##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528026_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11076971
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79753968842204	33.0	31.0	34.0	30.0	34.0
2	32.35583400913481	34.0	31.0	34.0	30.0	34.0
3	32.51986928556552	34.0	31.0	34.0	31.0	34.0
4	36.036171982394826	37.0	35.0	37.0	35.0	37.0
5	36.04541593545745	37.0	35.0	37.0	35.0	37.0
6	36.143669420096884	37.0	36.0	37.0	35.0	37.0
7	36.077330616826565	37.0	35.0	37.0	35.0	37.0
8	36.12892829637272	37.0	36.0	37.0	35.0	37.0
9	38.00125846677761	39.0	38.0	39.0	35.0	39.0
10	37.715543987611774	39.0	38.0	39.0	35.0	39.0
11	37.85827939786066	39.0	37.0	39.0	35.0	39.0
12	37.96558987109382	39.0	37.0	39.0	35.0	39.0
13	38.049060253024045	39.0	38.0	39.0	37.0	39.0
14	38.67434743667741	40.0	39.0	40.0	36.0	40.0
15	38.13932626527595	40.0	38.0	40.0	34.0	40.0
16	37.3205253493938	39.0	36.0	40.0	33.0	40.0
17	37.27959457508736	39.0	36.0	40.0	33.0	40.0
18	37.24173359305536	40.0	36.0	40.0	33.0	40.0
19	37.39230625411947	40.0	36.0	40.0	33.0	40.0
20	37.40831685846248	40.0	36.0	40.0	33.0	40.0
21	37.15974150334058	40.0	36.0	40.0	32.0	40.0
22	37.22917862653969	40.0	35.0	40.0	32.0	40.0
23	37.20508052246413	40.0	35.0	40.0	32.0	40.0
24	37.19547067515118	40.0	35.0	40.0	32.0	40.0
25	37.351188063957196	40.0	35.0	40.0	33.0	40.0
26	37.37689464024055	40.0	35.0	40.0	33.0	40.0
27	37.38999957659905	40.0	35.0	40.0	33.0	40.0
28	37.36656356688123	40.0	35.0	40.0	33.0	40.0
29	37.37197912678475	40.0	35.0	40.0	33.0	40.0
30	37.339747932896096	40.0	35.0	40.0	33.0	40.0
31	37.29647752982291	40.0	35.0	40.0	33.0	40.0
32	37.242680602847116	40.0	35.0	40.0	33.0	40.0
33	37.16561919318919	40.0	35.0	40.0	33.0	40.0
34	37.042761870551075	40.0	35.0	40.0	33.0	40.0
35	36.94509473754152	39.0	35.0	40.0	32.0	40.0
36	36.87203749111558	39.0	35.0	40.0	32.0	40.0
37	36.76603874831847	39.0	35.0	40.0	32.0	40.0
38	36.516505369563575	39.0	35.0	40.0	31.0	40.0
39	36.42115692096693	39.0	35.0	40.0	31.0	40.0
40	36.32216099509514	38.0	35.0	40.0	31.0	40.0
41	36.19670937118099	38.0	35.0	40.0	31.0	40.0
42	36.05955247151952	38.0	35.0	40.0	31.0	40.0
43	35.89199556449141	38.0	35.0	40.0	31.0	40.0
44	35.72147241335199	37.0	35.0	40.0	30.0	40.0
45	35.55194068847883	37.0	35.0	40.0	30.0	40.0
46	35.41195223856774	37.0	35.0	40.0	30.0	40.0
47	35.26134996652063	36.0	34.0	40.0	30.0	40.0
48	35.10282522180477	36.0	34.0	40.0	29.0	40.0
49	34.942646595355356	35.0	34.0	40.0	29.0	40.0
50	34.79096081410703	35.0	34.0	39.0	28.0	40.0
51	34.62173819900765	35.0	34.0	39.0	28.0	40.0
52	33.966239778004294	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	12.0
13	26.0
14	36.0
15	59.0
16	167.0
17	542.0
18	1460.0
19	3058.0
20	5640.0
21	9683.0
22	16081.0
23	24290.0
24	35588.0
25	50652.0
26	69043.0
27	92683.0
28	121300.0
29	156138.0
30	199350.0
31	251049.0
32	312412.0
33	403446.0
34	634067.0
35	1315159.0
36	926326.0
37	1762628.0
38	3252202.0
39	1433870.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.84794047036866	19.507733657513413	34.331831328257515	17.31249454386041
2	23.01563306430973	21.07364007723772	35.87292952197853	20.03779733647402
3	22.22780036166927	21.070706062153636	36.037983669001214	20.663509907175886
4	21.33579658193562	23.53758983389954	31.813128336257268	23.313485247907572
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	100.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.5783504353311	0.364648422389117	0.04117551630314822	0.01582562597663206
12	99.26861774757738	0.6409152827067977	0.057389335044751855	0.03307763467106666
