##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528035_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5185709
Sequences flagged as poor quality	0
Sequence length	52
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.064806953109017	31.0	31.0	34.0	27.0	34.0
2	31.84677331489291	33.0	31.0	34.0	30.0	34.0
3	32.01990065389323	33.0	31.0	34.0	30.0	34.0
4	35.68677764217005	37.0	35.0	37.0	33.0	37.0
5	35.62617705698488	37.0	35.0	37.0	33.0	37.0
6	35.724778810380606	37.0	35.0	37.0	33.0	37.0
7	35.66655167114082	37.0	35.0	37.0	33.0	37.0
8	35.55826021089884	37.0	35.0	37.0	33.0	37.0
9	37.904929489873034	39.0	38.0	39.0	35.0	39.0
10	38.04754335424529	39.0	38.0	39.0	35.0	39.0
11	38.30742199379101	39.0	39.0	39.0	37.0	39.0
12	38.26066965963574	39.0	39.0	39.0	37.0	39.0
13	38.17238259994921	39.0	39.0	39.0	37.0	39.0
14	38.679415485905594	40.0	39.0	40.0	36.0	40.0
15	37.756222341053075	40.0	37.0	40.0	33.0	40.0
16	36.44177180015308	38.0	35.0	40.0	31.0	40.0
17	36.06664527454202	38.0	35.0	40.0	31.0	40.0
18	35.94005043476215	38.0	35.0	40.0	29.0	40.0
19	36.25412976316257	39.0	35.0	40.0	30.0	40.0
20	36.33610042522633	39.0	35.0	40.0	31.0	40.0
21	36.36465447636957	39.0	35.0	40.0	31.0	40.0
22	36.548763534552364	39.0	35.0	40.0	31.0	40.0
23	36.55209615502914	39.0	35.0	40.0	31.0	40.0
24	36.744795745384096	39.0	35.0	40.0	32.0	40.0
25	36.70439027720221	39.0	35.0	40.0	31.0	40.0
26	36.59912135447631	39.0	35.0	40.0	31.0	40.0
27	36.494010751471016	39.0	35.0	40.0	31.0	40.0
28	36.20004111298957	38.0	35.0	40.0	30.0	40.0
29	35.941738342818695	38.0	35.0	40.0	30.0	40.0
30	35.69419090041497	38.0	35.0	40.0	30.0	40.0
31	35.4084772207619	37.0	35.0	40.0	29.0	40.0
32	35.10377134544187	37.0	34.0	40.0	27.0	40.0
33	34.68781395176629	35.0	34.0	40.0	25.0	40.0
34	34.31277227472656	35.0	33.0	40.0	23.0	40.0
35	33.986248360638825	35.0	33.0	40.0	22.0	40.0
36	33.6526449902993	35.0	33.0	40.0	21.0	40.0
37	33.41292752832833	35.0	33.0	40.0	20.0	40.0
38	33.074416246650166	35.0	33.0	40.0	18.0	40.0
39	32.64962225994555	35.0	33.0	40.0	16.0	40.0
40	32.0924801218117	35.0	31.0	40.0	15.0	40.0
41	31.444675935344616	35.0	30.0	39.0	10.0	40.0
42	30.710633782188705	35.0	27.0	39.0	10.0	40.0
43	29.93022593439007	35.0	23.0	38.0	9.0	40.0
44	29.187143551633923	35.0	21.0	37.0	8.0	40.0
45	28.50253012654586	35.0	20.0	37.0	8.0	40.0
46	27.897148104531126	34.0	18.0	35.0	8.0	40.0
47	27.363308276650308	33.0	15.0	35.0	8.0	40.0
48	26.885350682037885	33.0	14.0	35.0	8.0	40.0
49	26.47640062332846	33.0	10.0	35.0	8.0	40.0
50	26.071007262459194	33.0	10.0	35.0	8.0	40.0
51	25.638023267406638	33.0	10.0	35.0	8.0	39.0
52	24.938565970439143	32.0	10.0	35.0	8.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	6.0
13	12.0
14	44.0
15	174.0
16	734.0
17	2493.0
18	6220.0
19	12318.0
20	20676.0
21	31162.0
22	43125.0
23	57125.0
24	75736.0
25	100316.0
26	132376.0
27	168124.0
28	207603.0
29	251669.0
30	291782.0
31	303184.0
32	267165.0
33	243130.0
34	282195.0
35	375459.0
36	522516.0
37	783362.0
38	834496.0
39	172507.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.68938827843984	23.539076334595713	36.41266796883512	2.358867418129324
2	29.94761179233158	21.21707947746393	31.249748105803853	17.585560624400635
3	17.96965467981331	22.640394977813063	35.3516751518452	24.03827519052843
4	14.10593999779008	25.948563639031807	32.90358174745247	27.04191461572564
