FastQCFastQC Report
Fri 17 Jun 2016
SRR1528027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632086
Sequences flagged as poor quality0
Sequence length59
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGACACTGGGGATCACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCT25760.15783481997884916No Hit
AGCCGACACTGGGATCCACGATGGCCTAGCACGTGGCATACGCGAAGCTGCCAAAGCCT22340.13688004186053923No Hit
GATGGACACTGGGGTCCCCTCCCTCGGCTCTAGACTTGGGGGAGGGGTTGCCACAGGCC16810.10299702344116671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATG503.6379788E-1253.00000449
TCGTTAG251.4790081E-553.00000444
ACACGTA800.053.00000429
CTCCGTA251.4790081E-553.00000433
TAGACCG203.138635E-453.00000431
ACACCTA401.6025297E-953.00000429
CTATACG251.4790081E-553.00000426
GGCGTAA251.4790081E-553.00000448
GCTATAT503.6379788E-1253.00000421
GCTATAA203.138635E-453.00000447
ATTCGGC251.4790081E-553.00000446
ATTCGCG1600.053.00000447
CGTCTAG203.138635E-453.00000443
CAATTCG503.6379788E-1253.00000450
CCTAATA800.053.00000421
TAACGGC1550.053.00000440
CCTAACC203.138635E-453.00000441
GCCATAC203.138635E-453.00000421
ATGTACG251.4790081E-553.00000445
TAGTTAG251.4790081E-553.00000442