##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528044_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4042782
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.831739381445747	31.0	31.0	34.0	30.0	34.0
2	32.34483605596344	34.0	31.0	34.0	30.0	34.0
3	32.44991567687795	34.0	31.0	34.0	30.0	34.0
4	35.952283353393774	37.0	35.0	37.0	35.0	37.0
5	35.84456495551825	37.0	35.0	37.0	35.0	37.0
6	36.0848603758501	37.0	35.0	37.0	35.0	37.0
7	36.07205780573872	37.0	36.0	37.0	35.0	37.0
8	36.143545954246356	37.0	36.0	37.0	35.0	37.0
9	38.08757113294756	39.0	39.0	39.0	35.0	39.0
10	37.78432846490362	39.0	38.0	39.0	35.0	39.0
11	38.01341205140421	39.0	37.0	39.0	35.0	39.0
12	38.001978340657494	39.0	38.0	39.0	35.0	39.0
13	38.044675671357	39.0	38.0	39.0	37.0	39.0
14	38.75637469445545	40.0	39.0	40.0	36.0	40.0
15	38.228777361727644	40.0	38.0	40.0	34.0	40.0
16	37.60068314343935	40.0	36.0	40.0	33.0	40.0
17	37.52600437025791	40.0	37.0	40.0	33.0	40.0
18	37.5368847491653	40.0	37.0	40.0	33.0	40.0
19	37.67854264711775	40.0	37.0	40.0	33.0	40.0
20	37.712475715979735	40.0	37.0	40.0	33.0	40.0
21	37.48792786749323	40.0	37.0	40.0	33.0	40.0
22	37.58201208969467	40.0	37.0	40.0	33.0	40.0
23	37.50761579526178	40.0	36.0	40.0	33.0	40.0
24	37.487403723475566	40.0	36.0	40.0	33.0	40.0
25	37.593311734345306	40.0	37.0	40.0	33.0	40.0
26	37.584038911818645	40.0	37.0	40.0	33.0	40.0
27	37.56570871246582	40.0	36.0	40.0	33.0	40.0
28	37.536698738640865	40.0	36.0	40.0	33.0	40.0
29	37.55816539204934	40.0	36.0	40.0	33.0	40.0
30	37.523684927854134	40.0	36.0	40.0	33.0	40.0
31	37.49309609076126	40.0	36.0	40.0	33.0	40.0
32	37.44123502083466	40.0	36.0	40.0	33.0	40.0
33	37.357781102221196	40.0	35.0	40.0	33.0	40.0
34	37.253584289234496	40.0	35.0	40.0	33.0	40.0
35	37.16190410464873	40.0	35.0	40.0	33.0	40.0
36	37.09019531599775	39.0	35.0	40.0	33.0	40.0
37	36.946640951701085	39.0	35.0	40.0	32.0	40.0
38	36.73970820093688	39.0	35.0	40.0	32.0	40.0
39	36.61771275324764	39.0	35.0	40.0	31.0	40.0
40	36.49360316732389	39.0	35.0	40.0	31.0	40.0
41	36.34446527168667	38.0	35.0	40.0	31.0	40.0
42	36.19046389342784	38.0	35.0	40.0	31.0	40.0
43	36.012131497567765	38.0	35.0	40.0	31.0	40.0
44	35.84301255917336	38.0	35.0	40.0	30.0	40.0
45	35.68419568505054	37.0	35.0	40.0	30.0	40.0
46	35.530733539429036	37.0	35.0	40.0	30.0	40.0
47	35.38257022020974	37.0	35.0	40.0	30.0	40.0
48	35.227099061982564	36.0	34.0	40.0	29.0	40.0
49	35.057314987550654	36.0	34.0	40.0	29.0	40.0
50	34.902176768373856	36.0	34.0	40.0	29.0	40.0
51	34.72489092906815	35.0	34.0	39.0	28.0	40.0
52	34.10375281180138	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	7.0
14	7.0
15	13.0
16	70.0
17	184.0
18	429.0
19	924.0
20	1832.0
21	3177.0
22	5233.0
23	7934.0
24	11925.0
25	16703.0
26	23244.0
27	31626.0
28	41978.0
29	55124.0
30	70811.0
31	88073.0
32	109103.0
33	139966.0
34	212240.0
35	404994.0
36	343103.0
37	673882.0
38	1233446.0
39	566751.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.16863140283102	18.607632071182667	35.18799678043486	16.035739745551457
2	24.441115053940578	22.184203847746428	36.175138802933226	17.199542295379764
3	22.047342646721983	22.25920170813069	36.41420685062909	19.27924879451823
4	22.61623307910246	22.437568980964098	35.797750163130246	19.148447776803202
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.6147702250579	0.30753575137120925	0.04667577920352866	0.031018244367368806
12	99.4182718731804	0.4955498466155237	0.05721307752928553	0.028965202674791763