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.48732179148502	0.30692042303183614	0.15023982255849683	0.055517962924645406
12	99.28572929950369	0.45951286506820954	0.14306626152759438	0.11169157390050231
13	96.69136852839216	2.135754243055289	0.5560088311935745	0.6168683973589725
14	59.056283335605606	27.730557190926064	5.560801811285593	7.652357662182741
15	43.06423673214213	34.495533783326444	13.58223147500178	8.85799800952965
16	45.92868593282037	31.124905003346697	14.642703630303977	8.303705433528954
17	30.89471468607282	33.461461103968624	19.283689077038453	16.36013513292011
18	24.11058931382382	44.42202599490253	17.94506787789288	13.522316813380774
19	26.7972807575589	44.3367338969464	17.92383645129335	10.942148894201353
20	19.088170971413938	48.25264973410579	20.272425622031626	12.386753672448647
21	20.778258093541307	49.46401736001769	16.11035250917473	13.647372037266264
22	22.881191366503597	50.00432920551462	16.893003444659158	10.221475983322629
23	17.083797027561708	47.11207281395852	20.891993746660294	14.912136411819482
24	18.787170664609217	50.008359512652945	16.84072515445815	14.36374466827969
25	18.386241881293376	43.84709978905488	22.686598881657265	15.08005944799448
26	19.484683772267207	44.34957688524366	21.992711122047147	14.173028220441989
27	24.528912054262975	46.21086528380208	18.38764959622686	10.872573065708083
28	27.280088412211327	44.396783544930884	17.672935369107677	10.650192673750109
29	31.367919025151625	41.95173697559967	16.825182438891193	9.855161560357512
30	35.303716425275695	43.03101080295867	12.886492473835304	8.778780297930332
31	31.90045951286507	45.78907146544474	11.934549354774825	10.375919666915363
32	30.005135266942283	50.516872427666115	12.465489289892664	7.012503015498941
33	28.430307215464655	53.10943980851992	11.727634543318956	6.732618432696475
34	25.114290061397583	55.20278904967479	12.891544820582876	6.791376068344753
35	25.512037023288425	55.468268659116816	11.965229826818282	7.054464490776478
36	20.28245318046192	57.64987969822448	13.409680334935878	8.657986786377716
37	19.06589822143896	59.16245975236944	13.996890299860636	7.774751726330961
38	18.27146490479894	60.30089617446718	13.443484777105697	7.98415414362819
39	19.235479661508194	60.67912796495138	11.794279239347985	8.29111313419245
40	19.52953781247656	61.08771240345341	11.412113560556522	7.970636223513507
41	20.215017850018192	60.84998213359061	11.349402752834761	7.58559726355644
42	21.850647616362583	57.814620912974476	11.95072843462678	8.384003036036153
43	23.326010001718185	56.7207107070605	10.323294268922533	9.629985022298783
44	21.80340238914293	55.69699341015857	11.489769287092662	11.009834913605834
45	21.977824054531407	54.80808892284546	13.735826672881183	9.478260349741955
46	23.233505775198722	54.40428685836402	12.725723714925	9.636483651512261
47	25.86865171184885	53.71340736628299	10.54715951087884	9.870781410989316
48	25.841384466424937	53.412792734802515	10.546098903737175	10.199723895035376
49	24.955603949238185	53.87855354012344	10.655167885432832	10.51067462520554
50	23.871374193962676	54.23707732153886	11.00410763504084	10.887440849457615
51	22.574926591522974	54.43716567975565	11.524981444195962	11.462926284525414
52	22.798926819842762	53.92047644786856	11.769191059506038	11.511405672782642
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	10.5
6	19.0
7	38.5
8	58.0
9	459.0
10	860.0
11	2023.5
12	3187.0
13	5673.5
14	11833.0
15	15506.0
16	19053.5
