##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528027_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1632086
Sequences flagged as poor quality	0
Sequence length	59
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.443053858681466	31.0	31.0	34.0	30.0	34.0
2	31.97137650834576	33.0	31.0	34.0	30.0	34.0
3	32.21747077053538	34.0	31.0	34.0	30.0	34.0
4	35.84081292284843	37.0	35.0	37.0	35.0	37.0
5	35.539214845296144	37.0	35.0	37.0	33.0	37.0
6	35.63008444407954	37.0	35.0	37.0	33.0	37.0
7	35.800546049656695	37.0	35.0	37.0	33.0	37.0
8	35.84908883477954	37.0	35.0	37.0	35.0	37.0
9	37.57677597871681	39.0	37.0	39.0	35.0	39.0
10	37.672889173732266	39.0	37.0	39.0	35.0	39.0
11	37.79513273197613	39.0	37.0	39.0	35.0	39.0
12	37.87081808189029	39.0	37.0	39.0	35.0	39.0
13	37.91657241101265	39.0	38.0	39.0	35.0	39.0
14	38.220543525279915	40.0	38.0	40.0	34.0	40.0
15	37.57724776758087	40.0	37.0	40.0	32.0	40.0
16	37.08737652305087	39.0	36.0	40.0	32.0	40.0
17	37.021552173108525	39.0	36.0	40.0	32.0	40.0
18	37.003620519997106	39.0	36.0	40.0	32.0	40.0
19	37.15240067006273	39.0	36.0	40.0	32.0	40.0
20	37.2741938843909	40.0	36.0	40.0	32.0	40.0
21	37.24793791503634	40.0	36.0	40.0	32.0	40.0
22	37.23578965814301	40.0	36.0	40.0	32.0	40.0
23	37.14385884077187	40.0	36.0	40.0	32.0	40.0
24	37.12066766089532	40.0	36.0	40.0	32.0	40.0
25	37.03506187786673	39.0	36.0	40.0	31.0	40.0
26	36.864141963107336	39.0	36.0	40.0	31.0	40.0
27	36.73224817809846	39.0	35.0	40.0	31.0	40.0
28	36.70580962032638	39.0	35.0	40.0	31.0	40.0
29	36.67022632385793	39.0	35.0	40.0	31.0	40.0
30	36.65825575367965	39.0	35.0	40.0	30.0	40.0
31	36.624484861704595	39.0	35.0	40.0	31.0	40.0
32	36.532255653194746	39.0	35.0	40.0	30.0	40.0
33	36.36884943563023	39.0	35.0	40.0	30.0	40.0
34	36.31545457776122	39.0	35.0	40.0	30.0	40.0
35	36.18183845704209	39.0	35.0	40.0	30.0	40.0
36	36.009182114177804	38.0	35.0	40.0	30.0	40.0
37	35.85897924496626	38.0	35.0	40.0	29.0	40.0
38	35.630808670621526	38.0	35.0	40.0	28.0	40.0
39	35.46459622838503	38.0	34.0	40.0	28.0	40.0
40	35.28116471803569	38.0	34.0	40.0	27.0	40.0
41	35.088745323469475	37.0	34.0	40.0	27.0	40.0
42	34.95802365806704	37.0	34.0	40.0	27.0	40.0
43	34.68757161080973	37.0	33.0	40.0	26.0	40.0
44	34.5347444926309	37.0	33.0	40.0	26.0	40.0
45	34.320138767197314	36.0	33.0	40.0	25.0	40.0
46	34.12120194646606	36.0	33.0	39.0	24.0	40.0
47	33.94187377380849	36.0	33.0	39.0	24.0	40.0
48	33.720859685090126	35.0	33.0	39.0	23.0	40.0
49	33.55653256017146	35.0	33.0	39.0	23.0	40.0
50	33.29688876689096	35.0	32.0	39.0	23.0	40.0
51	33.078965814301455	35.0	32.0	38.0	22.0	40.0
52	32.85939895324143	35.0	32.0	38.0	22.0	40.0
53	32.55240655210571	35.0	31.0	38.0	20.0	40.0
54	32.333056591380604	35.0	31.0	37.0	20.0	40.0
55	32.11380711555641	35.0	31.0	37.0	20.0	40.0
56	31.838663526309276	35.0	31.0	37.0	19.0	40.0
57	31.644079417383644	35.0	31.0	37.0	18.0	39.0
58	31.40564651617623	34.0	30.0	36.0	18.0	39.0
