FastQCFastQC Report
Fri 17 Jun 2016
SRR1528035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5185709
Sequences flagged as poor quality0
Sequence length52
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAA1400.046.00000417
ATGAGTC206.3135143E-446.00000424
CTACGGT551.8189894E-1246.00000444
CGTAACG750.046.00000417
CCTATAA405.6188583E-946.00000428
GATTCGC405.6188583E-946.00000445
GCGTAAC206.3135143E-446.00000416
CTAATTG750.046.00000438
CGACGTT301.8626779E-646.030
ACTTATA501.6370905E-1146.027
CCGTACT650.046.015
TGCACGA301.8626779E-646.036
ATTCTAC650.046.032
CTTAGGT1450.045.9999968
CCGCAAT1450.044.4137933
CTAGCAC1250.044.1616
CCAGTAC1100.043.90909243
TCATTAC800.043.12500421
GTCTAGG1600.043.12500431
TCTAGGA1600.043.12500432
Produced by FastQC (version 0.11.5)