17	22601.0
18	28317.5
19	34034.0
20	44788.0
21	55542.0
22	73378.0
23	91214.0
24	118583.0
25	145952.0
26	180186.5
27	214421.0
28	249018.5
29	283616.0
30	307826.0
31	332036.0
32	345297.0
33	358558.0
34	359361.5
35	360165.0
36	354440.5
37	348716.0
38	338863.5
39	317788.0
40	306565.0
41	301107.0
42	295649.0
43	297539.0
44	299429.0
45	304588.0
46	309747.0
47	314054.5
48	318362.0
49	308912.0
50	299462.0
51	280757.0
52	262052.0
53	228481.0
54	194910.0
55	163081.5
56	131253.0
57	103707.0
58	76161.0
59	58399.0
60	40637.0
61	31787.0
62	22937.0
63	17176.5
64	8572.0
65	5728.0
66	4714.5
67	3701.0
68	2746.0
69	1791.0
70	1417.0
71	1043.0
72	780.0
73	517.0
74	443.0
75	369.0
76	253.0
77	137.0
78	134.0
79	131.0
80	81.0
81	31.0
82	23.5
83	16.0
84	9.5
85	3.0
86	3.5
87	4.0
88	2.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	5185709.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.38391123611164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.45095307083982	41.54201500854
2	6.786710644926515	6.83882053438303
3	2.512134915044166	3.797135477181663
4	1.4740797557479812	2.9707961427422167
5	1.0000273077019222	2.519264355247067
6	0.7815855272254094	2.3627601496292727
7	0.6151641728897446	2.169606395775905
8	0.5242495210497755	2.1130993067316743
9	0.47088804586877087	2.13526633546784
>10	3.2925328557863156	29.04862891345316
>50	0.07483032955002354	2.467271350730926
>100	0.015228883164578608	1.3922154992519946
>500	0.001307357883322274	0.44111419455089007
>1k	3.0761232160486424E-4	0.2020063363144429
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.012958690894533418	0.0	0.0	0.0	0.0
12	0.01961159023770906	0.0	0.0	0.0	0.0
13	0.033013807755120854	0.0	0.0	0.0	0.0
14	0.16904149461529755	0.0	0.0	0.0	0.0
15	0.3628433450469357	0.0	0.0	0.0	0.0
16	0.5050418370949855	0.0	0.0	0.0	0.0
17	0.6272430635810841	0.0	0.0	0.0	0.0
18	0.7796619517215486	0.0	0.0	0.0	0.0
19	0.9488384327003309	0.0	0.0	0.0	0.0
20	1.1330948188569778	0.0	0.0	0.0	0.0
21	1.302599123861366	0.0	0.0	0.0	0.0
22	1.4705221600363614	0.0	0.0	0.0	0.0
23	1.641781287766051	0.0	0.0	0.0	0.0
24	1.8386492570254136	0.0	0.0	0.0	0.0
25	2.0551481002886973	0.0	0.0	0.0	0.0
26	2.310484448703157	0.0	0.0	0.0	0.0
27	2.5920660029322895	0.0	0.0	0.0	0.0
28	2.8862205727317134	0.0	0.0	0.0	0.0
29	3.201837974325208	0.0	0.0	0.0	0.0
30	3.54759590250822	0.0	0.0	0.0	0.0
31	3.8943565865342618	0.0	0.0	0.0	0.0
32	4.228872079015618	0.0	0.0	0.0	0.0
33	4.544778737102294	0.0	0.0	0.0	0.0
34	4.854514590001097	0.0	0.0	0.0	0.0
35	5.153952911742637	0.0	0.0	0.0	0.0
36	5.442843013366157	0.0	0.0	0.0	0.0
37	5.7255430260355915	0.0	0.0	0.0	0.0
38	5.998022642612611	0.0	0.0	0.0	0.0
39	6.275342484508869	0.0	0.0	0.0	0.0
40	6.554783540688457	0.0	0.0	0.0	1.92837662121033E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCAA	140	0.0	46.000004	17
ATGAGTC	20	6.3135143E-4	46.000004	24
CTACGGT	55	1.8189894E-12	46.000004	44
CGTAACG	75	0.0	46.000004	17
CCTATAA	40	5.6188583E-9	46.000004	28
GATTCGC	40	5.6188583E-9	46.000004	45
GCGTAAC	20	6.3135143E-4	46.000004	16
CTAATTG	75	0.0	46.000004	38
CGACGTT	30	1.8626779E-6	46.0	30
ACTTATA	50	1.6370905E-11	46.0	27
CCGTACT	65	0.0	46.0	15
TGCACGA	30	1.8626779E-6	46.0	36
ATTCTAC	65	0.0	46.0	32
CTTAGGT	145	0.0	45.999996	8
CCGCAAT	145	0.0	44.41379	33
CTAGCAC	125	0.0	44.16	16
CCAGTAC	110	0.0	43.909092	43
TCATTAC	80	0.0	43.125004	21
GTCTAGG	160	0.0	43.125004	31
TCTAGGA	160	0.0	43.125004	32
>>END_MODULE