##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528042_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4064759
Sequences flagged as poor quality	0
Sequence length	59
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.404950945431207	31.0	31.0	33.0	30.0	34.0
2	31.911250826924796	31.0	31.0	34.0	30.0	34.0
3	32.11347314810054	33.0	31.0	34.0	30.0	34.0
4	35.72892833252845	37.0	35.0	37.0	35.0	37.0
5	35.49790061354191	37.0	35.0	37.0	33.0	37.0
6	35.81452110690941	37.0	35.0	37.0	35.0	37.0
7	35.7814918424438	37.0	35.0	37.0	33.0	37.0
8	35.84490593415256	37.0	35.0	37.0	35.0	37.0
9	37.836679616183886	39.0	38.0	39.0	35.0	39.0
10	37.466824724417854	39.0	37.0	39.0	35.0	39.0
11	37.49998979029261	39.0	37.0	39.0	35.0	39.0
12	37.65545189764018	39.0	37.0	39.0	35.0	39.0
13	37.741949522714634	39.0	37.0	39.0	35.0	39.0
14	38.244685355269525	40.0	38.0	40.0	35.0	40.0
15	37.71824356622373	40.0	38.0	40.0	33.0	40.0
16	37.115268826515916	39.0	36.0	40.0	32.0	40.0
17	36.98585918623958	39.0	36.0	40.0	32.0	40.0
18	36.940708907957394	39.0	36.0	40.0	32.0	40.0
19	37.0704949543134	40.0	36.0	40.0	32.0	40.0
20	37.17783046916188	40.0	36.0	40.0	32.0	40.0
21	37.10358400092109	40.0	36.0	40.0	32.0	40.0
22	37.08960998671754	39.0	36.0	40.0	32.0	40.0
23	37.012054097180176	39.0	35.0	40.0	31.0	40.0
24	36.975003930122305	39.0	35.0	40.0	31.0	40.0
25	36.91399785325526	39.0	35.0	40.0	31.0	40.0
26	36.77248097611691	39.0	35.0	40.0	31.0	40.0
27	36.667949563553464	39.0	35.0	40.0	31.0	40.0
28	36.649410703069975	39.0	35.0	40.0	31.0	40.0
29	36.638737991600486	39.0	35.0	40.0	31.0	40.0
30	36.60804244482883	39.0	35.0	40.0	31.0	40.0
31	36.60699293611257	39.0	35.0	40.0	31.0	40.0
32	36.49213003772179	39.0	35.0	40.0	30.0	40.0
33	36.33538642758402	39.0	35.0	40.0	30.0	40.0
34	36.2386094722959	39.0	35.0	40.0	30.0	40.0
35	36.08891252839344	38.0	35.0	40.0	30.0	40.0
36	35.926433276856024	38.0	35.0	40.0	30.0	40.0
37	35.75752141762894	38.0	35.0	40.0	29.0	40.0
38	35.511821488063624	38.0	34.0	40.0	28.0	40.0
39	35.36690588544118	38.0	34.0	40.0	28.0	40.0
40	35.20744206483091	38.0	34.0	40.0	27.0	40.0
41	35.081335449407945	37.0	34.0	40.0	27.0	40.0
42	34.963168788112654	37.0	34.0	40.0	27.0	40.0
43	34.74152169907244	37.0	33.0	40.0	26.0	40.0
44	34.58213266764401	36.0	33.0	40.0	26.0	40.0
45	34.373486103358154	36.0	33.0	40.0	25.0	40.0
46	34.162718134088635	36.0	33.0	39.0	25.0	40.0
47	34.0326110354882	35.0	33.0	39.0	24.0	40.0
48	33.84493250399347	35.0	33.0	39.0	24.0	40.0
49	33.691745070248935	35.0	33.0	39.0	24.0	40.0
50	33.48934733891972	35.0	33.0	39.0	24.0	40.0
51	33.30570840731271	35.0	32.0	38.0	23.0	40.0
52	33.143757354372056	35.0	32.0	38.0	23.0	40.0
53	32.89952786868791	35.0	32.0	38.0	23.0	40.0
54	32.69670674202333	35.0	31.0	37.0	23.0	40.0
55	32.55581647029996	35.0	31.0	37.0	22.0	40.0
56	32.350684259509606	35.0	31.0	37.0	22.0	40.0
57	32.19203131108142	35.0	31.0	37.0	21.0	39.0
58	31.99546049347575	35.0	31.0	36.0	21.0	39.0
