##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528045_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1885040
Sequences flagged as poor quality	0
Sequence length	59
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21917678139456	31.0	31.0	33.0	30.0	34.0
2	31.804510779612105	31.0	31.0	34.0	30.0	34.0
3	32.09004795654204	33.0	31.0	34.0	30.0	34.0
4	35.84382983915461	37.0	35.0	37.0	35.0	37.0
5	35.427109769553965	37.0	35.0	37.0	33.0	37.0
6	35.640792768323216	37.0	35.0	37.0	33.0	37.0
7	35.649377201544795	37.0	35.0	37.0	33.0	37.0
8	34.8906893222425	36.0	35.0	37.0	32.0	37.0
9	37.47524986207189	39.0	37.0	39.0	35.0	39.0
10	37.27284938250647	39.0	37.0	39.0	33.0	39.0
11	37.9081069897721	39.0	37.0	39.0	35.0	39.0
12	37.990838921190004	39.0	38.0	39.0	35.0	39.0
13	37.80394421338539	39.0	38.0	39.0	35.0	39.0
14	37.76865902049824	40.0	38.0	40.0	33.0	40.0
15	35.96447237193906	38.0	35.0	40.0	31.0	40.0
16	35.59976870517337	38.0	35.0	40.0	27.0	40.0
17	35.464898888087255	38.0	34.0	40.0	27.0	40.0
18	35.65420892925349	38.0	34.0	40.0	29.0	40.0
19	36.186705321902984	38.0	35.0	40.0	30.0	40.0
20	36.370097186266605	38.0	35.0	40.0	31.0	40.0
21	36.400279569664306	38.0	35.0	40.0	31.0	40.0
22	36.380896426601026	38.0	35.0	40.0	30.0	40.0
23	36.272649386750416	38.0	35.0	40.0	30.0	40.0
24	36.25543967236769	38.0	35.0	40.0	30.0	40.0
25	36.14719475448797	38.0	35.0	40.0	30.0	40.0
26	36.040310019946524	38.0	35.0	40.0	30.0	40.0
27	35.86110002970759	38.0	35.0	40.0	30.0	40.0
28	35.815140792768325	38.0	35.0	40.0	30.0	40.0
29	35.754200971862666	38.0	35.0	40.0	30.0	40.0
30	35.679673110384925	38.0	34.0	40.0	29.0	40.0
31	35.64282243347621	38.0	34.0	40.0	29.0	40.0
32	35.43827929380809	38.0	34.0	40.0	28.0	40.0
33	35.251207931927176	38.0	34.0	40.0	27.0	40.0
34	35.19769766158808	38.0	34.0	40.0	27.0	40.0
35	35.05283336162628	37.0	34.0	40.0	27.0	40.0
36	34.85036975342698	37.0	33.0	40.0	26.0	40.0
37	34.68997952298094	37.0	33.0	40.0	25.0	40.0
38	34.462659147816495	37.0	33.0	40.0	24.0	40.0
39	34.28562683019989	36.0	33.0	40.0	24.0	40.0
40	34.005112358358446	36.0	33.0	40.0	23.0	40.0
41	33.818939651147986	35.0	33.0	40.0	23.0	40.0
42	33.61953486398166	35.0	33.0	40.0	23.0	40.0
43	33.35167264355133	35.0	32.0	39.0	22.0	40.0
44	33.1720361371642	35.0	32.0	39.0	21.0	40.0
45	32.96799219114714	35.0	32.0	39.0	21.0	40.0
46	32.764203412129184	35.0	31.0	39.0	20.0	40.0
47	32.643341255357974	35.0	31.0	39.0	20.0	40.0
48	32.45716165174214	35.0	31.0	38.0	20.0	40.0
49	32.337366846326866	35.0	31.0	38.0	20.0	40.0
50	32.08324544837245	35.0	31.0	38.0	19.0	40.0
51	31.90917434112804	35.0	31.0	37.0	18.0	40.0
52	31.6931874124687	35.0	30.0	37.0	18.0	40.0
53	31.432723974027077	35.0	30.0	37.0	18.0	40.0
54	31.223743262742435	34.0	30.0	36.0	17.0	40.0
55	31.095865870220262	34.0	30.0	36.0	17.0	39.0
56	30.85586831048678	34.0	29.0	36.0	15.0	39.0
57	30.65650914569452	34.0	29.0	35.0	14.0	39.0