13	98.48413790305784	0.9222114870403598	0.2529693661444025	0.3406812437573928
14	67.02273335539735	21.788090478289455	3.7843989609135495	7.404777205399649
15	36.350587293601286	23.805785224135263	17.06827130426523	22.775356177998223
16	30.96501369601428	15.333995253763375	28.44815772900938	25.252833321212965
17	23.262693857843438	15.503136206701226	30.19309475504739	31.041075180407944
18	23.60241041935974	17.477642870676675	32.54506426515207	26.37488244481152
19	25.70959799464824	16.694098271932546	30.055639903413045	27.540663830006167
20	29.01722130948441	17.17265486983963	25.908421478081184	27.901702342594774
21	33.086498356824585	17.30372797741753	21.81638782402811	27.79338584172978
22	31.944735085888876	18.62210230479902	21.881293624044037	27.55186898526806
23	29.816596591159257	20.102370100589148	21.00805831232058	29.072974995931023
24	30.140630882397318	21.336421305922507	21.25207344843229	27.27087436324788
25	31.785488309782718	19.97001075002313	21.685685748081397	26.558815192112757
26	30.60595401879201	19.37739902868866	22.129241695446353	27.887405257072977
27	31.21991242664086	21.122756557242017	20.717194248910776	26.940136767206347
28	31.52185796810216	19.842524281546716	19.21461508436517	29.421002665985945
29	30.33079696110253	20.238761328214085	19.678206739814318	29.75223497086907
30	29.92026282891336	18.813406213839876	20.221842285831883	31.044488671414882
31	28.399156818250404	18.121358015346857	21.0027896631577	32.47669550324504
32	30.861545341796813	21.415945752207264	19.12702688396258	28.59548202203334
33	32.85136324441931	20.64758871489979	19.46879648717146	27.03225155350944
34	29.65287270003676	20.59601531816457	20.825238659912902	28.92587332188577
35	32.07175158096578	21.89390869950445	19.18641668039484	26.84792303913493
36	34.95657198434148	20.65676556391119	19.658368915266763	24.72829353648057
37	34.152966942071075	20.500041802897115	20.572764002610082	24.774227252421724
38	31.807923355748592	22.742903277990255	22.138220660921117	23.310952705340036
39	31.273390452416184	24.686490639366653	22.169214169846406	21.870904738370754
40	31.72087933507174	24.155544375135733	19.866641337573977	24.256934952218547
41	31.56675279547599	24.762997361717748	18.915217293438033	24.755032549368234
42	32.93796202713874	23.923278574011658	18.825576051342864	24.313183347506744
43	32.499377903631704	22.718860428289233	18.062413456872026	26.719348211207034
44	30.559352445914715	24.03218872548656	18.69875743980259	26.70970138879613
45	29.237218331337182	23.604809757241423	20.537887029278355	26.62008488214304
46	28.990803857343778	21.9431817001263	20.890045518160512	28.175968924369403
47	29.70924477253535	19.667248938973213	22.797791223964094	27.825715064527344
48	29.500428170502392	18.66229739817779	21.895986476639106	29.94128795468071
49	30.285630043865837	19.844923619428403	21.96633407391247	27.903112262793293
50	29.94603715955993	21.466925498332586	20.24447521533439	28.342562126773096
51	28.079005991418782	21.247324243553077	22.652866268821818	28.020803496206327
52	30.193193696815708	20.703812374745905	22.27809958587923	26.82489434255916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	4.0
4	8.0
5	8.0
6	8.0
7	86.5
8	165.0
9	274.0
10	383.0
11	561.0
12	739.0
13	1097.5
14	1694.0
15	1932.0
16	2544.5
17	3157.0
18	3940.5
19	4724.0
20	6940.0
21	9156.0
22	12053.0
23	14950.0
24	18567.0
25	22184.0
26	22694.0
27	23204.0
28	23621.0
29	24038.0
30	24966.0
31	25894.0
32	27613.5
33	29333.0
34	32597.5
35	35862.0
36	40410.0
