FastQCFastQC Report
Fri 17 Jun 2016
SRR1528026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11076971
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGA206.3138263E-446.00000446
TCGACAA139400.043.9375882
GACAATA2479250.043.6139564
TAGGGGC1678900.043.586169
CGAACAA102600.043.534112
TGACAAT831700.043.5221823
GAACAAT479650.043.3962253
CATATGC95350.043.3707437
ATAGGGG7707650.043.3540428
TAGGGGA1804350.043.324419
TTACAAT1465200.043.2843323
TAGGGAT484150.043.277919
CAACAAT702200.043.2715763
AATAGGG11498100.043.2547457
ACAATAG11728950.043.2536775
CAATAGG11650950.043.2485166
AACAATA2780450.043.2139784
CGTACAA169850.043.2104762
CGACAAT485900.043.1646423
CACAATA2763350.043.130984