##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528034_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5154698
Sequences flagged as poor quality	0
Sequence length	52
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.965157803619146	31.0	31.0	34.0	27.0	34.0
2	31.659439020481898	33.0	31.0	34.0	28.0	34.0
3	31.941412280603053	33.0	31.0	34.0	30.0	34.0
4	35.652724563107284	37.0	35.0	37.0	33.0	37.0
5	35.610801253536096	37.0	35.0	37.0	33.0	37.0
6	35.712241531899636	37.0	35.0	37.0	33.0	37.0
7	35.6671345246608	37.0	35.0	37.0	33.0	37.0
8	35.49487147452673	37.0	35.0	37.0	33.0	37.0
9	37.88751096572486	39.0	38.0	39.0	35.0	39.0
10	38.04722876102538	39.0	38.0	39.0	35.0	39.0
11	38.293107957051994	39.0	39.0	39.0	37.0	39.0
12	38.24922022589878	39.0	39.0	39.0	37.0	39.0
13	38.16982837791855	39.0	39.0	39.0	37.0	39.0
14	38.70427520681134	40.0	39.0	40.0	36.0	40.0
15	37.77236648975362	40.0	37.0	40.0	33.0	40.0
16	36.46384269262719	38.0	35.0	40.0	31.0	40.0
17	36.11518152954839	38.0	35.0	40.0	31.0	40.0
18	36.02613829170981	38.0	35.0	40.0	30.0	40.0
19	36.32716814835709	39.0	35.0	40.0	30.0	40.0
20	36.37371190319976	39.0	35.0	40.0	31.0	40.0
21	36.61556331719142	39.0	35.0	40.0	31.0	40.0
22	36.68416966425579	39.0	35.0	40.0	31.0	40.0
23	36.64959887077769	39.0	35.0	40.0	31.0	40.0
24	36.8325626447951	39.0	35.0	40.0	32.0	40.0
25	36.88752532156103	39.0	35.0	40.0	32.0	40.0
26	36.75379488769274	39.0	35.0	40.0	32.0	40.0
27	36.51362504651097	39.0	35.0	40.0	31.0	40.0
28	36.00812482127954	38.0	35.0	40.0	30.0	40.0
29	35.84820643226819	38.0	35.0	40.0	30.0	40.0
30	35.667576839613105	38.0	35.0	40.0	30.0	40.0
31	35.42833062189094	37.0	35.0	40.0	29.0	40.0
32	35.12967297017207	37.0	35.0	40.0	27.0	40.0
33	34.706212080707736	35.0	34.0	40.0	25.0	40.0
34	34.36593511394848	35.0	33.0	40.0	23.0	40.0
35	33.986335571938454	35.0	33.0	40.0	22.0	40.0
36	33.260772600063085	35.0	33.0	40.0	20.0	40.0
37	33.26886657569464	35.0	33.0	40.0	20.0	40.0
38	33.037248156924036	35.0	33.0	40.0	18.0	40.0
39	32.64302098784449	35.0	33.0	40.0	16.0	40.0
40	32.104499623450295	35.0	31.0	40.0	15.0	40.0
41	31.48246395812131	35.0	30.0	39.0	10.0	40.0
42	30.59679519537323	35.0	26.0	39.0	9.0	40.0
43	29.864776753167693	35.0	23.0	38.0	9.0	40.0
44	29.1364861724198	35.0	21.0	37.0	8.0	40.0
45	28.247791820199748	35.0	20.0	36.0	8.0	40.0
46	27.792028747367937	34.0	18.0	35.0	8.0	40.0
47	27.3238218805447	33.0	15.0	35.0	8.0	40.0
48	26.842575452528937	33.0	12.0	35.0	8.0	40.0
49	26.458046232776393	33.0	10.0	35.0	8.0	40.0
50	26.00444487727506	33.0	10.0	35.0	8.0	40.0
51	25.65083095071719	33.0	10.0	35.0	8.0	39.0
52	25.01656372497477	32.0	10.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	5.0
13	10.0
14	36.0
15	176.0
16	738.0
17	2473.0
18	5852.0
19	11600.0
20	20075.0
21	30206.0
22	41959.0
23	55825.0
24	74790.0
25	100323.0
26	131873.0
27	167865.0
28	207436.0
29	252426.0
30	293250.0
31	301682.0
32	266814.0
33	244633.0
34	283437.0
35	378628.0
36	524911.0
37	779368.0
38	806955.0
39	171349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.690588275006604	23.567297250003783	36.33654192738353	2.405572547606087
2	30.003096204666114	21.300898714143873	31.120950247715772	17.57505483347424
3	17.99432284878765	22.63052074049731	35.323485488383604	24.051670922331432
4	14.115977308466956	25.93050068112623	32.936478528907024	27.01704348149979
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.47178282801437	0.313810818790936	0.15711880696017497	0.05728754623452237
