##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528033_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5415120
Sequences flagged as poor quality	0
Sequence length	59
%GC	39
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.777361166511547	31.0	31.0	34.0	27.0	34.0
2	31.545237039991726	31.0	31.0	34.0	28.0	34.0
3	31.743198304008036	31.0	31.0	34.0	30.0	34.0
4	35.50224593360812	37.0	35.0	37.0	33.0	37.0
5	35.28098287757243	37.0	35.0	37.0	33.0	37.0
6	35.41129540988935	37.0	35.0	37.0	33.0	37.0
7	35.36977167634328	37.0	35.0	37.0	32.0	37.0
8	35.26594350633042	37.0	35.0	37.0	32.0	37.0
9	37.652663652883035	39.0	37.0	39.0	35.0	39.0
10	37.80347582325045	39.0	37.0	39.0	35.0	39.0
11	38.114163859711326	39.0	38.0	39.0	35.0	39.0
12	38.102321093530705	39.0	39.0	39.0	37.0	39.0
13	38.00806814992096	39.0	38.0	39.0	35.0	39.0
14	38.18251673093117	40.0	38.0	40.0	33.0	40.0
15	37.21164018525905	40.0	36.0	40.0	31.0	40.0
16	35.9923820709421	38.0	35.0	40.0	31.0	40.0
17	35.49955901254266	38.0	34.0	40.0	27.0	40.0
18	35.33154907001138	38.0	34.0	40.0	27.0	40.0
19	35.58393959875312	38.0	34.0	40.0	27.0	40.0
20	35.74313902554329	38.0	34.0	40.0	28.0	40.0
21	35.92150866462793	38.0	34.0	40.0	30.0	40.0
22	35.99072171992495	38.0	35.0	40.0	30.0	40.0
23	35.97690171224276	38.0	35.0	40.0	30.0	40.0
24	36.12971273028114	38.0	35.0	40.0	30.0	40.0
25	36.05522499963067	38.0	35.0	40.0	30.0	40.0
26	35.821952976111334	38.0	35.0	40.0	29.0	40.0
27	35.62101689343911	38.0	35.0	40.0	27.0	40.0
28	35.36913438668026	38.0	34.0	40.0	27.0	40.0
29	35.0887804887057	37.0	34.0	40.0	27.0	40.0
30	34.85611473060615	37.0	33.0	40.0	25.0	40.0
31	34.633322807250806	36.0	33.0	40.0	25.0	40.0
32	34.24544239093501	35.0	33.0	40.0	24.0	40.0
33	33.74878857716911	35.0	33.0	40.0	22.0	40.0
34	33.405478918288054	35.0	33.0	40.0	21.0	40.0
35	33.06384105984724	35.0	32.0	40.0	20.0	40.0
36	32.62560737342847	35.0	31.0	39.0	18.0	40.0
37	32.44589593582414	35.0	31.0	39.0	18.0	40.0
38	32.139180664509745	35.0	31.0	39.0	16.0	40.0
39	31.737007674806836	35.0	30.0	39.0	15.0	40.0
40	31.169819505384922	35.0	28.0	39.0	12.0	40.0
41	30.556091093087502	35.0	25.0	38.0	10.0	40.0
42	29.917646331013902	35.0	24.0	38.0	9.0	40.0
43	29.10458789463576	34.0	22.0	37.0	8.0	40.0
44	28.40446989171062	34.0	20.0	37.0	8.0	40.0
45	27.707341111554314	33.0	20.0	35.0	8.0	40.0
46	27.06319656812776	33.0	16.0	35.0	8.0	40.0
47	26.557278693731625	33.0	15.0	35.0	8.0	40.0
48	26.04342470711637	33.0	12.0	35.0	8.0	39.0
49	25.70238702004757	32.0	10.0	35.0	8.0	39.0
50	25.267729616333526	31.0	10.0	35.0	8.0	39.0
51	24.850332771942266	31.0	10.0	35.0	8.0	38.0
52	24.444424500288083	30.0	10.0	35.0	8.0	38.0
53	24.021716785592933	29.0	10.0	35.0	8.0	38.0
54	23.55215544623203	27.0	9.0	35.0	8.0	37.0
55	23.095842197402828	26.0	9.0	35.0	8.0	37.0
56	22.525893978342122	24.0	9.0	35.0	8.0	36.0
57	21.952907599462247	23.0	8.0	35.0	8.0	35.0
58	21.33910273456544	20.0	8.0	34.0	8.0	35.0