13	98.58398112624832	0.8131374542733749	0.2149865698844928	0.38789484959381043
14	66.9052487363197	19.80102683305752	6.384958487297656	6.908765943325121
15	40.18702405197233	25.44022188015117	14.786993664603798	19.5857604032727
16	34.75441977775332	15.803878154054932	23.96944074332234	25.472261324869404
17	25.896113657786053	15.475792073482905	26.48630207662365	32.141792192107395
18	23.75829096239396	17.96455005614802	31.379851044116663	26.897307937341353
19	28.580249961835236	16.842681993118877	28.488311470708013	26.088756574337875
20	31.64475198138553	17.259276024104423	25.04429234309632	26.05167965141373
21	33.156401691401015	17.90326073797611	22.550532993180177	26.389804577442693
22	32.225109192756754	19.570530608051605	22.30118684972634	25.903173349465302
23	30.47303274514305	21.850919353314186	20.568465873928893	27.10758202761387
24	30.446762025467073	24.709516708132576	19.61829637362055	25.225424892779802
25	30.210343603860657	21.640672346257837	22.98783665679002	25.161147393091486
26	29.052247225347074	20.72943948305001	23.042978084893427	27.175335206709487
27	31.48425684241658	20.898709584055062	21.492482015164615	26.124551558363745
28	33.05357574737715	20.054814623961732	20.010362038503125	26.881247590157997
29	32.13039015810369	19.07015013400324	18.643571423993073	30.155888283899994
30	29.20774099706499	18.149736060516904	21.382117909309322	31.26040503310878
31	27.09604457752936	18.390478768970326	21.651180634128227	32.86229601937208
32	28.602819308635908	23.640135918023077	18.665978271496783	29.09106650184423
33	32.23566261932075	22.576939128936964	18.73967170267034	26.44772654907194
34	31.09440297352047	20.63435031110942	21.753266303577036	26.51798041179308
35	33.07770689297643	21.263917726244838	20.582684562413316	25.07569081836542
36	36.20034754988525	20.036343870540062	19.798101845712154	23.965206733862534
37	35.58090022985525	19.875307067247896	19.7849845413516	24.758808161545247
38	30.883126804250004	23.262848661425583	23.330746284340727	22.523278249983683
39	29.809051590005968	25.777651670298678	23.55439948339668	20.858897256298675
40	30.708322699409436	28.232636882411267	18.924893817994107	22.134146600185193
41	31.44151952731482	26.377644213386493	18.180999119705195	23.999837139593485
42	32.37604395642094	25.596591342524956	17.345174958027787	24.68218974302632
43	32.1768469015582	24.18734327281348	17.384066456434706	26.25174336919362
44	31.199151825891754	23.117529151245407	18.877669716748375	26.80564930611446
45	27.83993927581827	23.80612894987267	20.282629610567724	28.071302163741336
46	27.79390683608362	23.123180515684297	19.747257621239598	29.335655026992484
47	28.449465110994694	19.275783966573535	22.134597987121207	30.140152935310567
48	29.975685591304696	18.638913110813416	20.63778987956184	30.74761141832004
49	30.679912405656744	20.168564131837126	20.980843950932073	28.170679511574058
50	29.072794358674408	22.909105747410553	19.96232544077257	28.05577445314247
51	27.68394897847074	22.405764175061936	22.069345491651102	27.840941354816223
52	28.942424783814996	21.496788246534184	22.98407208974367	26.576714879907154
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	2.5
2	5.0
3	29.0
4	53.0
5	107.0
6	161.0
7	324.5
8	488.0
9	891.0
10	1294.0
11	1931.5
12	2569.0
13	3431.0
14	5509.0
15	6725.0
16	7638.5
17	8552.0
18	11281.5
19	14011.0
20	18633.5
21	23256.0
22	32186.5
23	41117.0
24	51084.5
25	61052.0
26	64303.5
27	67555.0
28	68557.0
29	69559.0
30	71614.0
31	73669.0
32	82031.0
33	90393.0
34	100067.5
35	109742.0
36	117063.0
37	124384.0
38	149420.0
39	191122.0
40	207788.0