59	30.876484449961584	34.0	29.0	36.0	17.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	8.0
15	45.0
16	137.0
17	432.0
18	920.0
19	1752.0
20	3075.0
21	4583.0
22	6505.0
23	9136.0
24	12343.0
25	16291.0
26	21026.0
27	26426.0
28	32366.0
29	38390.0
30	46031.0
31	55470.0
32	68099.0
33	84529.0
34	113464.0
35	158431.0
36	192013.0
37	289500.0
38	367021.0
39	84089.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.99715088543128	24.7142613808341	36.89358281365075	13.395004920083869
2	16.60188249883891	23.010797225146224	37.12586224010254	23.261458035912323
3	18.21399117448468	19.631624804085078	38.65978876113146	23.494595260298784
4	23.333574333705453	21.134364243060723	33.62837497533831	21.903686447895517
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	100.0	0.0
11	99.48403454229741	0.30482462321225723	0.1357158875206331	0.07542494696970625
12	99.493776675984	0.3198973583499889	0.09276471950620249	0.09356124615982246
13	98.38507284542604	0.8775272871650145	0.2960015587413899	0.44139830866755797
14	60.53957940941837	24.869155179322657	6.8487199816676325	7.742545429591333
15	33.16694095776816	24.623028443354087	20.95698388442766	21.253046714450097
16	29.9922920728442	17.334809562731376	28.68274098301192	23.990157381412498
17	23.99119899319031	18.125209088246574	30.305020691311608	27.5785712272515
18	22.214576927931493	20.351317271271245	32.13819614897744	25.29590965181982
19	22.8271671958463	21.0420896938029	29.09840535363945	27.03233775671135
20	26.565634408971096	20.28667606976593	24.880919265283815	28.266770255979157
21	29.267881717017364	20.3285243547215	20.31749552413292	30.086098404128215
22	29.892297342174373	20.2279169112412	21.43545131812907	28.444334428455363
23	29.480125434566563	21.583666546983434	20.525388980727733	28.41081903772228
24	29.134371595614443	22.884762200031126	20.35058201589867	27.630284188455757
25	32.05811458464811	20.815263411364352	21.25298544316905	25.873636560818486
26	29.280871228599477	21.252617815482765	21.419091886089337	28.04741906982843
27	28.93934510803965	23.477071673919145	21.604376239977547	25.979206978063658
28	31.02489697234092	22.674479163475453	21.255497565692004	25.04512629849162
29	29.859272121689667	22.99076151624363	19.822362301986537	27.32760406008017
30	29.741753804640197	22.91147647856792	19.69675617583877	27.65001354095311
31	28.423808549304386	23.975942444209437	20.27344147305963	27.326807533426546
32	28.400402919944167	25.241990924497852	19.907529382642828	26.450076772915153
33	29.181305396896978	25.27532250138779	20.267130531111718	25.27624157060351
34	28.98921993081247	24.06766555193783	20.56968811692521	26.37342640032449
35	29.016363108316597	25.75066509975577	19.574458698867584	25.658513093060048
36	31.12538187325913	24.126057082776274	19.39713961151557	25.351421432449023
37	30.035549597263874	24.611386899955026	19.768137218259334	25.58492628452177
38	29.30881093275722	26.740870272767488	21.30414696284387	22.64617183163142