59	31.47256282598796	34.0	30.0	36.0	20.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	6.0
14	20.0
15	88.0
16	363.0
17	956.0
18	2182.0
19	4177.0
20	7001.0
21	10855.0
22	15742.0
23	21877.0
24	29905.0
25	38899.0
26	49406.0
27	62267.0
28	76941.0
29	91535.0
30	109499.0
31	132932.0
32	162679.0
33	208375.0
34	301303.0
35	429416.0
36	480294.0
37	734478.0
38	915783.0
39	177776.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.360742174382295	18.855435217684494	34.637871519566104	16.14595108836711
2	24.103692248421122	22.66252439566528	35.97590902683283	17.257874329080764
3	22.09151883297386	22.37862072511556	36.27041603204519	19.25944440986538
4	22.676916392829195	22.57233454677141	35.63736497046934	19.113384089930054
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.59458851065955	0.32366002510849967	0.04391404262835755	0.03783742160359323
12	99.41125660832536	0.5002018569858632	0.05173738467643469	0.03680415001233776
13	98.4734150290337	0.9361440616774575	0.2424497983767303	0.34799111091211066
14	66.59610077743847	22.05156566477865	3.873587585389441	7.478745972393443
15	36.34412273888808	23.911011698356532	17.1717191597337	22.573146403021678
16	30.97029860810936	15.492456010307128	28.313683541877882	25.223561839705628
17	23.307433478836014	15.564145377376617	30.059666514054094	31.068754629733274
18	23.465278015252565	17.62862201670505	32.50797894782938	26.398121020213004
19	25.66809003928646	16.83536465507549	30.094108900429273	27.402436405208768
20	29.045018413145772	17.30105032057251	25.915878407551347	27.738052858730374
21	32.81269566043153	17.51565098939445	21.846166033459795	27.825487316714227
22	31.85527112431512	18.803894646644487	21.88594698972313	27.454887239317266
23	29.719720160531043	20.314168687491684	21.075050205928566	28.891060946048707
24	30.108525499297745	21.487030350384856	21.24996832530539	27.154475825012014
25	31.64605822879044	20.16614023119206	21.73540915955903	26.452392380458473
26	30.509508681818527	19.508831888926256	22.219226281312128	27.76243314794309
27	31.171958780336055	21.253756987806657	20.773039680827328	26.801244551029964
28	31.486048742373164	19.934638191341726	19.283504876918904	29.295808189366205
29	30.27662894651319	20.353334601141174	19.726286355476425	29.64375009686921
30	29.79276262135098	19.02781936149228	20.333800847725534	30.8456171694312
31	28.27318913618249	18.267749699305664	21.01332452920333	32.44573663530851
32	30.619945733560094	21.654051322599937	19.24175578429127	28.484247159548698
33	32.749346271205745	20.821013004707044	19.468140669594433	26.961500054492777
34	29.579613453097714	20.746592848432098	20.894301482572523	28.779492215897672
35	32.064016587453274	21.98430460452883	19.259690426910918	26.691988381106974
36	34.82831331451631	20.77813223366994	19.75583300264542	24.637721449168325
37	34.09646180745279	20.671410039315987	20.602746681906602	24.62938147132462
