FastQCFastQC Report
Fri 17 Jun 2016
SRR1528040_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528040_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429957
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTAGAGAGGGGGCAGCACCACATCGAGTCGCTGCACGAAGGCGCACAGA14530.33794077082126817No Hit
TTTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC14400.3349172126514977No Hit
TTGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12140.28235381677702653No Hit
TGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC11200.26049116539560935No Hit
GTTTTAGAGAGGGGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCG11180.26002600260026004No Hit
TGGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10840.2521182350793219No Hit
TTTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10360.2409543279909386No Hit
GCCTTAGAGATCGGGGGACTCGCGTCGCGGCTGCGGAGACTAGAAGGAGGAC9470.22025458359789468No Hit
TTGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9180.2135097230653298No Hit
TATTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9100.21164907188393256No Hit
GTTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9030.21002100210021002No Hit
TCTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC8850.2058345369420663No Hit
ATTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC8490.19746160662577886No Hit
TTGATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC8180.19025158329786468No Hit
GTGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC8110.1886235135141421No Hit
TTGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC7980.18559995534437165No Hit
GGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC7830.18211123437925186No Hit
TTATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC7750.18025058319785467No Hit
TTTATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC7360.17117990868854327No Hit
TGCGTAGAGAGGGCCAGCACTTTGGGAGGCCCAGGCGGGCGGATCAGCTGAG7360.17117990868854327No Hit
GGCGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC7310.17001700170017No Hit
TGCGTAGAGAGGGGTAGAGAGGGCGTATCACCTGAGGTCAGGAGATCAAGAC7250.16862151331412212No Hit
TGATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6950.16164407138388256No Hit
GGTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6900.16048116439550933No Hit
TAGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6810.15838793181643746No Hit
TGTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6570.15280597827224582No Hit
TCGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6510.15141048988619793No Hit
TGTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6480.15071274569317397No Hit
TCATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6450.15001500150015No Hit
GCTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6410.1490846759094514No Hit
TGGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6390.1486195131141021No Hit
TAGCTAGAGAGGGGATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTG6310.1467588619327049No Hit
TTCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6310.1467588619327049No Hit
GTCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6240.14513079214898233No Hit
GTGATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6230.14489821075130768No Hit
TGCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6060.14094432699083861No Hit
TTTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC6000.1395488386047907No Hit
GTGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5970.13885109441176677No Hit
TCTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5890.13699044323036955No Hit
GTGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5870.13652528043502024No Hit
TGAGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5860.1362926990373456No Hit
GCTCTAGAGAGGGTGCGTGTGCGGTGTTGTGGGCCCTTTCTTGTCACGTCTG5830.13559495484432163No Hit
TGGATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5780.1344320478559484No Hit
AGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5650.1314084896861779No Hit
GTTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5630.1309433268908286No Hit
TTAGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5610.13047816409547933No Hit
TGGATAGAGAGGGGAACTCAGACAATTCCAAAGGATCTTGTATTAGTCTGTT5510.1281523501187328No Hit
GATTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5430.1262916989373356No Hit
TTCGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5420.12605911753966095No Hit
TTGGTAGAGAGGAGTTCGAGACCAGCCCAGCCAACATGGTGAAACCCCGTCT5370.12489621055128768No Hit
GGGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5350.12443104775593837No Hit
GTATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5330.12396588496058909No Hit
GGATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5270.12257039657454116No Hit
GAGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5270.12257039657454116No Hit
GGTGTAGAGAGGGGGTAGGCTATTCATGCCCTTCCCCCTGCATAGCACAGAG5160.12001200120012002No Hit
TGGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC5050.11745360582569886No Hit
TTGATAGAGAGGGCAAGTAGCTGGGATTACAGACATGTGCCACCACACCCGG4900.11396488486057907No Hit
GTTATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4900.11396488486057907No Hit
AATTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4890.11373230346290444No Hit
GTGTTAGAGAGGGGCAACTGCCATCACTCACACCATGCCTACTACTGAGGGC4850.11280197787220583No Hit
ATTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4830.11233681507685651No Hit
GGAGTAGAGAGGGGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA4760.11070874529313396No Hit
AGCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4700.10931325690708607No Hit
TATGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4700.10931325690708607No Hit
GGCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4660.10838293131638745No Hit
AGTTTAGAGAGGGGGGGATATCAGCCTCGGAGATCTCGTCAGCACCGAGCAG4590.1067548615326649No Hit
GGCATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4590.1067548615326649No Hit
TATATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4580.10652228013499024No Hit
GCGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4560.10605711733964093No Hit
TGCGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4550.10582453594196628No Hit
TCGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4540.10559195454429163No Hit
GGTCTAGAGAGGGCCCATCTCTACAAAAAATACAAAACTTAGCCGGGCATGG4480.10419646615824373No Hit
GCGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4460.10373130336289442No Hit
AAATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4460.10373130336289442No Hit
GACTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4410.10256839637452118No Hit
GTTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4410.10256839637452118No Hit
GGACTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC4320.10047516379544932No Hit
AATTTAGAGAGGTGGTGTGGTGGCTCACACCTGTAATCCCAGCACTTTGAGA4320.10047516379544932No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGAG551.8189894E-1246.00000441
CCTACTA351.01757905E-746.00000438
AAACTAG1100.046.0000041
GGCACAC351.01757905E-746.00000422
CACACCC551.8189894E-1246.00000444
ACATGTG551.8189894E-1246.00000433
ACAGAAC351.01757905E-746.00000419
ACGTTGA700.046.00000425
CGGGGCT351.01757905E-746.00000412
ATGATAG1400.046.0000041
ACCACAC551.8189894E-1246.00000442
GTACTAG1450.046.0000041
GCCTACT351.01757905E-746.00000437
GGCAACT351.01757905E-746.00000413
CACAAAG351.01757905E-746.00000426
CCAGATG551.8189894E-1246.00000421
TAGCCGG700.046.00000440
TAGCTGG551.8189894E-1246.00000418
GCGGTAA351.01757905E-746.00000420
CCTGATT351.01757905E-746.00000419
Produced by FastQC (version 0.11.5)