39	30.681287628225473	25.647177906066226	21.346669231890967	22.324865233817334
40	30.152332658940768	26.01321253904512	18.9905433904831	24.84391141153101
41	29.736484474470092	27.75533887307409	19.305722860192418	23.202453792263398
42	31.12507551685389	27.13594749296299	18.59025811139854	23.148718878784575
43	28.99424417585838	26.809432836259855	19.439110439033236	24.75721254884853
44	28.71723671424177	27.42919184405724	20.08435829974646	23.76921314195453
45	25.947284640637807	27.29127019041889	20.09832815182533	26.663117017117973
46	25.88129547094945	24.851937949348258	22.32633574456248	26.940430835139818
47	27.64070030623386	24.53142787818779	22.57540350202134	25.25246831355701
48	26.550806758957556	24.746122446978898	21.721404386778637	26.98166640728491
49	28.23760512620046	23.63123021703513	22.53741530777177	25.593749348992638
50	27.91317369305294	24.084760239350132	20.757668407179526	27.244397660417402
51	27.287838998680215	26.65202691524834	21.599964707742117	24.460169378329326
52	28.04043414378899	26.1703121036514	21.480118082012837	24.309135670546773
53	25.686330254655697	26.906486545439396	21.6720197342542	25.735163465650707
54	26.269939206634945	25.0908346741532	22.448817035376813	26.190409083835043
55	26.037292152496867	26.544434545728596	22.544461505092254	24.873811796682283
56	27.09655005924933	24.85077379500835	21.239260676214368	26.81341546952795
57	26.68388798139314	24.404535055138027	21.55425633208054	27.357320631388298
58	27.40125213989949	24.073608866199454	21.696099347705943	26.829039646195113
59	26.30608926245308	24.472484905819915	22.98971990446582	26.231705927261185
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	3.5
7	6.0
8	5.0
9	26.0
10	48.0
11	224.5
12	401.0
13	473.5
14	709.5
15	873.0
16	989.0
17	1105.0
18	1175.5
19	1521.5
20	1797.0
21	2097.5
22	2398.0
23	2989.0
24	3580.0
25	5065.5
26	8396.0
27	10241.0
28	11401.0
29	12561.0
30	13796.5
31	15572.5
32	16113.0
33	16540.5
34	16968.0
35	18799.0
36	23296.5
37	25963.0
38	26667.0
39	27371.0
40	29086.0
41	30801.0
42	33065.0
43	37260.5
44	39192.0
45	42007.5
46	44823.0
47	45714.5
48	54335.5
49	62065.0
50	62560.5
51	63056.0
52	71633.5
53	86512.5
54	92814.0
55	103829.0
56	114844.0
57	125758.0
58	139648.0
59	142624.0
60	131624.5
61	120625.0
62	111126.0
63	101627.0
64	95142.0
65	81524.0
66	74391.0
67	64490.5
68	54590.0
69	50612.0
70	38751.5
71	30869.0
72	30880.5
73	30892.0
74	23593.5
75	12802.5
76	9310.0
77	5872.5
78	2435.0
79	2288.5
80	2142.0
81	1443.5
82	510.5
83	276.0
84	179.5
85	83.0
86	57.0
87	20.0
88	9.0
89	5.5
90	2.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1632086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.068638547851492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.4978001273391	13.416959697869604
2	6.452534844126907	2.0736690025538507
3	2.6192292234204224	1.2626234299533758
4	1.5083023202069605	0.9694545921716565
5	0.9291937815216991	0.7465439508091736
