FastQCFastQC Report
Fri 17 Jun 2016
SRR1528045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885040
Sequences flagged as poor quality0
Sequence length59
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCTGGATGGGGGTGAATGGCCACCGGGGCCACCGTTATGCAATGTTCAGCGTGTCAC31150.1652484827908161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA457.6397555E-1153.00000436
ATAGCGA750.053.00000429
TAATTCG1500.053.00000443
TTACGCC457.6397555E-1153.00000417
AACGTTA307.0128954E-753.036
ACTATTA203.1387602E-453.039
CGGCGTA203.1387602E-453.033
GATACCT800.053.016
GTATTCG251.479097E-553.030
CAACGAA150.006696928353.048
CATGCGG150.006696928353.026
TCCGATT251.479097E-553.050
CTATCGA203.1387602E-453.028
ACCGATA203.1387602E-453.031
TTCACGC150.006696928353.052
TACGGTT203.1387602E-453.025
CGCGATT353.3436663E-853.022
TACGGTA150.006696928353.032
CGATTAG1000.053.037
GAATACG150.006696928353.048