##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528043_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4008999
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.776062802709603	31.0	31.0	34.0	30.0	34.0
2	32.18181321571794	34.0	31.0	34.0	30.0	34.0
3	32.38013229736401	34.0	31.0	34.0	30.0	34.0
4	35.92302243028746	37.0	35.0	37.0	35.0	37.0
5	35.82233794520777	37.0	35.0	37.0	35.0	37.0
6	36.06688652204703	37.0	35.0	37.0	35.0	37.0
7	36.066756314980374	37.0	36.0	37.0	35.0	37.0
8	36.09122626371321	37.0	36.0	37.0	35.0	37.0
9	38.07446646906123	39.0	39.0	39.0	35.0	39.0
10	37.77063301836693	39.0	38.0	39.0	35.0	39.0
11	37.99842903428013	39.0	37.0	39.0	35.0	39.0
12	37.96629881923143	39.0	37.0	39.0	35.0	39.0
13	38.04999776752252	39.0	39.0	39.0	37.0	39.0
14	38.783270586996906	40.0	39.0	40.0	36.0	40.0
15	38.2511781619302	40.0	38.0	40.0	35.0	40.0
16	37.62858983002989	40.0	36.0	40.0	33.0	40.0
17	37.565702061786496	40.0	37.0	40.0	33.0	40.0
18	37.59723112926693	40.0	37.0	40.0	33.0	40.0
19	37.73067965344965	40.0	37.0	40.0	33.0	40.0
20	37.743029369675575	40.0	37.0	40.0	33.0	40.0
21	37.70862003208282	40.0	37.0	40.0	33.0	40.0
22	37.69614409981145	40.0	37.0	40.0	33.0	40.0
23	37.60126355731194	40.0	37.0	40.0	33.0	40.0
24	37.58098293364503	40.0	37.0	40.0	33.0	40.0
25	37.546911336221335	40.0	36.0	40.0	33.0	40.0
26	37.516343605972466	40.0	36.0	40.0	33.0	40.0
27	37.530803824096736	40.0	36.0	40.0	33.0	40.0
28	37.35387013067352	40.0	36.0	40.0	33.0	40.0
29	37.47838575165521	40.0	36.0	40.0	33.0	40.0
30	37.50783549709042	40.0	36.0	40.0	33.0	40.0
31	37.51818646001159	40.0	36.0	40.0	33.0	40.0
32	37.4577708799628	40.0	36.0	40.0	33.0	40.0
33	37.37337574791114	40.0	36.0	40.0	33.0	40.0
34	37.29217991822897	40.0	35.0	40.0	33.0	40.0
35	37.128542561372555	40.0	35.0	40.0	33.0	40.0
36	36.682583108651315	39.0	35.0	40.0	31.0	40.0
37	36.756771702861485	39.0	35.0	40.0	31.0	40.0
38	36.66866716604319	39.0	35.0	40.0	31.0	40.0
39	36.579122868326984	39.0	35.0	40.0	31.0	40.0
40	36.46997342728197	39.0	35.0	40.0	31.0	40.0
41	36.356446584296975	38.0	35.0	40.0	31.0	40.0
42	36.05721403272987	38.0	35.0	40.0	31.0	40.0
43	35.87667021119237	38.0	35.0	40.0	30.0	40.0
44	35.71568114634102	38.0	35.0	40.0	30.0	40.0
45	35.31932534779879	37.0	35.0	40.0	29.0	40.0
46	35.32176885052852	37.0	35.0	40.0	29.0	40.0
47	35.25767878714861	37.0	34.0	40.0	29.0	40.0
48	35.09785609824298	36.0	34.0	40.0	29.0	40.0
49	34.93457643666162	36.0	34.0	40.0	28.0	40.0
50	34.7160191359489	36.0	34.0	40.0	28.0	40.0
51	34.6291036740094	35.0	34.0	39.0	28.0	40.0
52	34.14544827773716	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	4.0
14	6.0
15	21.0
16	59.0
17	166.0
18	454.0
19	919.0
20	1810.0
21	3195.0
22	5258.0
23	8318.0
24	12120.0
25	17536.0
26	23923.0
27	31970.0
28	43228.0
29	55747.0
30	71397.0
31	89167.0
32	111208.0
33	143235.0
34	217035.0
35	399644.0
36	347584.0
37	675630.0
38	1190168.0
39	559195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.18307063683478	18.568126357726705	35.21320409408932	16.03559891134919
2	24.574039554512236	22.24375710744752	35.987636814077526	17.194566523962713
3	22.075959609867702	22.222280424614723	36.4484750432714	19.253284922246177
4	22.600554402732453	22.445154014755303	35.76907851560951	19.185213066902733
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.6157893778472	0.3013470444866661	0.05036169876819625	0.03250187889794934
12	99.4140183123019	0.4971315782318729	0.06066352224083867	0.028186587225389678