6	0.6243478319344653	0.6019451783693792
7	0.42719690580579406	0.48051308677077054
8	0.2987832457156401	0.38408319836468147
9	0.22628627503841833	0.3272501125738848
>10	1.681291688615035	5.32044419066855
>50	0.31222179549363854	3.67809209678079
>100	1.2347126950996956	49.405733539550624
>500	0.17392477926629285	18.408620096905157
>1k	0.014174486415975409	2.9240678266585025
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCGGACACTGGGGATCACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCT	2576	0.15783481997884916	No Hit
AGCCGACACTGGGATCCACGATGGCCTAGCACGTGGCATACGCGAAGCTGCCAAAGCCT	2234	0.13688004186053923	No Hit
GATGGACACTGGGGTCCCCTCCCTCGGCTCTAGACTTGGGGGAGGGGTTGCCACAGGCC	1681	0.10299702344116671	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.004595346078576742	0.0	0.0	0.0	0.0
12	0.006678569634198198	0.0	0.0	0.0	0.0
13	0.010109761372868831	0.0	0.0	0.0	0.0
14	0.031125810772226464	0.0	0.0	0.0	0.0
15	0.05361237091672865	0.0	0.0	0.0	0.0
16	0.07021688808065261	0.0	0.0	0.0	0.0
17	0.0842480114405736	0.0	0.0	0.0	0.0
18	0.10091379988554525	0.0	0.0	0.0	0.0
19	0.1253610410235735	0.0	0.0	0.0	0.0
20	0.14827650013540952	0.0	0.0	0.0	0.0
21	0.17388789561334392	0.0	0.0	0.0	0.0
22	0.1993767485291829	0.0	0.0	0.0	0.0
23	0.22345636198092503	0.0	0.0	0.0	0.0
24	0.2501706405177178	0.0	0.0	0.0	0.0
25	0.27909068517222746	0.0	0.0	0.0	0.0
26	0.3162210814871275	0.0	0.0	0.0	0.0
27	0.3534127490830753	0.0	0.0	0.0	0.0
28	0.3937905232935029	0.0	0.0	0.0	0.0
29	0.43729313283736276	0.0	0.0	0.0	0.0
30	0.4926823709044744	0.0	0.0	0.0	0.0
31	0.5426184649583417	0.0	0.0	0.0	0.0
32	0.5872852288421075	0.0	0.0	0.0	0.0
33	0.6197590077973832	0.0	0.0	0.0	0.0
34	0.6548674518377096	0.0	0.0	0.0	0.0
35	0.6888730128191775	0.0	0.0	0.0	0.0
36	0.7267386645066498	0.0	0.0	0.0	0.0
37	0.7653395715666944	0.0	0.0	0.0	0.0
38	0.807494212927505	0.0	0.0	0.0	0.0
39	0.8573690357003246	0.0	0.0	0.0	0.0
40	0.9072438584731441	0.0	0.0	0.0	0.0
41	0.954484016160913	0.0	0.0	0.0	0.0
42	1.004297567652685	0.0	0.0	0.0	0.0
43	1.0479839910396878	0.0	0.0	0.0	0.0
44	1.0923443985182153	0.0	0.0	0.0	0.0
45	1.1348053962842644	0.0	0.0	0.0	0.0
46	1.1773889366124088	0.0	0.0	0.0	0.0
47	1.215315859580929	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTATG	50	3.6379788E-12	53.000004	49
TCGTTAG	25	1.4790081E-5	53.000004	44
ACACGTA	80	0.0	53.000004	29
CTCCGTA	25	1.4790081E-5	53.000004	33
TAGACCG	20	3.138635E-4	53.000004	31
ACACCTA	40	1.6025297E-9	53.000004	29
CTATACG	25	1.4790081E-5	53.000004	26
GGCGTAA	25	1.4790081E-5	53.000004	48
GCTATAT	50	3.6379788E-12	53.000004	21
GCTATAA	20	3.138635E-4	53.000004	47
ATTCGGC	25	1.4790081E-5	53.000004	46
ATTCGCG	160	0.0	53.000004	47
CGTCTAG	20	3.138635E-4	53.000004	43
CAATTCG	50	3.6379788E-12	53.000004	50
CCTAATA	80	0.0	53.000004	21
TAACGGC	155	0.0	53.000004	40
CCTAACC	20	3.138635E-4	53.000004	41
GCCATAC	20	3.138635E-4	53.000004	21
ATGTACG	25	1.4790081E-5	53.000004	45
TAGTTAG	25	1.4790081E-5	53.000004	42
>>END_MODULE