##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528036_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2383199
Sequences flagged as poor quality	0
Sequence length	59
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.332479578918925	31.0	31.0	33.0	30.0	34.0
2	31.87953209110947	31.0	31.0	34.0	30.0	34.0
3	32.16466606439496	33.0	31.0	34.0	30.0	34.0
4	35.8710426615654	37.0	35.0	37.0	35.0	37.0
5	35.70877169720195	37.0	35.0	37.0	33.0	37.0
6	34.89566125195588	35.0	35.0	37.0	32.0	37.0
7	35.71399199143672	37.0	35.0	37.0	35.0	37.0
8	35.33495818016036	37.0	35.0	37.0	32.0	37.0
9	36.8672825894942	39.0	37.0	39.0	32.0	39.0
10	37.55996792546489	39.0	37.0	39.0	35.0	39.0
11	37.563259719393976	39.0	37.0	39.0	35.0	39.0
12	37.62865837053473	39.0	37.0	39.0	35.0	39.0
13	37.66757077356947	39.0	37.0	39.0	35.0	39.0
14	38.104078173916655	40.0	38.0	40.0	34.0	40.0
15	37.44039209482716	40.0	37.0	40.0	32.0	40.0
16	36.83217935220684	39.0	36.0	40.0	31.0	40.0
17	36.72773528354116	39.0	36.0	40.0	31.0	40.0
18	36.770120329859154	39.0	36.0	40.0	31.0	40.0
19	36.94198302365854	39.0	36.0	40.0	31.0	40.0
20	37.08098022867583	39.0	36.0	40.0	31.0	40.0
21	37.060169125616454	39.0	36.0	40.0	31.0	40.0
22	36.989708370975315	39.0	36.0	40.0	31.0	40.0
23	36.919955488400255	39.0	35.0	40.0	31.0	40.0
24	36.82811968282967	39.0	35.0	40.0	31.0	40.0
25	36.717395819652495	39.0	35.0	40.0	31.0	40.0
26	36.53286695739634	39.0	35.0	40.0	30.0	40.0
27	36.38340944251823	39.0	35.0	40.0	30.0	40.0
28	36.310776817210815	39.0	35.0	40.0	30.0	40.0
29	36.23315174267864	39.0	35.0	40.0	30.0	40.0
30	36.18689584881498	39.0	35.0	40.0	30.0	40.0
31	36.10625172299921	38.0	35.0	40.0	30.0	40.0
32	35.892990472050386	38.0	35.0	40.0	29.0	40.0
33	35.65994195197296	38.0	34.0	40.0	29.0	40.0
34	35.52727615276777	38.0	34.0	40.0	28.0	40.0
35	35.337906737960196	37.0	34.0	40.0	28.0	40.0
36	35.11106122484946	37.0	34.0	40.0	27.0	40.0
37	34.887802906933075	37.0	33.0	40.0	27.0	40.0
38	34.634526113849496	36.0	33.0	40.0	26.0	40.0
39	34.395011914657566	36.0	33.0	39.0	26.0	40.0
40	34.12461275789391	36.0	33.0	39.0	25.0	40.0
41	33.89558320559886	35.0	33.0	39.0	24.0	40.0
42	33.726640116918475	35.0	33.0	39.0	24.0	40.0
43	33.42599002433284	35.0	33.0	39.0	23.0	40.0
44	33.24868422653752	35.0	32.0	38.0	23.0	40.0
45	33.01544017096348	35.0	32.0	38.0	23.0	40.0
46	32.79734969677312	35.0	32.0	38.0	23.0	40.0
47	32.650020413738005	35.0	32.0	37.0	23.0	40.0
48	32.430709311308036	35.0	31.0	37.0	22.0	40.0
49	32.28368885686844	35.0	31.0	37.0	22.0	39.0
50	32.07911760620913	35.0	31.0	36.0	21.0	39.0
51	31.929886677528817	35.0	31.0	36.0	21.0	39.0
52	31.73916697682401	34.0	31.0	36.0	20.0	39.0
53	31.550202899548044	34.0	31.0	35.0	20.0	39.0
54	31.340526326169154	34.0	30.0	35.0	20.0	38.0
55	31.158919586656424	34.0	30.0	35.0	20.0	38.0
56	30.96596885111147	34.0	30.0	35.0	18.0	37.0
57	30.832488180802358	34.0	30.0	35.0	18.0	37.0
58	30.679140516591353	34.0	30.0	35.0	18.0	37.0