12	99.28228579055455	0.4592121594708361	0.14617733182428924	0.11232471815031647
13	96.69431652445982	2.1306000855918232	0.557394439014662	0.6176889509336919
14	59.08524611917129	27.72125544503286	5.556659187405353	7.636839248390498
15	43.101710323281786	34.430533078756504	13.623688526466534	8.844068071495167
16	45.978076698188715	31.071577811154015	14.664331450649485	8.286014040007775
17	30.889239292001196	33.45668359232685	19.28310834116761	16.370968774504348
18	24.1040503245777	44.46008282153484	17.9445624166537	13.491304437233762
19	26.797457387416294	44.36700268376537	17.920972285864273	10.914567642954058
20	19.064395237121552	48.29074370603283	20.294302401420993	12.350558655424624
21	20.378012446121964	49.84687754743343	16.05141562124493	13.723694385199675
22	22.87517134854457	50.01526762576586	16.917363539047294	10.192197486642282
23	17.094037322846074	47.128696967310205	20.86060133881752	14.916664371026197
24	18.736694176846054	50.07032419746026	16.817260681421104	14.375720944272585
25	18.397295050068887	43.89688784871587	22.668854703030128	15.03696239818511
26	19.502849633479983	44.401010495668224	21.918219069283982	14.177920801567812
27	24.51365725014346	46.338175388742464	18.32832883711131	10.819838524002764
28	27.391653206453608	44.27529993027719	17.650442373151638	10.682604490117559
29	31.359004931035727	42.01443033132106	16.779954907154597	9.846609830488614
30	35.25552806391373	43.08993465766569	12.865351180612327	8.789186097808251
31	31.835153097232855	45.86090203538597	11.93456920269626	10.369375664684915
32	29.96284166405093	50.59118885335281	12.473262255131145	6.97270722746512
33	28.437107275731773	53.12152137719804	11.738398641394705	6.702972705675483
34	25.15189056662485	55.244536149353465	12.846397596910625	6.757175687111058
35	25.54366909564828	55.41086985115326	11.991546352473026	7.053914700725435
36	20.55711120224696	56.722702280521574	13.546322209370947	9.17386430786052
37	19.05048559585838	59.19681424595582	13.979034271260895	7.773665886924898
38	18.299423166982816	60.30298962228243	13.426586775791716	7.971000434943036
39	19.19024160096285	60.7223740362675	11.781000555221665	8.306383807547988
40	19.516041482934597	61.13908128080442	11.391200803616428	7.95367643264455
41	20.197128910364874	60.84994698040506	11.37690316678106	7.576020942449005
42	22.032755362195807	57.51101228432781	12.066177300784643	8.39005505269174
43	23.270946231961602	56.73162617868205	10.364040725567241	9.633386863789111
44	22.073320299268744	55.542070553890845	11.442513218039156	10.942095928801262
45	22.18147406501797	54.350788348803356	13.861432813328733	9.606304772849933
46	23.350853920055066	54.3436686300536	12.75653782239037	9.548939627500971
47	25.855714534585733	53.667198349932434	10.591076334636869	9.886010780844968
48	26.002609658218578	53.32114509909213	10.56550742642925	10.110737816260041
49	25.07409745439985	53.7439244743339	10.671954011660818	10.510024059605431
50	24.103313132990525	53.99598579781008	11.071376053456477	10.82932501574292
51	22.55965334923598	54.44825283653862	11.503544921545355	11.488548892680036
52	22.852066212220386	53.87642496223833	11.78270773573932	11.488801089801964
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.5
4	5.0
5	15.5
6	26.0
7	38.5
8	51.0
9	479.5
10	908.0
11	2059.0
12	3210.0
13	5524.0
14	11500.5
15	15163.0
16	18913.5
17	22664.0
18	28393.5
19	34123.0
