FastQCFastQC Report
Fri 17 Jun 2016
SRR1528025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10987753
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAATA2480600.043.6857224
TAGGGGC1648200.043.654239
TGACAAT852850.043.651053
TCGACAA139200.043.4554632
TTACAAT1455650.043.4513783
TTAACAA362300.043.4352722
ATAGGGG7648950.043.425758
CAACAAT686550.043.4003333
TAGGGAT478400.043.3605779
CGAACAA103200.043.347872
AACAATA2752800.043.3472484
ACAATAG11612150.043.340145
AATAGGG11381000.043.3388637
GAACAAT493850.043.3360333
CAATAGG11538000.043.334616
CGTACAA165650.043.306372
ATAGGGC761700.043.303538
ATTACAA314550.043.2799262
TTGACAA315700.043.2752572
CATATGC87700.043.27252237
Produced by FastQC (version 0.11.5)