59	30.174220868672737	33.0	29.0	35.0	18.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	6.0
14	20.0
15	116.0
16	330.0
17	936.0
18	1885.0
19	3702.0
20	6424.0
21	9387.0
22	13037.0
23	17465.0
24	23175.0
25	30215.0
26	37975.0
27	47047.0
28	55101.0
29	63328.0
30	73565.0
31	88362.0
32	108063.0
33	137784.0
34	193942.0
35	276546.0
36	384188.0
37	444281.0
38	322784.0
39	43529.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.522166634007483	21.395737410094583	33.10269096286127	17.97940499303667
2	25.681111816512175	23.698608467022687	33.62912622907277	16.991153487392367
3	18.024638311781768	25.00408064958067	35.008238925914284	21.963042112723276
4	21.509450113062318	25.52833397462822	36.065389419851215	16.896826492458246
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	0.0	100.0
11	98.60758585413974	1.0623955448118263	0.12210478436756646	0.2079138166808563
12	98.84726369891898	0.8243121954985715	0.14409203763512823	0.18433206794732626
13	97.02043345939639	1.6777449134545623	0.5115812821338042	0.7902403450152504
14	62.80159567035737	22.53328404384191	5.414109354695096	9.251010931105627
15	36.12241361296308	24.91180971458951	17.586907345966495	21.37886932648092
16	28.120312235780563	18.196340297222346	26.790880660826055	26.892466806171033
17	23.29398426232975	17.478901258350646	30.15765783721796	29.069456642101642
18	24.0192279369033	18.631385796989676	29.569666653938675	27.779719612168353
19	25.708260199840634	17.204564117390113	27.75962057721575	29.327555105553504
20	29.244515460102154	17.28928217912142	24.64389251589985	28.82230984487657
21	30.696009859017227	18.55250023183125	21.662479717388265	29.089010191763254
22	30.76604177829883	18.95590758472121	21.930522797298924	28.347527839681035
23	30.541847323702303	18.522456580419846	21.487882463864747	29.447813632013105
24	29.84706690461015	20.669990210637046	22.150395329974543	27.332547554778262
25	31.612047504215973	18.1585339705161	23.311313910420406	26.918104614847522
26	30.7476211596262	18.393554210118417	21.47227319246106	29.386551437794328
27	30.793861528139278	20.668983160869068	19.802458795929336	28.734696515062318
28	30.446093674930207	20.29662650915849	20.855329328352354	28.401950487558945
29	29.818533827850718	20.20620183207529	20.617791464330086	29.357472875743905
30	30.757691657305998	20.18874630276364	19.6988585510484	29.35470348888196
31	31.31060394033398	19.996441757486473	19.77254102573893	28.920413276440616
32	31.27304937606973	21.354112686351414	19.716356040767053	27.65648189681181
33	30.422511926196677	20.966566367307134	21.115399930933172	27.495521775563013
34	30.372746883495672	21.61670091335218	20.901359894830435	27.10919230832171
35	31.46728410006886	22.120267757749144	19.38331629041469	27.029131851767307
36	32.57298278490382	20.97693058783593	21.06832035428011	25.381766272980144
37	32.35441102484518	21.420158366968096	20.428885712019852	25.79654489616687