38	31.67617071516417	22.8813319559659	22.221071409153655	23.221425919716275
39	31.10826496724652	24.884328935614633	22.17804302788923	21.829363069249617
40	31.51478353329189	24.491365908778356	19.819600620848615	24.17424993708114
41	31.45930668952329	24.98160407541997	18.94646152453319	24.61262771052355
42	32.79702437463082	24.133878539908515	18.871327918826182	24.19776916663448
43	32.41928980291328	22.904801982110133	18.046900197527087	26.629008017449497
44	30.463232875553015	24.214695139367425	18.597510947143483	26.724561037936073
45	29.10128251146009	23.749968940348	20.553912298367504	26.594836249824404
46	28.88941263184361	22.12180353127947	20.871471100746685	28.117312736130234
47	29.54903353433746	19.881818331664928	22.718985307616023	27.850162826381585
48	29.402604188833827	18.79939253471116	21.862476963578896	29.935526312876114
49	30.166979149317342	20.03552978171646	21.879771961880152	27.917719107086054
50	29.792172180441696	21.621060436793424	20.27190295906842	28.314864423696463
51	27.977624257674314	21.378733646939462	22.6340848252012	28.00955727018502
52	30.06958100098924	20.822292293343835	22.353428579652572	26.754698126014357
53	29.70208073836604	20.743665245590208	21.815807530040527	27.738446486003227
54	27.20729568468881	21.40291712251575	22.804746849690225	28.585040343105213
55	27.087411578398623	21.335188629879408	22.88630150028575	28.69109829143622
56	27.16003581024115	22.342800643285372	22.65595574054944	27.84120780592404
57	27.999126147454252	20.62700396259655	23.178545148679174	28.19532474127002
58	28.571484803896123	20.350997439208573	23.084369823647602	27.993147933247702
59	27.200431809118324	20.60427198758893	22.396653774553425	29.79864242873932
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.5
4	5.0
5	5.0
6	47.0
7	89.0
8	173.0
9	383.5
10	510.0
11	712.5
12	915.0
13	1015.0
14	1300.0
15	1485.0
16	1648.5
17	1812.0
18	2281.0
19	3772.0
20	4794.0
21	6617.0
22	8440.0
23	10527.5
24	12615.0
25	15035.0
26	18824.0
27	20193.0
28	21268.5
29	22344.0
30	24897.0
31	27590.0
32	27730.0
33	31917.5
34	36105.0
35	38052.0
36	44825.0
37	49651.0
38	54541.5
39	59432.0
40	66077.5
41	72723.0
42	81776.0
43	95276.5
44	99724.0
45	111528.5
46	123333.0
47	136804.0
48	164880.0
49	179485.0
50	198533.0
51	217581.0
52	241292.5
53	299328.0
54	333652.0
55	351995.0
56	370338.0
57	365017.5
58	340134.0
59	320571.0
60	291661.0
61	262751.0
62	235694.0
63	208637.0
64	195516.5
65	164550.5
66	146705.0
67	137218.0
68	127731.0
69	107809.0
70	71936.5
71	55986.0
72	48603.0
73	41220.0
74	29336.0
75	12460.0
76	7468.0
77	5830.5
78	4193.0
79	2945.0
80	1697.0
81	1206.5
82	692.0
83	668.0
84	643.0
85	618.0
86	414.0
87	134.0
88	58.0
89	30.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	4064759.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.75775667661035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.21379678265515	9.431343046775293