59	20.634489909734224	20.0	8.0	33.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	8.0
13	31.0
14	176.0
15	872.0
16	3455.0
17	9943.0
18	20804.0
19	36057.0
20	54357.0
21	75177.0
22	101183.0
23	132128.0
24	170102.0
25	211840.0
26	259976.0
27	305834.0
28	322993.0
29	282296.0
30	233255.0
31	233004.0
32	263645.0
33	309013.0
34	371350.0
35	430234.0
36	528990.0
37	570137.0
38	435576.0
39	52681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.95476369868073	23.941500834699877	35.70419492088818	2.399540545731212
2	29.515670936193473	21.746997296458805	30.950098982109353	17.78723278523837
3	17.89812598797441	22.899732600570253	34.98070218203844	24.22143922941689
4	14.076640960865133	26.214986925497495	32.71438121408205	26.99399089955532
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.46736175744951	0.3215995213402473	0.15456721180694058	0.05647150940330038
12	99.27828007504912	0.45893719806763283	0.14241604987516435	0.12036667700808107
13	96.65163468214925	2.151457400759355	0.5607077959491202	0.6362001211422831
14	58.49438239595799	28.176605504587155	5.556811298733915	7.7722008007209435
15	42.63826101729971	34.86293932544431	13.572829410982582	8.925970246273398
16	45.67970792891016	31.181229594173352	14.722148354976436	8.41691412194005
17	30.651361373339835	33.49669074738879	19.228105748349066	16.623842130922306
18	24.027408441548847	44.358943107447296	17.905752781101803	13.70789566990205
19	26.766590583403506	44.10740666873495	18.090956433098434	11.035046314763107
20	19.010178906469292	48.04785489518238	20.515242506167915	12.426723692180413
21	20.421338769962624	49.59203120152462	16.180010784617885	13.806619243894872
22	22.936186086365584	49.67788340793925	17.099159390742958	10.286771114952208
23	17.12748747950184	46.768344930490926	21.128488380682235	14.975679209325001
24	18.685864763846418	49.87710336982376	16.961969448507144	14.47506241782269
25	18.415178241664083	43.48498278893173	22.978936754864158	15.120902214540028
26	19.53238340055253	43.99056715271315	22.16120787720309	14.315841569531237
27	24.566528534917047	45.962693347515845	18.467346984000354	11.003431133566755
28	27.301777245933607	44.14555910118335	17.829189380844745	10.723474272038294
29	31.39202824683479	41.67342551965608	16.97269866595754	9.961847567551596
30	35.43027670670271	42.80904208955665	12.90623291819941	8.854448285541224
31	31.97572722303476	45.64833281626261	11.911758188184194	10.46418177251843
32	30.022695711267712	50.50043212338785	12.517820473045843	6.959051692298601
33	28.529598605386397	53.080965888105894	11.719315546100548	6.670119960407156
34	25.156690895123283	55.250797766254486	12.880120846814105	6.712390491808122
35	25.584936252566887	55.47814268197197	12.005717324823827	6.931203740637326
36	20.24293090457829	57.622139490906946	13.510947125825467	8.6239824786893
37	18.920504070085244	59.366828435934934	13.982367888430913	7.7302996055489075
38	18.11786257737594	60.588389546307376	13.415843046876155	7.87790482944053
