FastQCFastQC Report
Fri 17 Jun 2016
SRR1528038_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528038_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415582
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG1050.046.00000423
TGTCACG1050.046.00000446
TCGTTTA351.0201984E-746.00000432
CTCAATA453.110472E-1046.00000446
CAACGAC453.110472E-1046.00000438
CTATACT700.046.00000426
GTTAGTC351.0201984E-746.00000441
TAAGTCC351.0201984E-746.00000440
CATTCCG453.110472E-1046.00000416
TTATCCG1150.046.00000421
TTATACG453.110472E-1046.00000427
TTAAGTC750.046.00000436
TAATTCG900.046.00000434
CCTCATA453.110472E-1046.00000441
CGCTAAA453.110472E-1046.00000438
TATTACT351.0201984E-746.00000423
ACGTCTT351.0201984E-746.00000441
AGTTACG351.0201984E-746.00000429
CGACTTA453.110472E-1046.00000437
ATACGTA453.110472E-1046.00000438
Produced by FastQC (version 0.11.5)