FastQCFastQC Report
Fri 17 Jun 2016
SRR1528028_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528028_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597364
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGACACTGGGGATCACACAGGATCTGGAGCTGGTGCTGATAACAGCGGA24470.15318988032784012No Hit
AGCCGACACTGGGATCCACGATGGCCTAGCACGTGGCATACGCGAAGCTGCC23000.14398721894320893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTC206.312196E-446.00000427
AACCGTC351.01990736E-746.00000437
TAATAGC206.312196E-446.00000419
TAATACG351.01990736E-746.00000446
ACTATCT750.046.00000430
GCCCACG206.312196E-446.00000424
ACGCCGT351.01990736E-746.00000430
ATAATTC206.312196E-446.00000423
TAAACTC206.312196E-446.00000422
TAAACGG351.01990736E-746.00000425
ATTGTAC405.6134013E-946.00000430
CGATTTC405.6134013E-946.00000415
GTCTAGT206.312196E-446.00000423
TAAGTCG206.312196E-446.00000444
ATATGGT206.312196E-446.00000443
CGATTAG351.01990736E-746.00000439
CCTAATA351.01990736E-746.00000421
TAACGAA206.312196E-446.00000426
ACTTAGC405.6134013E-946.00000426
CATTCCG405.6134013E-946.00000422
Produced by FastQC (version 0.11.5)