FastQCFastQC Report
Fri 17 Jun 2016
SRR1528034_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528034_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5154698
Sequences flagged as poor quality0
Sequence length52
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAA1150.046.00000419
CGTAACG1150.046.00000417
ATAACGT1100.046.00000435
CGAACCT206.3135114E-446.032
GATAAAC206.3135114E-446.037
ACGTTTT301.8626743E-646.032
CTATCAG253.4188248E-546.022
TGAACGT1500.046.043
CGATTAA405.6188583E-946.034
ACCCTTA206.3135114E-446.032
ACCTAAC253.4188248E-546.022
GGACGTA501.6370905E-1146.012
TACTAGA501.6370905E-1146.018
GTCTAGG1450.044.4137931
TAGCGGC1300.044.2307737
TCTATGA1050.043.80952527
CTATGAG1000.043.728
ATAAGTA1000.043.737
ATACTAT950.043.5789544
TCGTTTA900.043.44444719
Produced by FastQC (version 0.11.5)