13	98.47977013713398	0.92297354027776	0.2534049023210033	0.34385142026725374
14	66.98230655582603	21.821856278836687	3.801397805287554	7.394439360049729
15	36.34144084346242	23.809609331406666	17.10304741906895	22.745902406061962
16	30.98314566803334	15.335748400037016	28.430812778950553	25.250293152979086
17	23.249993327511433	15.476406953456461	30.13969322516668	31.133906493865425
18	23.59925757028126	17.446175466743693	32.588708552933035	26.36585841004201
19	25.688906382865152	16.714172290888573	30.039144434807792	27.557776891438486
20	29.080151928199534	17.176357489737462	25.847225205094837	27.896265376968167
21	32.84984605882915	17.406539637450646	21.784041352966163	27.95957295075404
22	31.987186826437224	18.649692853502835	21.8734901156124	27.489630204447547
23	29.766283304136522	20.121257201610675	21.022629339643135	29.08983015460967
24	30.180750855762252	21.34829666956764	21.22874064074349	27.24221183392662
25	31.838371623440164	19.92330255008794	21.646750223684265	26.591575602787625
26	30.600656173773054	19.39588909850065	22.13033727371845	27.873117454007847
27	31.25149195597205	21.098458742444187	20.707238889308776	26.942810412274987
28	31.621559396747166	19.865806900924646	19.220034726873216	29.29259897545497
29	30.380351803530008	20.24373166468737	19.677106429809534	29.698810101973088
30	29.92712145849874	18.827293296905285	20.24869549730494	30.996889747291036
31	28.414225097087826	18.098033948125206	20.994966573950254	32.492774380836714
32	30.767730298760366	21.454931767256614	19.14944353939724	28.62789439458578
33	32.86680789892939	20.60629099682988	19.494093163904505	27.03280794033623
34	29.641563891634792	20.613873937109986	20.807687904137666	28.936874267117553
35	32.13577753449178	21.91327560820045	19.17758023885763	26.77336661845014
36	35.03363308396934	20.579850481379516	19.723102949140173	24.663413485510972
37	34.070275397923524	20.48089809950065	20.604270542347354	24.844555960228476
38	31.840816123925197	22.712502547394	22.183866845564193	23.26281448311661
39	31.24702700100449	24.67558610017114	22.175236262219073	21.902150636605295
40	31.66079612392021	24.24453086668268	19.84008975806679	24.25458325133032
41	31.591776401041756	24.756329447824758	18.93233198611424	24.719562165019248
42	33.05161712437444	23.906092268918	18.82554722513026	24.216743381577295
43	32.475737709088975	22.73358012810679	18.061765542969706	26.728916619834525
44	30.85500894362907	23.95366025284616	18.56720343407419	26.62412736945058
45	29.245430093646817	23.650816575409472	20.589852978262154	26.513900352681553
46	29.03784710347895	22.010606637716794	20.864335461295948	28.087210797508305
47	29.709885185803238	19.687732523754683	22.71896301296159	27.88341927748049
48	29.49851072549532	18.677205955900714	21.944031415323376	29.880251903280598
49	30.35909961563971	19.839915150889286	21.913525047025452	27.887460186445544
50	30.0326340814752	21.45740121162415	20.296986853825604	28.212977853075046
51	28.11422502225618	21.154058656537455	22.62205602944775	28.109660291758615
52	30.21632083220774	20.684564900115966	22.299556572600792	26.799557695075503
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.5
4	5.0
5	7.5
6	10.0
7	84.5
8	159.0
9	258.5
10	358.0
11	540.0
12	722.0
13	1114.0
14	1743.0
15	1980.0
16	2572.5
17	3165.0
18	3944.0
19	4723.0
20	6772.5
21	8822.0
22	11758.0
23	14694.0
24	18074.0
25	21454.0
26	22343.5
27	23233.0
28	23661.0
29	24089.0
30	24913.5
31	25738.0
32	27535.5
33	29333.0
