FastQCFastQC Report
Fri 17 Jun 2016
SRR1528042_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528042_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4064759
Sequences flagged as poor quality0
Sequence length59
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTTCATAGGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACC46450.11427491765194442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA900.053.00000452
CTATACG457.6397555E-1153.00000435
CGCGATA307.0149144E-753.00000433
TACGATA150.006697481553.00000439
GCGATAA457.6397555E-1153.00000431
ACTCGAT307.0149144E-753.00000425
TTTATCG457.6397555E-1153.00000428
CGTAATC457.6397555E-1153.00000425
CGTAACT600.053.00000452
TTACGCG150.006697481553.00000424
TCGACTA307.0149144E-753.00000443
CGATAAC307.0149144E-753.00000439
ATTACCG353.3449396E-853.016
CGTTAGT203.1391933E-453.043
CTACGTA650.053.027
TCGTAAT503.6379788E-1253.033
TTAGTCG700.053.025
CGTAAGA800.053.025
CACGTAA251.4794023E-553.050
TAATCGT203.1391933E-453.052