39	18.989514544460697	61.10549720043138	11.671320303151177	8.233667951956743
40	19.21772740031615	61.62424840077413	11.278106487021525	7.8799177118881945
41	19.812229461212308	61.4554986777763	11.218237084312074	7.514034776699316
42	21.301300802198288	58.49589667449659	11.87665277962446	8.326149743680658
43	22.748249346274875	57.40766594276766	10.221897206340765	9.622187504616702
44	21.026496181063393	56.523696612448106	11.394927536231885	11.054879670256614
45	21.096836265862994	55.73778605090931	13.708191138885194	9.457186544342507
46	22.37189572899585	55.36368538462675	12.667937183294184	9.596481703083219
47	25.13056035692653	54.697328960392376	10.318718698754598	9.853391983926487
48	25.09338666548479	54.432237882078326	10.28032619775739	10.19404925467949
49	24.251059994977027	54.934738288348186	10.314637533424929	10.499564183249865
50	23.009517794619512	55.391976539762744	10.694407510821552	10.9040981547962
51	21.829894813042	55.48887189942236	11.223832528180354	11.457400759355286
52	21.53106487021525	55.17768765973792	11.500428430025558	11.790819040021274
53	20.675571363146155	55.04382174356247	11.76657211659206	12.514034776699315
54	20.275192424175273	54.26919440381746	12.36875637104995	13.08685680095732
55	20.47186396608016	52.96896467668306	12.844683035648332	13.714488321588442
56	20.544937138973836	51.73621267857407	13.450782254132873	14.268067928319224
57	20.832022189720632	49.732896039238284	14.143084548449528	15.291997222591558
58	21.270738229254384	47.605777895965375	15.201509846503864	15.92197402827638
59	21.538211526245032	45.86795860479546	16.280544106132457	16.31328576282705
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	7.0
5	12.0
6	24.5
7	37.0
8	322.0
9	1389.0
10	2171.0
11	3705.5
12	5240.0
13	7236.0
14	10337.5
15	11443.0
16	12217.5
17	12992.0
18	14410.0
19	18635.0
20	21442.0
21	29091.0
22	36740.0
23	52074.5
24	67409.0
25	93857.5
26	156787.5
27	193269.0
28	232563.0
29	271857.0
30	307986.5
31	367291.5
32	390467.0
33	398824.5
34	407182.0
35	403324.0
36	387412.0
37	375358.0
38	363787.0
39	352216.0
40	338865.0
41	325514.0
42	315800.5
43	296815.5
44	287544.0
45	279360.5
46	271177.0
47	262007.5
48	242912.5
49	232987.0
50	217862.5
51	202738.0
52	183432.0
53	144314.5
54	124503.0
55	103225.0
56	81947.0
57	67192.0
58	40917.5
59	29398.0
60	24059.5
61	18721.0
62	15064.0
63	11407.0
64	9230.5
65	5197.0
66	3340.0
67	2938.5
68	2537.0
69	1953.0
70	1070.0
71	771.0
72	663.5
73	556.0
74	421.0
75	222.0
76	158.0
77	127.5
78	97.0
79	93.0
80	89.0
81	56.5
82	17.0
83	10.0
84	7.5
85	5.0
86	5.0
87	3.5
88	2.0
89	1.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	5415120.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.01727725538514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.48072210027817	58.17792917805854
2	4.24355507981587	5.518087943457729
3	1.62133698232181	3.162447483120798
4	0.9069682576805228	2.3587442668579253
5	0.639147019256167	2.0777799478965595
6	0.4727789213067237	1.84432789242607