58	30.49083573823367	34.0	29.0	35.0	13.0	39.0
59	29.880588740822475	33.0	27.0	35.0	10.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	5.0
14	17.0
15	107.0
16	324.0
17	881.0
18	1987.0
19	3447.0
20	6095.0
21	9152.0
22	12952.0
23	17344.0
24	22942.0
25	28949.0
26	36192.0
27	43757.0
28	49396.0
29	54528.0
30	62875.0
31	75984.0
32	93940.0
33	121296.0
34	183200.0
35	204499.0
36	207753.0
37	273211.0
38	300200.0
39	74003.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.391864363620932	20.45314688282477	34.60642744981539	16.54856130373891
2	21.456414717990068	22.79628018503586	35.014588549845094	20.732716547128973
3	19.816661715401267	23.85127106056105	35.25161269787379	21.0804545261639
4	19.641811314348768	22.494164580061963	36.96139073971905	20.902633365870223
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	99.03174468446294	0.6387132368543904	0.24439799685948307	0.0851440818231974
12	98.58045452616389	1.0185460255485295	0.13113780078937318	0.2698616474981963
13	93.29616347663709	3.413985910113313	1.7781055043924798	1.5117451088571063
14	54.38415099944829	27.298306667232524	7.52875270551288	10.788789627806308
15	32.37899461019395	30.523384119169883	17.084412001867335	20.013209268768833
16	28.42072316767814	21.697205364342402	25.87133429529347	24.010737172685992
17	21.98377753257225	21.560444340703647	28.09558417858507	28.360193948139035
18	23.230594576242414	22.033113355684762	29.395185248058397	25.34110682001443
19	25.69881806221619	21.59015193311548	27.21279124050418	25.498238764164157
20	27.43161948818062	20.920564019861647	24.646691847387853	27.001124644569874
21	30.993665916903616	22.124623350167635	19.81209947799516	27.069611254933584
22	29.42728005771761	22.38037389127021	21.864469719475448	26.327876331536732
23	28.686446971947543	23.63965751389891	20.620305139413485	27.05359037474006
24	29.56388193353987	24.318529049781436	21.311431057165894	24.806157959512795
25	31.668664855918177	22.27586682510716	21.217109451258327	24.838358867716334
26	30.39224631838051	22.95197979883716	20.447948054152697	26.207825828629634
27	29.66021941178967	24.748599499214873	19.83125026524636	25.7599308237491
28	30.022015447948053	23.716791155625344	20.744281288460723	25.51691210796588
29	30.857594533802995	22.602119848915674	20.024827059372747	26.515458557908584
30	30.03543691380554	22.521644103042906	20.161004541017697	27.281914442133854
31	30.181799855705982	22.988530747358148	19.418951321987862	27.410718074948008
32	30.011564741331746	24.87957815218775	19.938568942834102	25.170288163646394
33	30.625981411535037	24.982334592369394	19.987002928319825	24.404681067775748
34	27.804290625132623	26.459279378686922	19.66382676229682	26.07260323388363
35	30.094056359546745	26.099499214870768	19.726477952722487	24.079966472859994
36	31.68054789288291	24.710669269617622	20.09702711878793	23.511755718711537