38	31.41202224405096	22.92422915585312	20.7878150334907	24.875933566605223
39	31.982096333541598	22.93220163318296	21.85902226377235	23.226679769503093
40	32.07831154679068	22.988680341003835	19.59462890006248	25.338379212143007
41	31.98797079052148	23.37882820528206	19.145065099473438	25.48813590472302
42	32.64620369511736	22.586196117067857	19.35528673853925	25.41231344927553
43	31.65837179354305	21.618673052481142	19.940508534956585	26.782446619019222
44	30.964682345032873	22.867204962741255	20.381554372924796	25.786558319301072
45	29.138313669987276	21.40891297789232	21.12089674425006	28.331876607870342
46	28.98906050229125	19.99157435027457	21.24686188606155	29.772503261372634
47	30.17884784275254	19.082502132637686	23.86103720251645	26.877612822093326
48	29.442820343580205	19.882393371262744	23.691643039460825	26.98314324569623
49	30.747369397184205	19.946592793971465	22.749380139887602	26.556657668956724
50	29.253201264350988	20.844125899683576	21.946215989516613	27.95645684644883
51	28.697603515275055	22.331076842512942	22.063663168707272	26.907656473504733
52	30.209017375384935	22.104071040647465	21.885834963844815	25.801076620122785
53	28.293314993838116	21.510121479574305	22.528626438665004	27.667937087922578
54	28.065553904646656	22.472063810030132	21.795536167982615	27.666846117340597
55	27.747955584069985	22.127149264497007	22.715266328997284	27.409628822435728
56	28.90362911364095	20.927123584727923	22.18329228906189	27.985955012569242
57	29.684092683825398	20.852266218641414	21.80845997333836	27.655181124194833
58	27.97831821849539	22.281101997776936	21.50118391288348	28.239395870844184
59	29.30720430815891	21.026863472164933	21.902031680946493	27.763900538729665
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	10.0
9	38.5
10	58.0
11	83.5
12	109.0
13	189.0
14	373.5
15	478.0
16	518.0
17	558.0
18	623.5
19	724.5
20	760.0
21	976.5
22	1193.0
23	1409.0
24	1625.0
25	2399.0
26	3855.5
27	4538.0
28	5882.5
29	7227.0
30	8484.0
31	11353.0
32	12965.0
33	13757.0
34	14549.0
35	15357.5
36	17077.5
37	17989.0
38	18635.5
39	19282.0
40	21565.5
41	23849.0
42	22918.5
43	26738.5
44	31489.0
45	34465.5
46	37442.0
47	41516.5
48	49376.0
49	53161.0
50	61287.5
51	69414.0
52	78065.5
53	103229.0
54	119741.0
55	122191.0
56	124641.0
57	145625.5
58	187365.0
59	208120.0
60	211780.5
61	215441.0
62	222274.5
63	229108.0
64	212964.0
65	182979.5
66	169139.0
67	151091.5
68	133044.0
69	116850.5
70	89983.0
71	79309.0
72	70528.0
73	61747.0
74	53260.5
75	35002.5
76	25231.0
77	21057.5
78	16884.0
79	11957.5
80	7031.0
81	4719.5
82	1618.5
83	829.0
84	633.5
85	438.0
86	272.5
87	80.5
88	54.0
89	35.5
90	17.0
91	10.5
92	2.5
93	1.0
94	1.0
95	1.0
96	2.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	2383199.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.806924410290094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.1234100086785	11.476786387153751