7	0.3721680584101711	1.6938147687476721
8	0.30461987679839436	1.5844443989841976
9	0.23551684932394115	1.3781397861728504
>10	1.6770140683603676	18.94476043420322
>50	0.03551206061956078	1.4938514015035445
>100	0.009519060161539361	1.1689176733725672
>500	9.133338741340761E-4	0.406398254358324
>1k	2.283317927029164E-4	0.19035657083998442
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.012631299029384391	0.0	0.0	0.0	0.0
12	0.019556353321810042	0.0	0.0	0.0	0.0
13	0.033369528283768414	0.0	0.0	0.0	0.0
14	0.16031039016679224	0.0	0.0	0.0	0.0
15	0.33648377136610086	0.0	0.0	0.0	0.0
16	0.4671549291613113	0.0	0.0	0.0	0.0
17	0.5816491601294154	0.0	0.0	0.0	0.0
18	0.7203903145267325	0.0	0.0	0.0	0.0
19	0.8793895610808256	0.0	0.0	0.0	0.0
20	1.048305485381672	0.0	0.0	0.0	0.0
21	1.2035375024006856	0.0	0.0	0.0	0.0
22	1.3586771853624666	0.0	0.0	0.0	0.0
23	1.5090708977825054	0.0	0.0	0.0	0.0
24	1.6862045531770302	0.0	0.0	0.0	0.0
25	1.8858677185362467	1.8466811446468408E-5	0.0	0.0	0.0
26	2.1104241457253026	1.8466811446468408E-5	0.0	0.0	0.0
27	2.3616466486430587	1.8466811446468408E-5	0.0	0.0	0.0
28	2.626793127391452	1.8466811446468408E-5	0.0	0.0	0.0
29	2.9096492783170085	1.8466811446468408E-5	0.0	0.0	0.0
30	3.2217753253852175	1.8466811446468408E-5	0.0	0.0	0.0
31	3.534104507379338	1.8466811446468408E-5	0.0	0.0	0.0
32	3.8296288909571716	1.8466811446468408E-5	0.0	0.0	0.0
33	4.119022293134778	1.8466811446468408E-5	0.0	0.0	0.0
34	4.398702152491542	1.8466811446468408E-5	0.0	0.0	0.0
35	4.663977898920061	1.8466811446468408E-5	0.0	0.0	0.0
36	4.921811520335653	1.8466811446468408E-5	0.0	0.0	0.0
37	5.17946047363678	1.8466811446468408E-5	0.0	0.0	0.0
38	5.4330282616082375	1.8466811446468408E-5	0.0	0.0	0.0
39	5.689901608828613	1.8466811446468408E-5	0.0	0.0	0.0
40	5.948898639365333	1.8466811446468408E-5	0.0	0.0	0.0
41	6.221302575012188	1.8466811446468408E-5	0.0	0.0	0.0
42	6.508461493004772	1.8466811446468408E-5	0.0	0.0	0.0
43	6.820716807753106	1.8466811446468408E-5	0.0	0.0	0.0
44	7.14949991874603	1.8466811446468408E-5	0.0	0.0	1.8466811446468408E-5
45	7.495143228589579	1.8466811446468408E-5	0.0	0.0	1.8466811446468408E-5
46	7.852531430513082	1.8466811446468408E-5	0.0	0.0	1.8466811446468408E-5
47	8.215311941378953	1.8466811446468408E-5	0.0	0.0	1.8466811446468408E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTTA	20	3.1392864E-4	53.0	22
ACTAGGT	15	0.006697603	53.0	48
TAACCGT	20	3.1392864E-4	53.0	16
CTACGCG	15	0.006697603	53.0	26
TACCTAA	40	1.6025297E-9	53.0	21
CCGTCAA	115	0.0	50.695652	17
TCTAGGA	120	0.0	48.583332	32
TCAGCTA	140	0.0	47.321434	44
TGGGCGC	4755	0.0	46.590954	10
TACTGGC	165	0.0	46.57576	27
TAACGTC	80	0.0	46.375	36
ACGGATT	80	0.0	46.375	31
CTATCTA	70	0.0	45.428577	24
CGATTAA	35	2.0301122E-6	45.428577	34
TACTCTA	70	0.0	45.428577	52
CAGCTAA	70	0.0	45.428577	25
CATTTCG	35	2.0301122E-6	45.428577	29
TGGGCAG	26965	0.0	45.42296	10
CGCGATA	135	0.0	45.148148	32
CTACGGT	135	0.0	45.148148	44
>>END_MODULE