FastQCFastQC Report
Fri 17 Jun 2016
SRR1528029_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528029_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1611145
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGACACTGGGGATCACACAGGATCTGGAGCTGGTGCTGATAACAGCGGA24590.15262437583209457No Hit
AGCCGACACTGGGATCCACGATGGCCTAGCACGTGGCATACGCGAAGCTGCC22450.13934189660148527No Hit
GATGGACACTGGGGTCCCCTCCCTCGGCTCTAGACTTGGGGGAGGGGTTGCC16510.10247370658755109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA551.8189894E-1246.00000432
GATCGTA551.8189894E-1246.00000431
CAAGTGG551.8189894E-1246.00000436
ACATTCG551.8189894E-1246.00000445
CCTAATA551.8189894E-1246.00000421
CCGCTAA551.8189894E-1246.00000443
TCGGAAT551.8189894E-1246.00000436
TAACCGT551.8189894E-1246.00000426
TGCGCAA551.8189894E-1246.00000436
CGCTAAG551.8189894E-1246.00000444
AATCTCG551.8189894E-1246.00000441
ACCCGTT750.046.00000439
CGTCAGA1150.046.00000435
TCACGGT1000.046.031
CGGACAA253.4177712E-546.032
TCACGCT301.8618721E-646.031
CTTCGTA253.4177712E-546.020
CTTAACG301.8618721E-646.029
GTGTTAA253.4177712E-546.016
ACAACCG405.6134013E-946.036
Produced by FastQC (version 0.11.5)