2	7.0584588918032845	1.6598328432335954
3	3.0608978992602704	1.0796787813435011
4	1.7655800019331025	0.8303704022327462
5	1.0775618027847504	0.6334854740576342
6	0.7115255409086718	0.5019566507518636
7	0.469691362821849	0.3865761730015367
8	0.3308376031164135	0.3111926429532621
9	0.24061218133675016	0.25461535334273594
>10	1.7132151535103137	4.379965361332978
>50	0.7598548196018429	6.727081179079115
>100	2.443004424081481	61.535759519783994
>500	0.1462838845691339	10.938563199631341
>1k	0.008679651616981898	1.3295793724804366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCGTTCATAGGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACC	4645	0.11427491765194442	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.018869507392689208	0.0	0.0	0.0	0.0
11	0.020099592620374297	0.0	0.0	0.0	0.0
12	0.026004001713262705	0.0	0.0	0.0	0.0
13	0.031662393760614096	0.0	0.0	0.0	0.0
14	0.046103594333637	0.0	0.0	0.0	0.0
15	0.06507150854454102	0.0	0.0	0.0	0.0
16	0.07518280911611241	0.0	0.0	0.0	0.0
17	0.0835227869598173	0.0	0.0	0.0	0.0
18	0.09528240173648671	0.0	0.0	0.0	0.0
19	0.10785387276342828	0.0	0.0	0.0	0.0
20	0.12121259833608831	0.0	0.0	0.0	0.0
21	0.13663786709125927	0.0	0.0	0.0	0.0
22	0.1556795864158244	0.0	0.0	0.0	0.0
23	0.17159688926206942	0.0	0.0	0.0	0.0
24	0.1886704722223384	0.0	0.0	0.0	0.0
25	0.20822882734253126	0.0	0.0	0.0	0.0
26	0.23287973530534037	0.0	0.0	0.0	0.0
27	0.2550212694036719	0.0	0.0	0.0	0.0
28	0.2751946671377073	0.0	0.0	0.0	0.0
29	0.30348662737446425	0.0	0.0	0.0	0.0
30	0.3389376836363484	0.0	0.0	0.0	0.0
31	0.36973901773758294	0.0	0.0	0.0	0.0
32	0.3931352387681533	0.0	0.0	0.0	0.0
33	0.41247217854736284	0.0	0.0	0.0	0.0
34	0.4300869990078133	0.0	0.0	0.0	0.0
35	0.4479478365138007	0.0	0.0	0.0	0.0
36	0.4653166399287141	0.0	0.0	0.0	0.0
37	0.48253783311630527	0.0	0.0	0.0	0.0
38	0.5030802564186462	0.0	0.0	0.0	0.0
39	0.5259106382444814	0.0	0.0	0.0	0.0
40	0.5544486155267754	0.0	0.0	0.0	0.0
41	0.5781400570119901	0.0	0.0	0.0	0.0
42	0.5995681416782643	0.0	0.0	0.0	0.0
43	0.6176257928206814	0.0	0.0	0.0	0.0
44	0.6357572490767596	0.0	0.0	0.0	0.0
45	0.6540855189692673	0.0	0.0	0.0	0.0
46	0.6723645854526676	0.0	0.0	0.0	0.0
47	0.6895857786402588	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGTA	90	0.0	53.000004	52
CTATACG	45	7.6397555E-11	53.000004	35
CGCGATA	30	7.0149144E-7	53.000004	33
TACGATA	15	0.0066974815	53.000004	39
GCGATAA	45	7.6397555E-11	53.000004	31
ACTCGAT	30	7.0149144E-7	53.000004	25
TTTATCG	45	7.6397555E-11	53.000004	28
CGTAATC	45	7.6397555E-11	53.000004	25
CGTAACT	60	0.0	53.000004	52
TTACGCG	15	0.0066974815	53.000004	24
TCGACTA	30	7.0149144E-7	53.000004	43
CGATAAC	30	7.0149144E-7	53.000004	39
ATTACCG	35	3.3449396E-8	53.0	16
CGTTAGT	20	3.1391933E-4	53.0	43
CTACGTA	65	0.0	53.0	27
TCGTAAT	50	3.6379788E-12	53.0	33
TTAGTCG	70	0.0	53.0	25
CGTAAGA	80	0.0	53.0	25
CACGTAA	25	1.4794023E-5	53.0	50
TAATCGT	20	3.1391933E-4	53.0	52
>>END_MODULE