FastQCFastQC Report
Fri 17 Jun 2016
SRR1528039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445754
Sequences flagged as poor quality0
Sequence length59
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTAGAGAGGGGGCAGCACCACATCGAGTCGCTGCACGAAGGCGCACAGACGGAGAT14370.3223751217038995No Hit
TTTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC12380.2777316636530463No Hit
GTTTTAGAGAGGGGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGGCAATCG10950.24565118877228248No Hit
TTGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC10800.24228610399457998No Hit
TGGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC10650.23892101921687744No Hit
TGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC9930.2227686122839055No Hit
TTTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC9750.21873051055066248No Hit
GCCTTAGAGATCGGGGGACTCGCGTCGCGGCTGCGGAGACTAGAAGGAGGACTCCGGAT9470.2124490189656178No Hit
TTGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC8710.19539925609192516No Hit
GTTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC8420.1888934255217003No Hit
ATTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC7830.1756574253960705No Hit
TATTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC7790.1747600694553498No Hit
TCTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC7790.1747600694553498No Hit
TTGATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC7680.17229234061836796No Hit
GTGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC7360.16511349309260265No Hit
TGCGTAGAGAGGGGTAGAGAGGGCGTATCACCTGAGGTCAGGAGATCAAGACCATCCTG7320.164216137151882No Hit
TAGCTAGAGAGGGGATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACA7220.16197274730008032No Hit
GCTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC7110.15950501846309847No Hit
TTGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC7100.15928067947791832No Hit
GGCGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC7010.1572616286112968No Hit
GGTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC6980.15658861165575633No Hit
TTATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC6880.15434522180395466No Hit
TAGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC6720.150755798041072No Hit
TGATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC6710.15053145905589183No Hit
GTGATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC6680.14985844210035132No Hit
TGTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC6550.14694203529300914No Hit
GGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC6300.141333560663505No Hit
TCGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC6260.1404362047227843No Hit
TTCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC6200.13909017081170333No Hit
TTTATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC6120.13729545893026196No Hit
TTTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC6110.1370711199450818No Hit
GTGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC6110.1370711199450818No Hit
TGGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC6020.13505206907846032No Hit
GTCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC5970.13393037415255948No Hit
GTGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC5700.12787322155269498No Hit
TGCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC5640.126527187641614No Hit
GCTCTAGAGAGGGTGCGTGTGCGGTGTTGTGGGCCCTTTCTTGTCACGTCTGCCGGCGT5590.12540549271571316No Hit
TGTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC5550.12450813677499248No Hit
TGGATAGAGAGGGGAACTCAGACAATTCCAAAGGATCTTGTATTAGTCTGTTTTCATGA5500.12338644184909164No Hit
TCATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC5440.12204040793801064No Hit
GGAGTAGAGAGGGGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTG5360.12024569605656932No Hit
AGCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC5350.12002135707138917No Hit
TTGGTAGAGAGGAGTTCGAGACCAGCCCAGCCAACATGGTGAAACCCCGTCTCTACTAA5340.11979701808620899No Hit
TGAGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC5290.11867532316030815No Hit
GGTGTAGAGAGGGGGTAGGCTATTCATGCCCTTCCCCCTGCATAGCACAGAGCAAGCAG5230.11732928924922716No Hit
TGGATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC5180.11620759432332632No Hit
GTTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC5160.11575891635296598No Hit
GGATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC5140.11531023838260565No Hit
AGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC5130.1150858993974255No Hit
GATTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC5060.11351552650116431No Hit
TCTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC5040.11306684853080398No Hit
TTGATAGAGAGGGCAAGTAGCTGGGATTACAGACATGTGCCACCACACCCGGCTAATTT5030.11284250954562382No Hit
GTATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC4950.11104779766418248No Hit
GAGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC4920.11037478070864198No Hit
GGGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC4920.11037478070864198No Hit
TTAGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC4900.10992610273828166No Hit
TATGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC4880.10947742476792133No Hit
ATTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC4830.10835572984202049No Hit
GGCATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC4820.10813139085684031No Hit
ATGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC4780.10723403491611964No Hit
GGTCTAGAGAGGGCCCATCTCTACAAAAAATACAAAACTTAGCCGGGCATGGTGGCATG4760.10678535694575932No Hit
AATTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC4720.10588800100503866No Hit
TGGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC4690.10521498404949817No Hit
TGCGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC4690.10521498404949817No Hit
GCGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC4640.1040932891235973No Hit
GCGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC4590.10297159419769648No Hit
GTCTTAGAGAAGGACCCCGTCTCTACTATAAACACAAAAATTAGCGGGAGAATCACTTG4590.10297159419769648No Hit
GTTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC4540.10184989927179565No Hit
GTGTTAGAGAGGGGCAACTGCCATCACTCACACCATGCCTACTACTGAGGGCTCAAGAA4510.10117688231625514No Hit
AGGTTAGAGAGGGTTCGCGCTATTACGTTGAGGTGGAAGCCCGTGCGCAAGGCTACCAG4460.10005518739035432No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAG203.1361563E-453.00000451
GCGAGAG203.1361563E-453.00000434
AGCACCG550.053.00000441
TCACGAT203.1361563E-453.00000419
TCTGCCG750.053.00000449
ACAACTG203.1361563E-453.00000450
CTTAAGC800.053.00000448
GAAATTT203.1361563E-453.00000417
CGGGTTA203.1361563E-453.00000427
TGATATG203.1361563E-453.00000414
CGGCGTG203.1361563E-453.00000422
AGCGTAT550.053.00000440
CTATGCC203.1361563E-453.00000428
GGAATAC800.053.00000433
AATCCTC203.1361563E-453.00000423
AACAAAT203.1361563E-453.00000435
ACTATAC203.1361563E-453.00000437
ACTATAA401.5970727E-953.00000425
TGATACT203.1361563E-453.00000435
GATACGG203.1361563E-453.00000423
Produced by FastQC (version 0.11.5)