FastQCFastQC Report
Fri 17 Jun 2016
SRR1528041_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528041_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432099
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC14600.3378855308621404No Hit
GCCTTAGAGAGGGGGCAGCACCACATCGAGTCGCTGCACGAAGGCGCACAGA14390.3330255335004247No Hit
TTGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12970.30016269419739455No Hit
GTTTTAGAGAGGGGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCG11450.26498557043640464No Hit
TGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC11420.26429128509901667No Hit
TGGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC11420.26429128509901667No Hit
TTTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC11070.25619128949615716No Hit
TTGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9620.22263416485573906No Hit
GCCTTAGAGATCGGGGGACTCGCGTCGCGGCTGCGGAGACTAGAAGGAGGAC9180.21245131324071567No Hit
GTTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC8970.20759131587899993No Hit
TCTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC8920.20643417365002No Hit
TTATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC8750.20249989007148825No Hit
ATTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC8690.20111131939671234No Hit
TTGATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC8670.20064846250512036No Hit
TATTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC8210.19000275399850497No Hit
GTGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC8210.19000275399850497No Hit
TTGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC8070.18676275575736115No Hit
GGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC8030.18583704197417722No Hit
TGCGTAGAGAGGGCCAGCACTTTGGGAGGCCCAGGCGGGCGGATCAGCTGAG7880.1823656152872374No Hit
TAGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC7460.172645620563806No Hit
GGCGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC7430.17195133522641803No Hit
TGCGTAGAGAGGGGTAGAGAGGGCGTATCACCTGAGGTCAGGAGATCAAGAC7400.17125704988903007No Hit
TGTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC7150.1654713387441304No Hit
TGATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC7140.1652399102983344No Hit
TTTATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC7100.16431419651515045No Hit
GCTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC7090.16408276806935448No Hit
TGTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6990.16176848361139462No Hit
GGTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6940.16061134138241467No Hit
TCGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6860.1587599138160468No Hit
TCATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6850.1585284853702508No Hit
TGAGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6770.1566770578038829No Hit
TGGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6690.154825630237515No Hit
GTGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6570.15204848888796316No Hit
TTCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6530.15112277510477923No Hit
TAGCTAGAGAGGGGATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTG6400.1481142053094314No Hit
TGCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6390.14788277686363543No Hit
GTGATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6360.14718849152624747No Hit
GTGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6330.1464942061888595No Hit
TGGATAGAGAGGGGAACTCAGACAATTCCAAAGGATCTTGTATTAGTCTGTT6240.14441135017669562No Hit
GTCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6230.14417992173089964No Hit
TTTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6060.14024563815236785No Hit
TGGATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5980.13839421058599996No Hit
TTAGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5930.13723706835702004No Hit
TTGGTAGAGAGGAGTTCGAGACCAGCCCAGCCAACATGGTGAAACCCCGTCT5860.13561706923644815No Hit
GGAGTAGAGAGGGGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA5840.13515421234485617No Hit
TCTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5750.13307135633269226No Hit
TTCGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5730.1326084994411003No Hit
AGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5580.1291370727541605No Hit
GCTCTAGAGAGGGTGCGTGTGCGGTGTTGTGGGCCCTTTCTTGTCACGTCTG5570.12890564430836451No Hit
GTTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5540.12821135897097655No Hit
GGATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5530.12797993052518056No Hit
AATTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5510.1275170736335886No Hit
GAGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5380.12450850383824076No Hit
AGCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5360.12404564694664881No Hit
GGTGTAGAGAGGGGGTAGGCTATTCATGCCCTTCCCCCTGCATAGCACAGAG5280.12219421938028091No Hit
GTATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5280.12219421938028091No Hit
TATGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5230.12103707715130098No Hit
TTGATAGAGAGGGCAAGTAGCTGGGATTACAGACATGTGCCACCACACCCGG5220.12080564870550498No Hit
GGGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5180.11987993492232105No Hit
TGGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5090.11779707891015716No Hit
GTTATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5070.11733422201856518No Hit
ATTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5030.11640850823538124No Hit
GATTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4990.1154827944521973No Hit
GGCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4940.11432565222321736No Hit
GTTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4900.11339993844003342No Hit
GGCATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4860.11247422465684948No Hit
GCGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4800.11108565398207355No Hit
AAATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4750.10992851175309361No Hit
AGTTTAGAGAGGGGGGGATATCAGCCTCGGAGATCTCGTCAGCACCGAGCAG4740.10969708330729763No Hit
GTGTTAGAGAGGGGCAACTGCCATCACTCACACCATGCCTACTACTGAGGGC4710.10900279796990968No Hit
TCTATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4700.10877136952411369No Hit
TGCGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4700.10877136952411369No Hit
TCGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4670.10807708418672574No Hit
GCTATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4610.10668851351194981No Hit
TATATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4530.10483708594558191No Hit
TTCATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4520.10460565749978593No Hit
GGTCTAGAGAGGGCCCATCTCTACAAAAAATACAAAACTTAGCCGGGCATGG4510.10437422905398995No Hit
GTCTTAGAGAAGGACCCCGTCTCTACTATAAACACAAAAATTAGCGGGAGAA4480.10367994371660198No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTAC351.01757905E-746.00000444
TTCACAG351.01757905E-746.00000424
CATTGAA351.01757905E-746.00000415
AATCTAC351.01757905E-746.00000420
TCGACCG351.01757905E-746.00000431
ACGTCTG700.046.00000446
GCTAGGT351.01757905E-746.00000415
GATATGT351.01757905E-746.00000415
TTACCAG351.01757905E-746.00000438
CGGGGCT351.01757905E-746.00000412
CACGTCT700.046.00000445
TATGTCC351.01757905E-746.00000417
ATCTACA351.01757905E-746.00000421
GGTCTAG3350.046.0000041
CCTCGCC351.01757905E-746.00000444
AGGGCTT700.046.00000410
TGTCATT351.01757905E-746.00000446
ACCGCGC351.01757905E-746.00000434
GGATCAT351.01757905E-746.00000425
GGATTCA351.01757905E-746.00000421
Produced by FastQC (version 0.11.5)