##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528037_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2397696
Sequences flagged as poor quality	0
Sequence length	52
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.724829169335894	31.0	31.0	34.0	30.0	34.0
2	32.187156753817	34.0	31.0	34.0	30.0	34.0
3	32.43871741872197	34.0	31.0	34.0	30.0	34.0
4	36.03703847360132	37.0	35.0	37.0	35.0	37.0
5	35.99376985239163	37.0	35.0	37.0	35.0	37.0
6	35.277966848174245	36.0	35.0	37.0	33.0	37.0
7	36.0008041052744	37.0	35.0	37.0	35.0	37.0
8	35.727280689461885	37.0	35.0	37.0	33.0	37.0
9	37.30454861667201	39.0	37.0	39.0	33.0	39.0
10	37.845882880899	39.0	38.0	39.0	35.0	39.0
11	38.04335578822336	39.0	37.0	39.0	37.0	39.0
12	37.99051506112535	39.0	38.0	39.0	35.0	39.0
13	38.04278273809524	39.0	39.0	39.0	37.0	39.0
14	38.72063264066838	40.0	39.0	40.0	36.0	40.0
15	38.121531253336535	40.0	38.0	40.0	34.0	40.0
16	37.515385186445656	40.0	37.0	40.0	33.0	40.0
17	37.514192374679695	40.0	37.0	40.0	33.0	40.0
18	37.59616314995729	40.0	37.0	40.0	33.0	40.0
19	37.695860943172114	40.0	37.0	40.0	33.0	40.0
20	37.720148008755075	40.0	37.0	40.0	33.0	40.0
21	37.72851937860346	40.0	37.0	40.0	33.0	40.0
22	37.64770179372197	40.0	37.0	40.0	33.0	40.0
23	37.558529938741195	40.0	37.0	40.0	33.0	40.0
24	37.483829893364295	40.0	37.0	40.0	33.0	40.0
25	37.39595845344864	40.0	36.0	40.0	33.0	40.0
26	37.36518891469144	40.0	36.0	40.0	33.0	40.0
27	37.337941507180226	40.0	36.0	40.0	33.0	40.0
28	37.117769725603246	40.0	35.0	40.0	32.0	40.0
29	37.19262742232543	40.0	35.0	40.0	32.0	40.0
30	37.20848514573991	40.0	35.0	40.0	33.0	40.0
31	37.14926245862695	40.0	35.0	40.0	33.0	40.0
32	37.008849328688875	39.0	35.0	40.0	32.0	40.0
33	36.83277196108264	39.0	35.0	40.0	32.0	40.0
34	36.726685117713004	39.0	35.0	40.0	32.0	40.0
35	36.51391502509075	39.0	35.0	40.0	31.0	40.0
36	36.001590276665596	38.0	35.0	40.0	30.0	40.0
37	36.00617092408712	38.0	35.0	40.0	31.0	40.0
38	35.870473988362164	37.0	35.0	40.0	30.0	40.0
39	35.7090440155883	37.0	35.0	40.0	30.0	40.0
40	35.50088334801409	37.0	35.0	40.0	30.0	40.0
41	35.29718112721546	36.0	35.0	40.0	30.0	40.0
42	34.97024852191437	36.0	34.0	40.0	29.0	40.0
43	34.71422315422806	35.0	34.0	39.0	28.0	40.0
44	34.54238151959214	35.0	34.0	39.0	28.0	40.0
45	34.109601884475765	35.0	33.0	39.0	26.0	40.0
46	34.0922005959054	35.0	33.0	39.0	26.0	40.0
47	33.98591064088191	35.0	33.0	38.0	27.0	40.0
48	33.802494561445656	35.0	33.0	38.0	26.0	40.0
49	33.66099622304078	35.0	33.0	37.0	26.0	40.0
50	33.432198243647235	35.0	33.0	37.0	26.0	40.0
51	33.36668660247171	35.0	33.0	37.0	26.0	40.0
52	32.883366782110826	35.0	33.0	36.0	24.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	4.0
14	7.0
15	17.0
16	61.0
17	159.0
18	405.0
19	852.0
20	1553.0
21	2645.0
22	4243.0
23	6658.0
24	9681.0
25	13687.0
26	18740.0
27	24883.0
28	32755.0
29	41692.0
30	50773.0
31	61549.0
32	73943.0
33	93239.0
34	137136.0
35	241360.0
36	313687.0
37	532119.0
38	563899.0
39	171946.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.44701580183643	21.167570868033312	33.60104867339312	17.784364656737132
2	26.167245555733505	23.316175194853724	33.66402579809951	16.85255345131326
3	17.985390975336323	24.78149857196242	35.257597293401666	21.975513159299595
4	21.54063734518471	25.28118660580824	36.2356612347854	16.942514814221653
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	0.0	100.0
11	98.65554265428145	1.0332836189408499	0.11523562620115312	0.19593810057655348
