FastQCFastQC Report
Fri 17 Jun 2016
SRR1528037_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528037_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397696
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATAC206.312832E-446.00000425
AAACTAG206.312832E-446.00000444
ATTAGCG206.312832E-446.00000420
AGATAGT551.8189894E-1246.00000418
CATGATA206.312832E-446.00000435
ATAATCG551.8189894E-1246.00000426
ATTACTA351.0201984E-746.00000442
CTATACT700.046.00000426
CACGCAT206.312832E-446.00000419
CGCGCAT405.6152203E-946.00000431
TAAGTCC405.6152203E-946.00000440
CGACAAT800.046.00000431
CCTAAAT700.046.00000445
TCGATAA206.312832E-446.00000430
TCTAGAT700.046.00000437
GTAATAT206.312832E-446.00000423
ACGATTA351.0201984E-746.00000441
AATGCGC351.0201984E-746.00000444
TACGCTT351.0201984E-746.00000429
TAATTCG800.046.00000434
Produced by FastQC (version 0.11.5)