0
31	19997.0
32	20203.5
33	20410.0
34	22141.0
35	23872.0
36	25720.5
37	27569.0
38	29043.5
39	33896.5
40	37275.0
41	39471.0
42	41667.0
43	43801.0
44	45935.0
45	49992.0
46	54049.0
47	59895.0
48	65741.0
49	75117.0
50	84493.0
51	90384.0
52	96275.0
53	107695.0
54	119115.0
55	131583.0
56	144051.0
57	153210.5
58	162370.0
59	165157.0
60	167944.0
61	175522.5
62	183101.0
63	163457.5
64	132929.5
65	122045.0
66	104879.5
67	87714.0
68	75111.0
69	62508.0
70	54221.0
71	45934.0
72	40226.0
73	34518.0
74	26275.5
75	18033.0
76	13509.5
77	8986.0
78	6259.5
79	3533.0
80	2392.5
81	1252.0
82	884.5
83	517.0
84	511.0
85	505.0
86	284.5
87	64.0
88	52.5
89	22.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1911021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.886887739440981
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.55013104853893	10.122667207408986
2	7.197807640125589	1.8551467805677815
3	3.3478680897232502	1.2943080071616073
4	1.9399951158822852	1.0000199709375523
5	1.3291920336429208	0.8564574260857789
6	0.892834270908731	0.6903513011475871
7	0.6404502266761768	0.5777387121712793
8	0.4731556145135987	0.48780026300183665
9	0.3903000597827596	0.45267777496057743
>10	2.3309768464123577	5.907921622741512
>50	0.6288072484902232	6.040471526702413
>100	2.1584291530709487	60.30263878454451
>500	0.11165736186636958	9.000581161022767
>1k	0.00839529036589245	1.411219461545815
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCCTGGATGGGGGTGAATGGCCACCGGGGCCACCGTTATGCAATGTTCAGC	3098	0.16211229494600007	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0033489951183163346	0.0	0.0	0.0	0.0
12	0.010308625598567467	0.0	0.0	0.0	0.0
13	0.026373336556741133	0.0	0.0	0.0	0.0
14	0.09544636087201554	0.0	0.0	0.0	0.0
15	0.1604377973868419	0.0	0.0	0.0	0.0
16	0.19172997052361015	0.0	0.0	0.0	0.0
17	0.2129751583054294	0.0	0.0	0.0	0.0
18	0.23558087535406466	0.0	0.0	0.0	0.0
19	0.2606460106927135	0.0	0.0	0.0	0.0
20	0.2880135801752048	0.0	0.0	0.0	0.0
21	0.3172126313630253	0.0	0.0	0.0	0.0
22	0.35070258254618863	0.0	0.0	0.0	0.0
23	0.38393089348573356	0.0	0.0	0.0	0.0
24	0.42506073978255604	0.0	0.0	0.0	0.0
25	0.4684406921744973	0.0	0.0	0.0	0.0
26	0.5185186348030713	0.0	0.0	0.0	0.0
27	0.5661371591416317	0.0	0.0	0.0	0.0
28	0.6104590164105994	0.0	0.0	0.0	0.0
29	0.6623161126957788	0.0	0.0	0.0	0.0
30	0.7207665431201437	0.0	0.0	0.0	0.0
31	0.7745074491593761	0.0	0.0	0.0	0.0
32	0.8173117930153567	0.0	0.0	0.0	0.0
33	0.8580230149223896	0.0	0.0	0.0	0.0
34	0.8983679404883568	0.0	0.0	0.0	0.0
35	0.9331137648408887	0.0	0.0	0.0	0.0
36	0.9636733452955253	0.0	0.0	0.0	0.0
37	0.9970063123325176	0.0	0.0	0.0	0.0
38	1.0300776391258915	0.0	0.0	0.0	0.0
39	1.0679631464018449	0.0	0.0	0.0	0.0
40	1.1068952146522724	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATAAG	75	0.0	46.000004	33
ACAACGG	50	1.6370905E-11	46.0	38
AACGTTA	20	6.31251E-4	46.0	36
GATACCT	65	0.0	46.0	16
TCGTTAG	25	3.418012E-5	46.0	33
TCGCAAT	50	1.6370905E-11	46.0	26
CTCGTTA	25	3.418012E-5	46.0	32
GTATTCG	50	1.6370905E-11	46.0	30
CGAAATA	20	6.31251E-4	46.0	22
CGACGTA	20	6.31251E-4	46.0	36
ACGTTAT	30	1.8620558E-6	46.0	29
CCGATAT	55	1.8189894E-12	46.0	32
ATAATTC	20	6.31251E-4	46.0	13
GACGTTA	20	6.31251E-4	46.0	14
CTATACC	20	6.31251E-4	46.0	21
CCAATTG	25	3.418012E-5	46.0	29
ACCGATA	50	1.6370905E-11	46.0	31
TATATCG	20	6.31251E-4	46.0	17
CTAAGTA	30	1.8620558E-6	46.0	32
TACGGTA	25	3.418012E-5	46.0	32
>>END_MODULE