FastQCFastQC Report
Fri 17 Jun 2016
SRR1528030_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528030_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120745
Sequences flagged as poor quality0
Sequence length59
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTTGCGGGGTACCTGGCCTTTCTCGCCGGCCTGTTGATCCTGATGCTGCCCCTG17801.4741811255124435No Hit
GTCCATTTGCGGGGTGGGGAGTGCCAGTGTGTATGTATGTGTGTGAGGTGCGAGTGAAT7860.6509586318274049No Hit
GCCTATTTGCGGGCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGG6880.5697958507598658No Hit
ATATATTTGCCGGGGGAACGAACTGTAGCAAGCTTGGAGCCCGCCCCGCGGCTCGCGTG5790.4795229616133173No Hit
TCTGATTTGCGGGGGCTGGGCACAGTGGCTTATGCCTGTAATCCCAGCCACTCAGGAGG5560.46047455381175206No Hit
GTTTATTTGCGGGAATACATTAACTGGGCGTGGTGGCGGGCACCTGTAATACCAGCTAC4890.4049857136941488No Hit
GAAAATTTGCCGGGGGCCCCGACGCCTGCGTACAGACAACCGCGCCGGCTTTGGCTCCC4850.4016729471199636No Hit
AATCATTTGCGGGGCAACAGCCATTCCACGACTCTTTGCCGTGCCAAAGTCAGGGCAGC4840.40084475547641724No Hit
GCGAATTTGCGGGAGCAGTGGTATCAACCCAGAGTGTTTATTTGCGGGGACCGTAGCGC4290.3552942150813698No Hit
GGGCATTTGCGGGGATTGGACTTCATAAAAATTAAAAACTGTGTGTGTCAAAGGATACT3900.32299474098306347No Hit
TATTATTTGCGGGTGACTCCATTGAAGTCTGGCGTGAAGATGAGCTTGTCGGCGGTATG3890.32216654933951716No Hit
TGTTATTTGCGGGGGCTAAGGCGGGCGGATCACGAGGTCAGGAGATCGAGACCATCCCG3840.3180255911217856No Hit
GGGGATTTGCTCCGACTTTTCTGTGTTGGCTTCACTAAGAAACGCAACAACCAGATAAG3760.311400057973415No Hit
TTGCATTTGCGGCCTCTGAAAGCACGTATTTTAAACTGGTAAGAATTCTGCTTATACCT3680.3047745248250445No Hit
ACGTATTTGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGT3550.29400803345894244No Hit
CGCAATTTGCGGGGGAAGCCCTCGATGCCAATTCTCGGAAGCAAGAAGCGGAGTGGAAA3450.2857261170234792No Hit
GCGGATTTGCGGGGGCTGAGTAAGGGCACACGGAGGATGCCTAGGCGGCGGGAGCCGAA3390.28075696716220133No Hit
CGGAATTTGCGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCATCCTTGGCG3220.2666777092219139No Hit
GCGGATTTGCGGGTGGGCTTCCTCAGGGACATTTTCTACTGACTGCTTTTTTTCTACAT3210.26584951757836767No Hit
GGCAATTTGCGGGCACAAGTATGACTCAGTATGATCACTTTTAAAAACATTGTATATTG3200.2650213259348213No Hit
CTAGATTTGCGGGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGC3040.25177025963808025No Hit
TTAAATTTGCGGGAATTGATCACGCTTTCTCATTAAAAATAGAACCAGTAGGCCAGACG2880.23851919334133917No Hit
CATTATTTGCCTTTTTATCTCCCATCTGGCCACACTGCTGCCGCCACCTCTCACACAAC2880.23851919334133917No Hit
GCGGATTTGCGGGGAAACAAAAATTACTTTTTAAGTGAAATTTTTTATTTATCTAGAAT2710.2244399354010518No Hit
AGCAATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA2600.21532982732204234No Hit
GAGGATTTGCGGGATGATCATCAGGTTGGGCAGAAAGACAAACAACACAAGCCAACCGA2420.20042237773820862No Hit
ACAAATTTGCGGGTGAGACTGAAAAAACCGGCACCATGGTGCGTTTCTGGCCCAGCCTC2400.198765994451116No Hit
CTTTATTTGCGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGAC2380.19710961116402334No Hit
TCTCATTTGCGGGGCCAAATCCGTAAATTTGTGCTACCATATATTGCGTTCGCATGATA2360.19545322787693073No Hit
GAAAATTTGCGGGAAAGTGATATTGATGCAGAAAAAAAGATTTAAAGATTAAAAAAAAA2330.19296865294629176No Hit
CTTTATTTGCGGGCTTCAGCCCAGCCCCATTCACACCTCAAGGCAGTTGCAGGGCCCAC2290.1896558863721065No Hit
GGACATTTGCGGGTCTCTGCTAAAAATACAAAAATTAGCTGTGCGTGGTGGTGCGCACC2200.18220216158018965No Hit
TGGTATTTGCGGGGGGAATTCAGATGTATATATGATGTCCTTGGACGGGGTGGCGCAGT2110.17474843678827282No Hit
TAGGATTTGCGGGTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAA2100.17392024514472648No Hit
CAGAATTTGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCAT2080.17226386185763387No Hit
GGAGATTTGCGGGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGA2070.17143567021408754No Hit
GCTAATTTGCGGGGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAA2060.17060747857054123No Hit
CTTTATTTGCGGGGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCAATCCC1960.16232556213507807No Hit
AATGATTTGCGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGCG1950.16149737049153173No Hit
CTTCATTTGCGGGAAACTGAAATGGGAAGGAAAATCAGCCAAACATAAACAAAAAAGAA1850.1532154540560686No Hit
CTTAATTTGCGGTACACTGCTTTTGATCTAGAAGGAAGAATCTGGTACAGGGAAAAAAA1800.14907449583833698No Hit
GCTGATTTGCGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTC1800.14907449583833698No Hit
GCTGATTTGCGGGAAACATTTCATCCGTAAACCCTTCACAGAACAACAAATGCTATGAC1800.14907449583833698No Hit
GATGATTTGCGGGTAATGCTCCAAAATAATGTTTGACTCCATGTCCTACATCCAGGGCA1790.14824630419479068No Hit
CAGTATTTGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCAT1780.14741811255124435No Hit
AGCTATTTGCGGATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTC1750.1449335376206054No Hit
AGTTATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA1710.14162077104642015No Hit
GTCCATTTGCGGGGGTATCAACGCAGAGTACAAATTTGCGGGCCCAAATCAGTAAATTT1630.1349952378980496No Hit
AACAATTTGCGGGAAGCTGGTTATTTGAAAAGATAAATAAAATTGATAGACCACTAGCA1580.13085427968031804No Hit
CGGAATTTGCGAGGTTGCCATTGCTTTTGGTGTTTTAGACATGAAGTCCTTGCCCATGC1560.1291978963932254No Hit
TTTCATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA1550.12836970474967907No Hit
AAAAATTTGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCAT1550.12836970474967907No Hit
TTTGATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA1530.12671332146258643No Hit
TTAGATTTGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGT1520.12588512981904013No Hit
ATAAATTTGCGGAGCTACGCGGGAGGCTGAGGCAGGAGAATGGTGTGAACCCGGGAGGC1500.12422874653194749No Hit
TGCTATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA1490.12340055488840118No Hit
GCCGATTTGCGGGCAGAGTGAAGATTTGCGGGGCCAAATCAGTAAATTTGTGCTACCAT1470.12174417160130856No Hit
GTTAATTTGCGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATT1470.12174417160130856No Hit
ACTTATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA1460.12091597995776222No Hit
TTGTATTTGCGGGGGGGAATCCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGC1440.11925959667066958No Hit
CCTAATTTGCGGTAGGGAGACCGGACAGTTTGCACTTCTTGCAGAAAACCAGAGAGAAC1390.11511863845293802No Hit
CCTTATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA1380.11429044680939168No Hit
AGAGATTTGCGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGC1370.11346225516584538No Hit
TATTATTTGCGGGACCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAA1360.11263406352229904No Hit
TAATATTTGCGGGGAAATGGATAAACAAAATGAATACATGCATATATGGAAGCCAGGCA1350.11180587187875274No Hit
TTTTATTTGCGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCAAGAA1320.10932129694811381No Hit
GTGCATTTGCGGGGTATTTCAATCAACAATACCAGCGCTGCTTACACAGAATCCATCAA1310.10849310530456747No Hit
CCTCATTTGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCAT1310.10849310530456747No Hit
GGTCATTTGCGGGATCAACGCAGAGTCTAAATTTGCGGGCAGTAAATTTGTGCTACCAT1300.10766491366102117No Hit
GGGTATTTGCGGGGGATCGTGGCGAAGCAAGATCCGGCTATATGGCAGGAGTCGATGCT1290.10683672201747484No Hit
TTTGATTTGCGGGACATTTGCGGCCCAAATCAGTAAATTTGTGCTACCATGTATTGCGG1280.10600853037392853No Hit
TAATATTTGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCAT1280.10600853037392853No Hit
AAATATTTGCGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGCG1250.10352395544328957No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCAG550.053.00000441
CCTGTTG1750.053.00000435
CCAGCCA550.053.00000444
TGCGATT550.053.0000041
GCGGCCT550.053.0000049
TGCCTGT550.053.00000433
CTGGCCT1750.053.00000418
GCTGCCC1750.053.00000450
TCTCGCC1750.053.00000426
TTCTCGC1750.053.00000425
GGCTTAT550.053.00000427
TAATCCC550.053.00000439
CGGCCTG1750.053.00000432
GCCTGTT1750.053.00000434
GTAATCC550.053.00000438
TGTAATC550.053.00000437
TGGCCTT1750.053.00000419
TGGCTTA550.053.00000426
CCGGCTT550.053.00000445
GCTTATG550.053.00000428
Produced by FastQC (version 0.11.5)