12	98.87617112427931	0.8184940876574845	0.14113549007046766	0.1641992979927397
13	97.04829135970532	1.63957399103139	0.5130758861840701	0.7990587630792227
14	62.97637398569294	22.417604233397395	5.3878389920990815	9.21818278881059
15	36.15433315983344	24.730491271620757	17.66024550234892	21.45493006619688
16	28.074409766709373	17.992773062139655	26.90099161862054	27.03182555253043
17	23.343743326927182	17.29827300875507	30.282612975122785	29.075370689194962
18	24.06973194266496	18.488415545590435	29.6407467835789	27.801105728165705
19	25.80685791693359	16.951815409459748	27.812867019004912	29.428459654601753
20	29.28619808349349	17.049450806107195	24.699711723254325	28.96463938714499
21	30.667399036408284	18.432320027226137	21.742456091180866	29.15782484518471
22	30.94099502188768	18.731357102818706	21.950238896006834	28.377408979286784
23	30.72032484518471	18.276837430600043	21.40646687486654	29.59637084934871
24	29.884480768204142	20.505810578155028	22.17020005872304	27.43950859491779
25	31.627362267777066	18.007578942451417	23.297657417787743	27.067401371983774
26	30.90229119955157	18.205852618513774	21.44049954623105	29.451356635703608
27	30.880311765961988	20.494341234251547	19.770980140935297	28.854366858851165
28	30.62594257153534	20.129532684710654	20.876708306641042	28.367816437112964
29	29.938490951313263	20.03148022101217	20.580382166880202	29.449646660794365
30	30.863754204035875	19.96587557388426	19.66646313794576	29.503907084134102
31	31.339377469036943	19.771647448216957	19.80196822282725	29.08700685991886
32	31.412739563314112	21.057089806213966	19.798923633354686	27.731246997117232
33	30.392802090006406	20.73715767136451	21.14300561872731	27.727034619901776
34	30.43659413036515	21.371349829169336	20.87587417253897	27.31618186792654
35	31.663688807922274	21.87474975976938	19.366675341661328	27.09488609064702
36	32.68045657164211	20.766435778347216	21.11255972400171	25.440547926008968
37	32.50061725923553	21.15409960228486	20.4925895526372	25.852693585842406
38	31.488937713538327	22.622634395686525	20.881879938073887	25.006547952701258
39	32.08342508808456	22.68723808189195	21.954784926863123	23.27455190316037
40	32.18710795697203	22.67977258167841	19.63009489109545	25.503024570254112
41	32.107364736814006	23.14759669282511	19.195469317211188	25.54956925314969
42	32.855040839205635	22.35733804452274	19.451173126201155	25.336447990070464
43	31.658517176489433	21.414015788490286	19.944813687806963	26.982653347213326
44	31.33516509182148	22.55498612000854	20.324511531069827	25.78533725710015
45	29.184183482810166	21.29665312032885	21.231548953662184	28.287614443198805
46	29.05368320254111	19.790749119154388	21.275924887892376	29.87964279041213
47	30.19073310377963	18.850304625774076	24.058012358530856	26.90094991191544
48	29.494856729126628	19.724894231795858	23.8176149103139	26.962634128763614
49	30.89807882233611	19.718179452274185	22.849643991565234	26.534097733824467
50	29.492354326820415	20.620295483664318	21.99490677717275	27.892443412342516
51	28.74463651772368	22.184505458573565	22.11769131699765	26.953166706705105
52	30.440889920990816	21.797008461456333	21.956828555413196	25.805273062139655
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	19.5
10	36.0
11	55.5
12	75.0
13	114.5
14	277.5
15	401.0
16	593.0
17	785.0
18	910.5
19	1036.0
20	1297.0
21	1558.0
22	1932.0
23	2306.0
24	3033.5
