FastQCFastQC Report
Fri 17 Jun 2016
SRR1528033_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528033_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5415120
Sequences flagged as poor quality0
Sequence length59
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTA203.1392864E-453.022
ACTAGGT150.00669760353.048
TAACCGT203.1392864E-453.016
CTACGCG150.00669760353.026
TACCTAA401.6025297E-953.021
CCGTCAA1150.050.69565217
TCTAGGA1200.048.58333232
TCAGCTA1400.047.32143444
TGGGCGC47550.046.59095410
TACTGGC1650.046.5757627
TAACGTC800.046.37536
ACGGATT800.046.37531
CTATCTA700.045.42857724
CGATTAA352.0301122E-645.42857734
TACTCTA700.045.42857752
CAGCTAA700.045.42857725
CATTTCG352.0301122E-645.42857729
TGGGCAG269650.045.4229610
CGCGATA1350.045.14814832
CTACGGT1350.045.14814844
Produced by FastQC (version 0.11.5)