37	30.236546704579215	25.815685608793448	19.529877350082756	24.41789033654458
38	29.769607010991813	26.822295548105078	21.28060943003862	22.12748801086449
39	30.830645503543693	26.56818953443959	20.59049144845733	22.010673513559396
40	30.64147179900692	26.708133514408182	20.14684038534991	22.50355430123499
41	31.77025421211221	26.065759877774475	19.99230785553622	22.17167805457709
42	32.52567584772737	26.410845393201203	18.139827271569832	22.92365148750159
43	30.955682638034205	26.084910665025674	19.129673640877648	23.829733056062473
44	29.499374018588465	26.564900479565424	21.064274498153885	22.87145100369223
45	28.54623774561813	24.21248355472563	20.933030598820185	26.308248100836057
46	28.968563001315623	23.060253363323856	21.450791495140685	26.520392140219833
47	28.290805500148537	23.442897763442687	22.75564444255825	25.510652293850526
48	27.711825743750794	24.281341509994483	21.99184102194118	26.01499172431354
49	28.531383949412216	23.197438781139923	22.655911810889954	25.615265458557907
50	27.721268514195984	24.245002758562155	21.049049357042822	26.98467937019904
51	28.365658023171925	25.181640707889485	21.942027755379197	24.510673513559393
52	29.264259644357683	24.869127445571447	22.067807579680007	23.798805330390866
53	27.84858676738955	24.886527606841234	22.168601196791577	25.096284428977633
54	26.78516105759029	25.559351525697068	21.39933370114162	26.256153715571024
55	27.87786996562407	25.524020710435853	21.367344990026737	25.230764333913342
56	27.89664940797012	22.73781988711115	22.282285787039	27.08324491787973
57	26.93014471841446	23.60708526079022	22.424245639349827	27.038524381445487
58	28.380299622289186	23.72750710860247	20.87292577345839	27.019267495649963
59	27.577504986631585	23.615732292152952	21.913911641132284	26.89285108008318
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	1.0
7	1.0
8	38.5
9	256.5
10	437.0
11	681.5
12	926.0
13	1156.0
14	1253.0
15	1120.0
16	1093.0
17	1066.0
18	1142.0
19	1548.0
20	1878.0
21	2305.0
22	2732.0
23	3673.5
24	4615.0
25	5937.0
26	9162.0
27	11065.0
28	13326.0
29	15587.0
30	17762.0
31	21326.0
32	22715.0
33	24163.5
34	25612.0
35	25778.0
36	27747.5
37	29551.0
38	32137.0
39	34723.0
40	34952.0
41	35181.0
42	35774.5
43	42472.5
44	48577.0
45	50081.0
46	51585.0
47	51203.0
48	56405.5
49	61990.0
50	66064.5
51	70139.0
52	78281.0
53	95928.5
54	105434.0
55	115813.0
56	126192.0
57	131530.0
58	147177.0
59	157486.0
60	151746.5
61	146007.0
62	139633.0
63	133259.0
64	125795.0
65	103538.5
66	88746.0
67	78630.0
68	68514.0
69	60890.0
70	44378.5
71	35491.0
72	32816.0
73	30141.0
74	25999.0
75	14517.0
76	7177.0
77	5445.0
78	3713.0
79	2785.0
80	1857.0
81	1244.0
82	494.5
83	358.0
84	419.0
85	480.0
86	308.5
87	128.0
88	119.0
89	79.5
90	40.0
91	21.5
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1885040.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.46501052972896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.78372262764834	15.655323310691463