34	32104.5
35	34876.0
36	39555.5
37	44235.0
38	51792.0
39	67239.5
40	75130.0
41	87122.0
42	99114.0
43	115836.0
44	132558.0
45	141723.5
46	150889.0
47	170258.5
48	189628.0
49	208775.5
50	227923.0
51	258828.0
52	289733.0
53	314240.5
54	338748.0
55	371056.0
56	403364.0
57	401883.5
58	400403.0
59	375397.0
60	350391.0
61	318239.0
62	286087.0
63	251374.0
64	198525.5
65	180390.0
66	160714.5
67	141039.0
68	120087.0
69	99135.0
70	78232.5
71	57330.0
72	49258.0
73	41186.0
74	29122.0
75	17058.0
76	12847.0
77	8636.0
78	5539.5
79	2443.0
80	2176.5
81	1910.0
82	1218.5
83	527.0
84	379.5
85	232.0
86	130.0
87	28.0
88	16.5
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	4008999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.211845904136512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.48758057634721	6.034686875607789
2	8.604558732066696	1.4131862076164794
3	4.059317351271521	1.0000346569388796
4	2.360923068951552	0.7755014573500485
5	1.4687916719685994	0.6030745437742582
6	0.9848677786793393	0.4852549460678375
7	0.6546615965309218	0.37631861050475446
8	0.46006568916182256	0.30223908361418034
9	0.3452427589492087	0.25515723024088643
>10	2.529294308982241	4.58870258404027
>50	1.1539671789443442	7.080950527786549
>100	3.6663733656925888	64.69541820170276
>500	0.21236502927088358	11.085222972938093
>1k	0.011990893183199965	1.3042521018172093
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCGTTCATAGGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCC	4625	0.11536545656409493	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.019830386587774154	0.0	0.0	0.0	0.0
11	0.021102524595291744	0.0	0.0	0.0	0.0
12	0.02763782181038209	0.0	0.0	0.0	0.0
13	0.0327014299579521	0.0	0.0	0.0	0.0
14	0.04824146875566694	0.0	0.0	0.0	0.0
15	0.06772264098843626	0.0	0.0	0.0	0.0
16	0.07937143411609732	0.0	0.0	0.0	0.0
17	0.08979797700124145	0.0	0.0	0.0	0.0
18	0.10224497436891354	0.0	0.0	0.0	0.0
19	0.1166874823366132	0.0	0.0	0.0	0.0
20	0.129982571709297	0.0	0.0	0.0	0.0
21	0.14467451850200014	0.0	0.0	0.0	0.0
22	0.16492895109228015	0.0	0.0	0.0	0.0
23	0.18328764861253397	0.0	0.0	0.0	0.0
24	0.20207039213529362	0.0	0.0	0.0	0.0
25	0.2224744880205757	0.0	0.0	0.0	0.0
26	0.24726870722591848	0.0	0.0	0.0	0.0
27	0.27188831925375884	0.0	0.0	0.0	0.0
28	0.2934897214990575	0.0	0.0	0.0	0.0
29	0.3224994568469586	0.0	0.0	0.0	0.0
30	0.3613620257824958	0.0	0.0	0.0	0.0
31	0.39518593045296346	0.0	0.0	0.0	0.0
32	0.4183587972957838	0.0	0.0	0.0	0.0
33	0.4398853678935814	0.0	0.0	0.0	0.0
34	0.45909215741884696	0.0	0.0	0.0	0.0
35	0.4770766967015956	0.0	0.0	0.0	0.0
36	0.49388887350682803	0.0	0.0	0.0	0.0
37	0.5109754330195643	0.0	0.0	0.0	0.0
38	0.5318784065548532	0.0	0.0	0.0	0.0
39	0.5565479063476942	0.0	0.0	0.0	0.0
40	0.5852084273405905	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	85	0.0	46.000004	28
TATGCGC	35	1.0204167E-7	46.000004	31
CGTTACA	85	0.0	46.000004	29
ACGTATC	55	1.8189894E-12	46.000004	29
TCGTTAG	30	1.8625706E-6	46.0	43
CGAACTT	20	6.313344E-4	46.0	20
CTATACG	45	3.110472E-10	46.0	35
CGATTAT	20	6.313344E-4	46.0	40
AACGGAC	45	3.110472E-10	46.0	21
CTACGTA	50	1.6370905E-11	46.0	27
TACGATA	20	6.313344E-4	46.0	39
AACGATA	20	6.313344E-4	46.0	18
CGAATAT	20	6.313344E-4	46.0	46
ACGTCTA	50	1.6370905E-11	46.0	23
CATAACG	45	3.110472E-10	46.0	22
CGTAAGT	40	5.6170393E-9	46.0	14
CGGTATA	25	3.418688E-5	46.0	42
TGTACGT	20	6.313344E-4	46.0	45
TACGTTG	25	3.418688E-5	46.0	21
GTACGTA	20	6.313344E-4	46.0	32
>>END_MODULE