25	3761.0
26	4684.5
27	5608.0
28	7096.0
29	8584.0
30	10249.0
31	11914.0
32	12225.0
33	12536.0
34	13332.5
35	14129.0
36	14334.0
37	14539.0
38	16314.5
39	19027.0
40	19964.0
41	22398.0
42	24832.0
43	26232.5
44	27633.0
45	32516.5
46	37400.0
47	44174.5
48	50949.0
49	60342.5
50	69736.0
51	80808.5
52	91881.0
53	105463.0
54	119045.0
55	130918.0
56	142791.0
57	163349.5
58	183908.0
59	203842.0
60	223776.0
61	239653.0
62	255530.0
63	260080.5
64	242880.5
65	221130.0
66	193731.5
67	166333.0
68	146229.5
69	126126.0
70	114414.0
71	102702.0
72	87448.5
73	72195.0
74	60420.5
75	48646.0
76	40223.5
77	31801.0
78	22116.0
79	12431.0
80	8763.5
81	5096.0
82	3605.5
83	2115.0
84	1713.0
85	1311.0
86	751.5
87	192.0
88	108.0
89	13.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2397696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.754275851204971
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.68809883867169	7.577911464280803
2	7.663665264442362	1.49507010041337
3	3.5606084975860623	1.0419347245079684
4	2.1233582035855094	0.8284728659476843
5	1.3612762424511333	0.6639131989280067
6	0.8699258764013599	0.5091298181112063
7	0.6167479014569198	0.42111504130238037
8	0.4148354116278589	0.3237135230293039
9	0.2883339798825582	0.25312402593452316
>10	1.8183226296109356	3.5544687174180742
>50	0.5652907459676197	4.164829376822906
>100	2.798760637224069	63.785553460812835
>500	0.2149511467885299	13.463983436051333
>1k	0.015824624303450052	1.916780246439602
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0062560057655349135	0.0	0.0	0.0	0.0
12	0.015640014413837285	0.0	0.0	0.0	0.0
13	0.01726657591287636	0.0	0.0	0.0	0.0
14	0.025149143177450352	0.0	0.0	0.0	0.0
15	0.0361597133247918	0.0	0.0	0.0	0.0
16	0.04863001815075806	0.0	0.0	0.0	0.0
17	0.06276859118086696	0.0	0.0	0.0	0.0
18	0.07653180386504378	0.0	0.0	0.0	0.0
19	0.0944656870595772	0.0	0.0	0.0	0.0
20	0.11164884956224642	0.0	0.0	0.0	0.0
21	0.12995809310271195	0.0	0.0	0.0	0.0
22	0.14814221652786674	0.0	0.0	0.0	0.0
23	0.16645146006833225	0.0	0.0	0.0	0.0
24	0.18546971759555841	0.0	0.0	0.0	0.0
25	0.20865864563314115	0.0	0.0	0.0	0.0
26	0.23359925528507366	0.0	0.0	0.0	0.0
27	0.2627105354473628	0.0	0.0	0.0	0.0
28	0.2885269859064702	0.0	0.0	0.0	0.0
29	0.31763826606875933	0.0	0.0	0.0	0.0
30	0.3523382447149263	0.0	0.0	0.0	0.0
31	0.3841187540038437	0.0	0.0	0.0	0.0
32	0.40768304238735853	0.0	0.0	0.0	0.0
33	0.42799420777279523	0.0	0.0	0.0	0.0
34	0.44788830610719627	0.0	0.0	0.0	0.0
35	0.46644778987828317	0.0	0.0	0.0	0.0
36	0.4834224188554346	0.0	0.0	0.0	0.0
37	0.5006472880632074	0.0	0.0	0.0	0.0
38	0.5187897047832586	0.0	0.0	0.0	0.0
39	0.5390591634635917	0.0	0.0	0.0	0.0
40	0.5616641976297245	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATAC	20	6.312832E-4	46.000004	25
AAACTAG	20	6.312832E-4	46.000004	44
ATTAGCG	20	6.312832E-4	46.000004	20
AGATAGT	55	1.8189894E-12	46.000004	18
CATGATA	20	6.312832E-4	46.000004	35
ATAATCG	55	1.8189894E-12	46.000004	26
ATTACTA	35	1.0201984E-7	46.000004	42
CTATACT	70	0.0	46.000004	26
CACGCAT	20	6.312832E-4	46.000004	19
CGCGCAT	40	5.6152203E-9	46.000004	31
TAAGTCC	40	5.6152203E-9	46.000004	40
CGACAAT	80	0.0	46.000004	31
CCTAAAT	70	0.0	46.000004	45
TCGATAA	20	6.312832E-4	46.000004	30
TCTAGAT	70	0.0	46.000004	37
GTAATAT	20	6.312832E-4	46.000004	23
ACGATTA	35	1.0201984E-7	46.000004	41
AATGCGC	35	1.0201984E-7	46.000004	44
TACGCTT	35	1.0201984E-7	46.000004	29
TAATTCG	80	0.0	46.000004	34
>>END_MODULE