41	246017.5
42	284247.0
43	335722.5
44	387198.0
45	419958.0
46	452718.0
47	492047.5
48	531377.0
49	556900.0
50	582423.0
51	641159.5
52	699896.0
53	804931.0
54	909966.0
55	986566.0
56	1063166.0
57	1103479.0
58	1143792.0
59	1081689.5
60	1019587.0
61	938994.5
62	858402.0
63	742253.0
64	555012.0
65	483920.0
66	423983.5
67	364047.0
68	308737.5
69	253428.0
70	211801.0
71	170174.0
72	129832.5
73	89491.0
74	68396.0
75	47301.0
76	33179.0
77	19057.0
78	12507.5
79	5958.0
80	3997.5
81	2037.0
82	1521.0
83	1005.0
84	702.0
85	399.0
86	264.0
87	129.0
88	78.0
89	13.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.1076971E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.937037673582544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.96699514772897	4.923007189207454
2	8.752437324955135	1.214319749177676
3	4.407124243345933	0.9171716072474911
4	2.6717827407257304	0.7413703011216806
5	1.7487703636123242	0.6065642947411669
6	1.213544501017493	0.5051042353276367
7	0.8009507346925028	0.3889357794871856
8	0.5808557126737208	0.32235343693865914
9	0.4531627707228957	0.28292464914927984
>10	3.0274324608203247	4.424183334964755
>50	1.132459587455345	5.8847093625637825
>100	3.865501396007985	61.11450855680985
>500	0.34309748255889144	15.407156357540003
>1k	0.035885533682890006	3.2676911457234024
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.020393661769088318	0.0	0.0	0.0	0.0
11	0.021910321874093558	0.0	0.0	0.0	0.0
12	0.029222790237511682	0.0	0.0	0.0	0.0
13	0.035749845332266376	0.0	0.0	0.0	0.0
14	0.053940738853608984	0.0	0.0	0.0	0.0
15	0.07788230193976313	0.0	0.0	0.0	0.0
16	0.08954614036635106	0.0	0.0	0.0	0.0
17	0.09860096230278115	0.0	0.0	0.0	0.0
18	0.11548283370968472	0.0	0.0	0.0	0.0
19	0.12920499656449402	0.0	0.0	0.0	0.0
20	0.14141952705301838	0.0	0.0	0.0	0.0
21	0.15609863021217624	0.0	0.0	0.0	0.0
22	0.1715992575948786	0.0	0.0	0.0	0.0
23	0.18877904437955106	0.0	0.0	0.0	9.027738720269287E-6
24	0.20656368965848154	0.0	0.0	0.0	9.027738720269287E-6
25	0.22392403121755938	0.0	0.0	0.0	9.027738720269287E-6
26	0.24493157921962602	0.0	0.0	0.0	9.027738720269287E-6
27	0.2685120327569694	0.0	0.0	0.0	9.027738720269287E-6
28	0.29016055020817516	0.0	0.0	0.0	9.027738720269287E-6
29	0.3131090620350997	0.0	0.0	0.0	9.027738720269287E-6
30	0.34039088844775345	0.0	0.0	0.0	9.027738720269287E-6
31	0.366860218375583	0.0	0.0	0.0	9.027738720269287E-6
32	0.3977170293214634	0.0	0.0	0.0	9.027738720269287E-6
33	0.4218301194433027	0.0	0.0	0.0	9.027738720269287E-6
34	0.4415015621147695	0.0	0.0	0.0	9.027738720269287E-6
35	0.4602160644818877	0.0	0.0	0.0	9.027738720269287E-6
36	0.47851529086787353	0.0	0.0	0.0	9.027738720269287E-6
37	0.4985749263043119	0.0	0.0	0.0	9.027738720269287E-6
38	0.5223088513998998	0.0	0.0	0.0	9.027738720269287E-6
39	0.5525698315902425	0.0	0.0	0.0	9.027738720269287E-6
40	0.5986203268023361	0.0	0.0	0.0	9.027738720269287E-6
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCGA	20	6.3138263E-4	46.000004	46
TCGACAA	13940	0.0	43.937588	2
GACAATA	247925	0.0	43.613956	4
TAGGGGC	167890	0.0	43.58616	9
CGAACAA	10260	0.0	43.53411	2
TGACAAT	83170	0.0	43.522182	3
GAACAAT	47965	0.0	43.396225	3
CATATGC	9535	0.0	43.37074	37
ATAGGGG	770765	0.0	43.354042	8
TAGGGGA	180435	0.0	43.32441	9
TTACAAT	146520	0.0	43.284332	3
TAGGGAT	48415	0.0	43.27791	9
CAACAAT	70220	0.0	43.271576	3
AATAGGG	1149810	0.0	43.254745	7
ACAATAG	1172895	0.0	43.253677	5
CAATAGG	1165095	0.0	43.248516	6
AACAATA	278045	0.0	43.213978	4
CGTACAA	16985	0.0	43.210476	2
CGACAAT	48590	0.0	43.164642	3
CACAATA	276335	0.0	43.13098	4
>>END_MODULE