FastQCFastQC Report
Fri 17 Jun 2016
SRR1528031_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528031_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117529
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTTGCGGGGTACCTGGCCTTTCTCGCCGGCCTGTTGATCCTGATGCT18391.5647201967174058No Hit
GTCCATTTGCGGGGTGGGGAGTGCCAGTGTGTATGTATGTGTGTGAGGTGCG7830.6662185503152414No Hit
GCCTATTTGCGGGCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCC7560.6432454968560951No Hit
TGTTATTTGCGGGGGCTAAGGCGGGCGGATCACGAGGTCAGGAGATCGAGAC6080.5173191297467008No Hit
TCTGATTTGCGGGGGCTGGGCACAGTGGCTTATGCCTGTAATCCCAGCCACT5310.45180338469654296No Hit
ATATATTTGCCGGGGGAACGAACTGTAGCAAGCTTGGAGCCCGCCCCGCGGC5280.4492508232010823No Hit
GTTTATTTGCGGGAATACATTAACTGGGCGTGGTGGCGGGCACCTGTAATAC5000.42542691591011583No Hit
GAAAATTTGCCGGGGGCCCCGACGCCTGCGTACAGACAACCGCGCCGGCTTT4580.3896910549736661No Hit
AATCATTTGCGGGGCAACAGCCATTCCACGACTCTTTGCCGTGCCAAAGTCA4260.3624637323554186No Hit
GCGAATTTGCGGGAGCAGTGGTATCAACCCAGAGTGTTTATTTGCGGGGACC3820.3250261637553285No Hit
CGCAATTTGCGGGGGAAGCCCTCGATGCCAATTCTCGGAAGCAAGAAGCGGA3670.312263356278025No Hit
TTGCATTTGCGGCCTCTGAAAGCACGTATTTTAAACTGGTAAGAATTCTGCT3670.312263356278025No Hit
GGGCATTTGCGGGGATTGGACTTCATAAAAATTAAAAACTGTGTGTGTCAAA3470.29524627964162037No Hit
GGGGATTTGCTCCGACTTTTCTGTGTTGGCTTCACTAAGAAACGCAACAACC3440.2926937181461597No Hit
TATTATTTGCGGGTGACTCCATTGAAGTCTGGCGTGAAGATGAGCTTGTCGG3430.2918428643143394No Hit
CGGAATTTGCGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCATC3310.28163261833249664No Hit
CTAGATTTGCGGGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCG3210.27312408001429433No Hit
GCGGATTTGCGGGTGGGCTTCCTCAGGGACATTTTCTACTGACTGCTTTTTT3050.25951041870517066No Hit
GCGGATTTGCGGGGGCTGAGTAAGGGCACACGGAGGATGCCTAGGCGGCGGG2970.2527035880506088No Hit
GGCAATTTGCGGGCACAAGTATGACTCAGTATGATCACTTTTAAAAACATTG2960.25185273421878857No Hit
ACGTATTTGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC2900.24674761122786718No Hit
TTAAATTTGCGGGAATTGATCACGCTTTCTCATTAAAAATAGAACCAGTAGG2890.24589675739604694No Hit
TCTCATTTGCGGGGCCAAATCCGTAAATTTGTGCTACCATATATTGCGTTCG2700.22973053459146253No Hit
CATTATTTGCCTTTTTATCTCCCATCTGGCCACACTGCTGCCGCCACCTCTC2440.20760833496413653No Hit
GCGGATTTGCGGGGAAACAAAAATTACTTTTTAAGTGAAATTTTTTATTTAT2400.20420491963685558No Hit
TCAAATTTGCGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTGG2360.20080150430957466No Hit
AGCAATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT2340.1990997966459342No Hit
GAGGATTTGCGGGATGATCATCAGGTTGGGCAGAAAGACAAACAACACAAGC2330.19824894281411398No Hit
ACAAATTTGCGGGTGAGACTGAAAAAACCGGCACCATGGTGCGTTTCTGGCC2330.19824894281411398No Hit
GTCCATTTGCGGGGGTATCAACGCAGAGTACAAATTTGCGGGCCCAAATCAG2310.19654723515047348No Hit
GGTCATTTGCGGGATCAACGCAGAGTCTAAATTTGCGGGCAGTAAATTTGTG2170.18463528150499026No Hit
CTTTATTTGCGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT2100.17867930468224863No Hit
TAGGATTTGCGGGTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTC2080.17697759701860818No Hit
CAGAATTTGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG2040.17357418169132727No Hit
TGGTATTTGCGGGGGGAATTCAGATGTATATATGATGTCCTTGGACGGGGTG2040.17357418169132727No Hit
CTTTATTTGCGGGCTTCAGCCCAGCCCCATTCACACCTCAAGGCAGTTGCAG2020.1718724740276868No Hit
GCTGATTTGCGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCC1950.16591649720494517No Hit
GGACATTTGCGGGTCTCTGCTAAAAATACAAAAATTAGCTGTGCGTGGTGGT1940.16506564337312493No Hit
AATGATTTGCGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG1920.16336393570948446No Hit
GCTAATTTGCGGGGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATCTC1890.16081137421402378No Hit
CTTTATTTGCGGGGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGAC1880.15996052038220354No Hit
TTAGATTTGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC1820.15485539739128215No Hit
GGAGATTTGCGGGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCC1810.15400454355946191No Hit
ATAAATTTGCGGAGCTACGCGGGAGGCTGAGGCAGGAGAATGGTGTGAACCC1800.15315368972764168No Hit
GCTGATTTGCGGGAAACATTTCATCCGTAAACCCTTCACAGAACAACAAATG1770.15060112823218097No Hit
CTTAATTTGCGGTACACTGCTTTTGATCTAGAAGGAAGAATCTGGTACAGGG1760.14975027440036076No Hit
CTTCATTTGCGGGAAACTGAAATGGGAAGGAAAATCAGCCAAACATAAACAA1760.14975027440036076No Hit
GATGATTTGCGGGTAATGCTCCAAAATAATGTTTGACTCCATGTCCTACATC1750.14889942056854052No Hit
ACTTATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT1720.14634685907307984No Hit
AGCTATTTGCGGATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGC1700.14464515140943937No Hit
TTTGATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT1690.14379429757761913No Hit
AGTTATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT1670.14209258991397866No Hit
GCCGATTTGCGGGCAGAGTGAAGATTTGCGGGGCCAAATCAGTAAATTTGTG1660.14124173608215845No Hit
GAAAATTTGCGGGAAAGTGATATTGATGCAGAAAAAAAGATTTAAAGATTAA1650.14039088225033822No Hit
CAGTATTTGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1570.13358405159577635No Hit
AGAGATTTGCGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACAT1550.1318823439321359No Hit
CCTAATTTGCGGTAGGGAGACCGGACAGTTTGCACTTCTTGCAGAAAACCAG1520.1293297824366752No Hit
TTTCATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT1490.12677722094121452No Hit
TTTGATTTGCGGGACATTTGCGGCCCAAATCAGTAAATTTGTGCTACCATGT1470.12507551327757405No Hit
GGGTATTTGCGGGGGATCGTGGCGAAGCAAGATCCGGCTATATGGCAGGAGT1440.12252295178211334No Hit
TGCTATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT1410.11997039028665267No Hit
CGGAATTTGCGAGGTTGCCATTGCTTTTGGTGTTTTAGACATGAAGTCCTTG1390.1182686826230122No Hit
TAATATTTGCGGGGAAATGGATAAACAAAATGAATACATGCATATATGGAAG1390.1182686826230122No Hit
TTGTATTTGCGGGGGGGAATCCAGATAAAACGAATAGCTCGTAACCAAACAT1370.11656697495937172No Hit
CCTCATTTGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1340.11401441346391103No Hit
TAATATTTGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1300.1106109981366301No Hit
GTGCATTTGCGGGGTATTTCAATCAACAATACCAGCGCTGCTTACACAGAAT1300.1106109981366301No Hit
GTTAATTTGCGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGA1280.10890929047298965No Hit
CTATATTTGCGGGCCAAATCAGTAAATTTGTGCTTCGCATGATATTCCCTTC1240.10550587514570871No Hit
CTTAATTTGCGGGGGGTTGTCCCGGGGCGATACCGACCGCCGGCCGGACTCG1240.10550587514570871No Hit
TCCTATTTGCGGGTACTTGGTTTCCTTTAGTTTTACCAACCTTACATGGATC1230.10465502131388849No Hit
AAAAATTTGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1200.10210245981842779No Hit
AACAATTTGCGGGAAGCTGGTTATTTGAAAAGATAAATAAAATTGATAGACC1200.10210245981842779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA551.8189894E-1246.00000442
TAGAAGG206.288233E-446.00000429
ACAGTAC206.288233E-446.00000421
AAGTATG405.5370037E-946.00000417
GAGGCAT206.288233E-446.00000445
ACTGCGG206.288233E-446.00000425
ACTGTGT405.5370037E-946.00000438
GTAGAAG206.288233E-446.00000438
ACAAACA206.288233E-446.00000438
GGATGCC206.288233E-446.00000435
GTATGAT405.5370037E-946.00000429
GAGAACG206.288233E-446.00000437
CCAACGC206.288233E-446.00000425
TTCATAA405.5370037E-946.00000422
CCGGAGT800.046.00000423
AATCTGG206.288233E-446.00000439
GGGTCAA405.5370037E-946.00000411
TAGGAGG206.288233E-446.00000442
CACACGG206.288233E-446.00000427
GAAACTG206.288233E-446.00000413
Produced by FastQC (version 0.11.5)