FastQCFastQC Report
Fri 17 Jun 2016
SRR1528032_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528032_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118627
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTTGCGGGGTACCTGGCCTTTCTCGCCGGCCTGTTGATCCTGATGCT18771.582270478053057No Hit
GTCCATTTGCGGGGTGGGGAGTGCCAGTGTGTATGTATGTGTGTGAGGTGCG8010.6752257074696317No Hit
GCCTATTTGCGGGCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCC7520.6339197653148103No Hit
TGTTATTTGCGGGGGCTAAGGCGGGCGGATCACGAGGTCAGGAGATCGAGAC6510.5487789457711988No Hit
ATATATTTGCCGGGGGAACGAACTGTAGCAAGCTTGGAGCCCGCCCCGCGGC5840.4922993922125654No Hit
TCTGATTTGCGGGGGCTGGGCACAGTGGCTTATGCCTGTAATCCCAGCCACT5250.4425636659445151No Hit
GAAAATTTGCCGGGGGCCCCGACGCCTGCGTACAGACAACCGCGCCGGCTTT5110.4307619681859947No Hit
GTTTATTTGCGGGAATACATTAACTGGGCGTGGTGGCGGGCACCTGTAATAC5010.4223321840727659No Hit
AATCATTTGCGGGGCAACAGCCATTCCACGACTCTTTGCCGTGCCAAAGTCA4670.39367091808778776No Hit
GCGAATTTGCGGGAGCAGTGGTATCAACCCAGAGTGTTTATTTGCGGGGACC4160.35067901911032057No Hit
TATTATTTGCGGGTGACTCCATTGAAGTCTGGCGTGAAGATGAGCTTGTCGG3900.32876158041592557No Hit
GGGCATTTGCGGGGATTGGACTTCATAAAAATTAAAAACTGTGTGTGTCAAA3820.3220177531253425No Hit
CGCAATTTGCGGGGGAAGCCCTCGATGCCAATTCTCGGAAGCAAGAAGCGGA3820.3220177531253425No Hit
GGGGATTTGCTCCGACTTTTCTGTGTTGGCTTCACTAAGAAACGCAACAACC3580.3017862712535932No Hit
TTAAATTTGCGGGAATTGATCACGCTTTCTCATTAAAAATAGAACCAGTAGG3580.3017862712535932No Hit
ACGTATTTGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC3580.3017862712535932No Hit
CGGAATTTGCGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCATC3510.29588542237433296No Hit
TTGCATTTGCGGCCTCTGAAAGCACGTATTTTAAACTGGTAAGAATTCTGCT3510.29588542237433296No Hit
CTAGATTTGCGGGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCG3170.26722415638935487No Hit
GGCAATTTGCGGGCACAAGTATGACTCAGTATGATCACTTTTAAAAACATTG3130.2638522427440633No Hit
GCGGATTTGCGGGGGCTGAGTAAGGGCACACGGAGGATGCCTAGGCGGCGGG3120.26300926433274047No Hit
GCGGATTTGCGGGTGGGCTTCCTCAGGGACATTTTCTACTGACTGCTTTTTT2890.24362076087231405No Hit
GCGGATTTGCGGGGAAACAAAAATTACTTTTTAAGTGAAATTTTTTATTTAT2680.22591821423453345No Hit
GAGGATTTGCGGGATGATCATCAGGTTGGGCAGAAAGACAAACAACACAAGC2550.21495949488733596No Hit
TCTCATTTGCGGGGCCAAATCCGTAAATTTGTGCTACCATATATTGCGTTCG2520.21243055965336727No Hit
ACAAATTTGCGGGTGAGACTGAAAAAACCGGCACCATGGTGCGTTTCTGGCC2510.2115875812420444No Hit
CATTATTTGCCTTTTTATCTCCCATCTGGCCACACTGCTGCCGCCACCTCTC2500.2107446028307215No Hit
AGCAATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT2480.20905864600807575No Hit
GTCCATTTGCGGGGGTATCAACGCAGAGTACAAATTTGCGGGCCCAAATCAG2330.19641396983823245No Hit
TCAAATTTGCGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTGG2300.1938850346042638No Hit
TAGGATTTGCGGGTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTC2280.19219907778161802No Hit
GGACATTTGCGGGTCTCTGCTAAAAATACAAAAATTAGCTGTGCGTGGTGGT2270.19135609937029513No Hit
CTTTATTTGCGGGCTTCAGCCCAGCCCCATTCACACCTCAAGGCAGTTGCAG2260.19051312095897224No Hit
CTTTATTTGCGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT2240.18882716413632647No Hit
AATGATTTGCGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG2140.1803973800230976No Hit
GCTAATTTGCGGGGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATCTC2110.17786844478912897No Hit
GGAGATTTGCGGGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCC2110.17786844478912897No Hit
TGGTATTTGCGGGGGGAATTCAGATGTATATATGATGTCCTTGGACGGGGTG2100.17702546637780606No Hit
CTTCATTTGCGGGAAACTGAAATGGGAAGGAAAATCAGCCAAACATAAACAA2060.1736535527325145No Hit
CTTTATTTGCGGGGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGAC2000.1685956822645772No Hit
TTAGATTTGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC1980.16690972544193144No Hit
GCTGATTTGCGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCC1930.16269483338531698No Hit
GCCGATTTGCGGGCAGAGTGAAGATTTGCGGGGCCAAATCAGTAAATTTGTG1920.1618518549739941No Hit
ATAAATTTGCGGAGCTACGCGGGAGGCTGAGGCAGGAGAATGGTGTGAACCC1910.16100887656267124No Hit
AGCTATTTGCGGATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGC1900.16016589815134835No Hit
CAGAATTTGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1890.15932291974002546No Hit
GAAAATTTGCGGGAAAGTGATATTGATGCAGAAAAAAAGATTTAAAGATTAA1880.15847994132870258No Hit
ACTTATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT1870.1576369629173797No Hit
CAGTATTTGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1850.1559510060947339No Hit
AGTTATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT1830.15426504927208814No Hit
GATGATTTGCGGGTAATGCTCCAAAATAATGTTTGACTCCATGTCCTACATC1790.1508931356267966No Hit
CTTAATTTGCGGTACACTGCTTTTGATCTAGAAGGAAGAATCTGGTACAGGG1770.14920717880415083No Hit
GCTGATTTGCGGGAAACATTTCATCCGTAAACCCTTCACAGAACAACAAATG1720.1449922867475364No Hit
GGGTATTTGCGGGGGATCGTGGCGAAGCAAGATCCGGCTATATGGCAGGAGT1690.14246335151356773No Hit
GGTCATTTGCGGGATCAACGCAGAGTCTAAATTTGCGGGCAGTAAATTTGTG1650.13909143786827619No Hit
TAATATTTGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1640.13824845945695333No Hit
TTTGATTTGCGGGACATTTGCGGCCCAAATCAGTAAATTTGTGCTACCATGT1630.13740548104563044No Hit
AGAGATTTGCGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACAT1620.13656250263430753No Hit
GTTAATTTGCGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGA1540.12981867534372443No Hit
CCTAATTTGCGGTAGGGAGACCGGACAGTTTGCACTTCTTGCAGAAAACCAG1530.12897569693240155No Hit
AAAAATTTGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1520.12813271852107866No Hit
TGCTATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT1500.1264467616984329No Hit
TTTGATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT1480.12476080487578713No Hit
AAATATTTGCGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG1450.12223186964181847No Hit
CCTCATTTGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1450.12223186964181847No Hit
CTTAATTTGCGGGGGGTTGTCCCGGGGCGATACCGACCGCCGGCCGGACTCG1400.11801697758520403No Hit
TAATATTTGCGGGGAAATGGATAAACAAAATGAATACATGCATATATGGAAG1380.11633102076255826No Hit
CGGAATTTGCGAGGTTGCCATTGCTTTTGGTGTTTTAGACATGAAGTCCTTG1350.11380208552858961No Hit
GTGCATTTGCGGGGTATTTCAATCAACAATACCAGCGCTGCTTACACAGAAT1350.11380208552858961No Hit
TTTCATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT1330.11211612870594384No Hit
CCTTATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT1300.1095871934719752No Hit
AACAATTTGCGGGAAGCTGGTTATTTGAAAAGATAAATAAAATTGATAGACC1270.10705825823800652No Hit
GCTCATTTGCGGGGTGCTGATTTGCGGGCCAAATCAGTAAATTTGTGCTACC1270.10705825823800652No Hit
TTTTATTTGCGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC1250.10537230141536075No Hit
ATTGATTTGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1220.1028433661813921No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCCG405.5388227E-946.00000417
TAGAAGA206.288472E-446.00000439
TGGTCTT206.288472E-446.00000430
CTTTTAA206.288472E-446.00000438
ACAGCCA800.046.00000417
TTTGAGA206.288472E-446.00000421
ATCCCAG405.5388227E-946.00000441
ACAGTAC206.288472E-446.00000421
TTCACTA405.5388227E-946.00000431
TGTTGGC405.5388227E-946.00000424
AAGTATG206.288472E-446.00000417
CTTCACT405.5388227E-946.00000430
GTAGAAG206.288472E-446.00000438
TTGTCCC206.288472E-446.00000417
ACTGTAG405.5388227E-946.00000422
CCAATTA206.288472E-446.00000427
GTATGAT206.288472E-446.00000429
ACTAAGA405.5388227E-946.00000434
GGTGACT405.5388227E-946.00000412
TGCATCG206.288472E-446.00000425
Produced by FastQC (version 0.11.5)