2	5.68882706127041	2.100885031763304
3	2.5084339576744172	1.389547783247634
4	1.3311001249401562	0.9831511129257408
5	0.8568426187053217	0.7910803988357152
6	0.5740331188494716	0.6359716550381189
7	0.37789607033645434	0.4884498442534078
8	0.2888755641248096	0.4267272266676802
9	0.2235878941263967	0.37156975374274553
>10	1.4159854303653923	5.286709275798394
>50	0.4464479232213985	6.1364909256158136
>100	1.4312760860829177	56.57667769169622
>500	0.06722573346915764	7.805327828993404
>1k	0.005745789185398089	1.352088160730366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCCTGGATGGGGGTGAATGGCCACCGGGGCCACCGTTATGCAATGTTCAGCGTGTCAC	3115	0.1652484827908161	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.003660399779315028	0.0	0.0	0.0	0.0
12	0.011246445698765014	0.0	0.0	0.0	0.0
13	0.02604719263251708	0.0	0.0	0.0	0.0
14	0.08578067308916522	0.0	0.0	0.0	0.0
15	0.14249034503246616	0.0	0.0	0.0	0.0
16	0.1720918388999703	0.0	0.0	0.0	0.0
17	0.19156092178415313	0.0	0.0	0.0	0.0
18	0.21415991172601112	0.0	0.0	0.0	0.0
19	0.23829733056062471	0.0	0.0	0.0	0.0
20	0.26476891737045366	0.0	0.0	0.0	0.0
21	0.2909752578194627	0.0	0.0	0.0	0.0
22	0.32370665874464205	0.0	0.0	0.0	0.0
23	0.3548996307770657	0.0	0.0	0.0	0.0
24	0.3913444807537241	0.0	0.0	0.0	0.0
25	0.429274710350974	0.0	0.0	0.0	0.0
26	0.47203242371514664	0.0	0.0	0.0	0.0
27	0.5131456096422357	0.0	0.0	0.0	0.0
28	0.5551075839239485	0.0	0.0	0.0	0.0
29	0.6025336332385519	0.0	0.0	0.0	0.0
30	0.6528243432500106	0.0	0.0	0.0	0.0
31	0.7003564911089419	0.0	0.0	0.0	0.0
32	0.7377562279845521	0.0	0.0	0.0	0.0
33	0.7709650723592072	0.0	0.0	0.0	0.0
34	0.8080465136018334	0.0	0.0	0.0	0.0
35	0.8381254509188134	0.0	0.0	0.0	0.0
36	0.865923269532742	0.0	0.0	0.0	0.0
37	0.8965857488435258	0.0	0.0	0.0	0.0
38	0.9282561643254255	0.0	0.0	0.0	0.0
39	0.9617302550609006	0.0	0.0	0.0	0.0
40	0.9982281543097229	0.0	0.0	0.0	0.0
41	1.0371132708059245	0.0	0.0	0.0	0.0
42	1.0778551118278656	0.0	0.0	0.0	0.0
43	1.118331706488987	0.0	0.0	0.0	0.0
44	1.1605058778593558	0.0	0.0	0.0	0.0
45	1.1995501421720494	0.0	0.0	0.0	0.0
46	1.2353584008827398	0.0	0.0	0.0	0.0
47	1.2696282307006748	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTA	45	7.6397555E-11	53.000004	36
ATAGCGA	75	0.0	53.000004	29
TAATTCG	150	0.0	53.000004	43
TTACGCC	45	7.6397555E-11	53.000004	17
AACGTTA	30	7.0128954E-7	53.0	36
ACTATTA	20	3.1387602E-4	53.0	39
CGGCGTA	20	3.1387602E-4	53.0	33
GATACCT	80	0.0	53.0	16
GTATTCG	25	1.479097E-5	53.0	30
CAACGAA	15	0.0066969283	53.0	48
CATGCGG	15	0.0066969283	53.0	26
TCCGATT	25	1.479097E-5	53.0	50
CTATCGA	20	3.1387602E-4	53.0	28
ACCGATA	20	3.1387602E-4	53.0	31
TTCACGC	15	0.0066969283	53.0	52
TACGGTT	20	3.1387602E-4	53.0	25
CGCGATT	35	3.3436663E-8	53.0	22
TACGGTA	15	0.0066969283	53.0	32
CGATTAG	100	0.0	53.0	37
GAATACG	15	0.0066969283	53.0	48
>>END_MODULE