FastQCFastQC Report
Fri 17 Jun 2016
SRR1528043_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528043_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4008999
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTTCATAGGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCC46250.11536545656409493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC850.046.00000428
TATGCGC351.0204167E-746.00000431
CGTTACA850.046.00000429
ACGTATC551.8189894E-1246.00000429
TCGTTAG301.8625706E-646.043
CGAACTT206.313344E-446.020
CTATACG453.110472E-1046.035
CGATTAT206.313344E-446.040
AACGGAC453.110472E-1046.021
CTACGTA501.6370905E-1146.027
TACGATA206.313344E-446.039
AACGATA206.313344E-446.018
CGAATAT206.313344E-446.046
ACGTCTA501.6370905E-1146.023
CATAACG453.110472E-1046.022
CGTAAGT405.6170393E-946.014
CGGTATA253.418688E-546.042
TGTACGT206.313344E-446.045
TACGTTG253.418688E-546.021
GTACGTA206.313344E-446.032
Produced by FastQC (version 0.11.5)