20	44252.0
21	54381.0
22	71827.0
23	89273.0
24	116287.0
25	143301.0
26	177904.0
27	212507.0
28	246399.0
29	280291.0
30	304513.0
31	328735.0
32	341745.0
33	354755.0
34	356911.0
35	359067.0
36	353458.0
37	347849.0
38	337412.5
39	317274.5
40	307573.0
41	301695.0
42	295817.0
43	297115.5
44	298414.0
45	303091.0
46	307768.0
47	312151.5
48	316535.0
49	307098.0
50	297661.0
51	279535.0
52	261409.0
53	227955.0
54	194501.0
55	162545.5
56	130590.0
57	102844.0
58	75098.0
59	57912.5
60	40727.0
61	31740.0
62	22753.0
63	16949.5
64	8537.5
65	5929.0
66	4818.5
67	3708.0
68	2713.5
69	1719.0
70	1355.0
71	991.0
72	767.5
73	544.0
74	448.0
75	352.0
76	259.5
77	167.0
78	139.5
79	112.0
80	70.0
81	28.0
82	23.0
83	18.0
84	12.0
85	6.0
86	6.5
87	7.0
88	4.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	5154698.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.98693295842469
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.31233297257019	41.96853402923967
2	6.875762284168424	7.011480612419209
3	2.5857609584180805	3.9552006190012396
4	1.4461712369144148	2.9494334361182957
5	1.036151201366874	2.6415085919442007
6	0.7885489654044728	2.412341594010781
7	0.6347745446424506	2.2655645005979435
8	0.5511702695452106	2.248198526558282
9	0.4738514751754571	2.1744210057319506
>10	3.2123966442085843	28.23830982033594
>50	0.06753283464481225	2.2347972175362987
>100	0.0141273036738796	1.3230212330395703
>500	0.0011507917649088706	0.3995593741784198
>1k	2.685175023690539E-4	0.17762943928829633
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.013948440820393357	0.0	0.0	0.0	0.0
12	0.021359156249308883	0.0	0.0	0.0	0.0
13	0.035792591534945406	0.0	0.0	0.0	0.0
14	0.17525759996026927	0.0	0.0	0.0	0.0
15	0.3685375942489744	0.0	0.0	0.0	0.0
16	0.5101753778785877	0.0	0.0	0.0	0.0
17	0.634004164744472	0.0	0.0	0.0	0.0
18	0.786505824395532	0.0	0.0	0.0	0.0
19	0.9603472405172913	0.0	0.0	0.0	0.0
20	1.141521772953527	0.0	0.0	0.0	0.0
21	1.3143932001448	0.0	0.0	0.0	0.0
22	1.4811342972953994	0.0	0.0	0.0	0.0
23	1.6492333789486795	0.0	0.0	0.0	0.0
24	1.8450159446780394	0.0	0.0	0.0	0.0
25	2.061517473962587	0.0	0.0	0.0	0.0
26	2.312065614707205	0.0	0.0	0.0	0.0
27	2.5933236049910198	0.0	0.0	0.0	0.0
28	2.8838934890075034	0.0	0.0	0.0	0.0
29	3.193184159382373	0.0	0.0	0.0	0.0
30	3.5413519860911347	0.0	0.0	0.0	0.0
31	3.886183050879023	0.0	0.0	0.0	0.0
32	4.214078108940621	0.0	0.0	0.0	0.0
33	4.525910150313365	0.0	0.0	0.0	0.0
34	4.825462131826152	0.0	0.0	0.0	0.0
35	5.117021404551731	0.0	0.0	0.0	0.0
36	5.391295474536045	1.9399778609726506E-5	0.0	0.0	0.0
37	5.664987551162066	1.9399778609726506E-5	0.0	0.0	0.0
38	5.934838471623362	1.9399778609726506E-5	0.0	0.0	0.0
39	6.209384138508211	1.9399778609726506E-5	0.0	0.0	0.0
40	6.481446633731016	1.9399778609726506E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGAA	115	0.0	46.000004	19
CGTAACG	115	0.0	46.000004	17
ATAACGT	110	0.0	46.000004	35
CGAACCT	20	6.3135114E-4	46.0	32
GATAAAC	20	6.3135114E-4	46.0	37
ACGTTTT	30	1.8626743E-6	46.0	32
CTATCAG	25	3.4188248E-5	46.0	22
TGAACGT	150	0.0	46.0	43
CGATTAA	40	5.6188583E-9	46.0	34
ACCCTTA	20	6.3135114E-4	46.0	32
ACCTAAC	25	3.4188248E-5	46.0	22
GGACGTA	50	1.6370905E-11	46.0	12
TACTAGA	50	1.6370905E-11	46.0	18
GTCTAGG	145	0.0	44.41379	31
TAGCGGC	130	0.0	44.23077	37
TCTATGA	105	0.0	43.809525	27
CTATGAG	100	0.0	43.7	28
ATAAGTA	100	0.0	43.7	37
ATACTAT	95	0.0	43.57895	44
TCGTTTA	90	0.0	43.444447	19
>>END_MODULE