FastQCFastQC Report
Fri 17 Jun 2016
SRR1528044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4042782
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTTCATAGGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCC48890.12093157632541156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTA206.3133484E-446.00000430
ACGTTAG206.3133484E-446.00000442
ACCGATT206.3133484E-446.00000428
TCGATAG206.3133484E-446.00000430
CGGATTA206.3133484E-446.00000415
GCGATAA206.3133484E-446.00000431
ATACGGT206.3133484E-446.00000419
TATACGC206.3133484E-446.00000436
TACGTTG405.6170393E-946.00000421
TACGTTA206.3133484E-446.00000428
CTTAACC501.6370905E-1146.035
ACGTTAC453.110472E-1046.028
CTATACG301.862576E-646.035
GCACGAC253.4186927E-546.028
CGATTAT253.4186927E-546.040
TACGGGT551.8189894E-1246.018
CTACGGG551.8189894E-1246.017
AACGATA501.6370905E-1146.018
CGAATAC301.862576E-646.030
ACTCGAT501.6370905E-1146.025
Produced by FastQC (version 0.11.5)