37	44958.0
38	52430.5
39	67661.5
40	75420.0
41	87791.0
42	100162.0
43	117289.5
44	134417.0
45	142865.5
46	151314.0
47	171120.0
48	190926.0
49	210266.5
50	229607.0
51	260678.5
52	291750.0
53	316211.5
54	340673.0
55	374060.5
56	407448.0
57	405726.0
58	404004.0
59	378096.0
60	352188.0
61	320919.5
62	289651.0
63	254047.5
64	200559.0
65	182674.0
66	162672.5
67	142671.0
68	121221.5
69	99772.0
70	78385.5
71	56999.0
72	49331.0
73	41663.0
74	29339.5
75	17016.0
76	12826.5
77	8637.0
78	5568.5
79	2500.0
80	2238.0
81	1976.0
82	1245.5
83	515.0
84	391.5
85	268.0
86	148.0
87	28.0
88	16.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	4042782.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.690918442583279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.66304264524648	5.665364532171247
2	8.21582373652125	1.263744605924495
3	3.887258180997107	0.8968975690594014
4	2.338290892654618	0.7193441820176768
5	1.4904136871372873	0.5731325056741354
6	0.9932048209025232	0.45831943646051027
7	0.6772404036889208	0.36460204975555666
8	0.49000142104372163	0.30148487727977386
9	0.3636178232297645	0.2516899520456804
>10	2.6452997780182104	4.434720422513335
>50	1.144342062572217	6.596547140534716
>100	3.8214842146534873	64.37385046420235
>500	0.25282330975332085	12.391656808827035
>1k	0.017157023581211274	1.7086454535340967
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCGTTCATAGGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCC	4889	0.12093157632541156	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.02028306250497801	0.0	0.0	0.0	0.0
11	0.02149509916686084	0.0	0.0	0.0	0.0
12	0.028544700159444658	0.0	0.0	0.0	0.0
13	0.034011232858957026	0.0	0.0	0.0	0.0
14	0.04976770946343384	0.0	0.0	0.0	0.0
15	0.07094124788326454	0.0	0.0	0.0	0.0
16	0.08293793729169666	0.0	0.0	0.0	0.0
17	0.09194163820854055	0.0	0.0	0.0	0.0
18	0.10492774530014233	0.0	0.0	0.0	0.0
19	0.11848276755956666	0.0	0.0	0.0	0.0
20	0.13146887465116844	0.0	0.0	0.0	0.0
21	0.14598857915168317	0.0	0.0	0.0	0.0
22	0.1656779910467594	0.0	0.0	0.0	0.0
23	0.18403168906955655	0.0	0.0	0.0	0.0
24	0.19963975302155793	0.0	0.0	0.0	0.0
25	0.2196012547795058	0.0	0.0	0.0	0.0
26	0.24589502970973948	0.0	0.0	0.0	0.0
27	0.2701110275053169	0.0	0.0	0.0	0.0
28	0.2913340368093061	0.0	0.0	0.0	0.0
29	0.3190377319380565	0.0	0.0	0.0	0.0
30	0.35854023293860515	0.0	0.0	0.0	0.0
31	0.39146310634607556	0.0	0.0	0.0	0.0
32	0.41545648516293976	0.0	0.0	0.0	0.0
33	0.4356158704575216	0.0	0.0	0.0	0.0
34	0.4559731392887373	0.0	0.0	0.0	0.0
35	0.47482154615311933	0.0	0.0	0.0	0.0
36	0.49305156696552027	0.0	0.0	0.0	0.0
37	0.5106632017259402	0.0	0.0	0.0	0.0
38	0.5319109464720086	0.0	0.0	0.0	0.0
39	0.5559537961730313	0.0	0.0	0.0	0.0
40	0.5856115912260419	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATTA	20	6.3133484E-4	46.000004	30
ACGTTAG	20	6.3133484E-4	46.000004	42
ACCGATT	20	6.3133484E-4	46.000004	28
TCGATAG	20	6.3133484E-4	46.000004	30
CGGATTA	20	6.3133484E-4	46.000004	15
GCGATAA	20	6.3133484E-4	46.000004	31
ATACGGT	20	6.3133484E-4	46.000004	19
TATACGC	20	6.3133484E-4	46.000004	36
TACGTTG	40	5.6170393E-9	46.000004	21
TACGTTA	20	6.3133484E-4	46.000004	28
CTTAACC	50	1.6370905E-11	46.0	35
ACGTTAC	45	3.110472E-10	46.0	28
CTATACG	30	1.862576E-6	46.0	35
GCACGAC	25	3.4186927E-5	46.0	28
CGATTAT	25	3.4186927E-5	46.0	40
TACGGGT	55	1.8189894E-12	46.0	18
CTACGGG	55	1.8189894E-12	46.0	17
AACGATA	50	1.6370905E-11	46.0	18
CGAATAC	30	1.862576E-6	46.0	30
ACTCGAT	50	1.6370905E-11	46.0	25
>>END_MODULE