2	6.426747764215202	1.774672411690403
3	2.818291659335033	1.1673581971976954
4	1.5505002460232697	0.8563055877991792
5	0.9405151076694213	0.6492810499163774
6	0.592408755038456	0.49076057404860135
7	0.3858418650677921	0.37291026257214477
8	0.2675434919307663	0.2955162215642519
9	0.2120581456863524	0.2635083709269976
>10	1.2207582013713743	3.4745455947333705
>50	0.39820335706641813	4.1363670086093585
>100	1.9073604311530274	60.356336642206784
>500	0.14540961500655802	12.79681365383429
>1k	0.010951351757816086	1.8888380377468004
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATGGTTGCCGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGTCT	2384	0.10003361028600632	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.005790536165884595	0.0	0.0	0.0	0.0
12	0.013804973902724867	0.0	0.0	0.0	0.0
13	0.015357508961693925	0.0	0.0	0.0	0.0
14	0.02160960960456932	0.0	0.0	0.0	0.0
15	0.03105070117938116	0.0	0.0	0.0	0.0
16	0.04208628822016122	0.0	0.0	0.0	0.0
17	0.05756967840285264	0.0	0.0	0.0	0.0
18	0.07162641474757249	0.0	0.0	0.0	0.0
19	0.08761332981425386	0.0	0.0	0.0	0.0
20	0.10351632406693692	0.0	0.0	0.0	0.0
21	0.12025852645960325	0.0	0.0	0.0	0.0
22	0.13977011571421438	0.0	0.0	0.0	0.0
23	0.15814877397984808	0.0	0.0	0.0	0.0
24	0.1765274322454818	0.0	0.0	0.0	0.0
25	0.19578725905809796	0.0	0.0	0.0	0.0
26	0.21957880982662378	0.0	0.0	0.0	0.0
27	0.24450329158412704	0.0	0.0	0.0	0.0
28	0.2707285459586044	0.0	0.0	0.0	0.0
29	0.29905182068303987	0.0	0.0	0.0	0.0
30	0.32943115535043443	0.0	0.0	0.0	0.0
31	0.35834187577285825	0.0	0.0	0.0	0.0
32	0.3807067727034125	0.0	0.0	0.0	0.0
33	0.39828818323606213	0.0	0.0	0.0	0.0
34	0.4153241084777226	0.0	0.0	0.0	0.0
35	0.4318565088353931	0.0	0.0	0.0	0.0
36	0.4469202949480929	0.0	0.0	0.0	0.0
37	0.4605993876298203	0.0	0.0	0.0	0.0
38	0.4788102042674573	0.0	0.0	0.0	0.0
39	0.49681121887009855	0.0	0.0	0.0	0.0
40	0.5159871248687163	0.0	0.0	0.0	0.0
41	0.5349532288323384	0.0	0.0	0.0	0.0
42	0.5558075511109227	0.0	0.0	0.0	0.0
43	0.5737666053065649	0.0	0.0	0.0	0.0
44	0.5922711447931961	0.0	0.0	0.0	0.0
45	0.6099784365468431	0.0	0.0	0.0	0.0
46	0.6285249364404735	0.0	0.0	0.0	0.0
47	0.646861634299108	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAC	25	1.4792155E-5	53.000004	13
TATTTCG	60	0.0	53.000004	23
CGTATAA	30	7.0136775E-7	53.000004	45
CGACGTA	15	0.006697143	53.000004	36
CTATCGG	15	0.006697143	53.000004	47
CCGATAC	30	7.0136775E-7	53.000004	21
TCATTAC	15	0.006697143	53.000004	16
ACGCAAT	25	1.4792155E-5	53.000004	20
AGCTTAT	15	0.006697143	53.000004	15
ATTACGT	30	7.0136775E-7	53.000004	20
CAACCGT	30	7.0136775E-7	53.000004	41
AAGGTAC	15	0.006697143	53.000004	42
CAATTCG	30	7.0136775E-7	53.000004	28
CCTAAAT	50	3.6379788E-12	53.000004	45
TTATACG	50	3.6379788E-12	53.000004	27
GCATTAT	15	0.006697143	53.000004	49
ATTCATA	15	0.006697143	53.000004	29
CTAGTTT	30	7.0136775E-7	53.000004	18
AGCATTA	15	0.006697143	53.000004	48
TACGATT	60	0.0	53.000004	40
>>END_MODULE