FastQCFastQC Report
Fri 17 Jun 2016
SRR1528036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383199
Sequences flagged as poor quality0
Sequence length59
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGGTTGCCGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGTCT23840.10003361028600632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAC251.4792155E-553.00000413
TATTTCG600.053.00000423
CGTATAA307.0136775E-753.00000445
CGACGTA150.00669714353.00000436
CTATCGG150.00669714353.00000447
CCGATAC307.0136775E-753.00000421
TCATTAC150.00669714353.00000416
ACGCAAT251.4792155E-553.00000420
AGCTTAT150.00669714353.00000415
ATTACGT307.0136775E-753.00000420
CAACCGT307.0136775E-753.00000441
AAGGTAC150.00669714353.00000442
CAATTCG307.0136775E-753.00000428
CCTAAAT503.6379788E-1253.00000445
TTATACG503.6379788E-1253.00000427
GCATTAT150.00669714353.00000449
ATTCATA150.00669714353.00000429
CTAGTTT307.0136775E-753.00000418
AGCATTA150.00669714353.00000448
TACGATT600.053.00000440