##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528025_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10987753
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73903472347804	31.0	31.0	34.0	30.0	34.0
2	32.192187156009055	34.0	31.0	34.0	30.0	34.0
3	32.44953222009996	34.0	31.0	34.0	30.0	34.0
4	36.005057539972	37.0	35.0	37.0	35.0	37.0
5	36.02931500189347	37.0	35.0	37.0	35.0	37.0
6	36.130881991977795	37.0	36.0	37.0	35.0	37.0
7	36.07711949840882	37.0	35.0	37.0	35.0	37.0
8	36.072975976070815	37.0	35.0	37.0	35.0	37.0
9	37.990718120438274	39.0	38.0	39.0	35.0	39.0
10	37.70133220140642	39.0	38.0	39.0	35.0	39.0
11	37.841400238975154	39.0	37.0	39.0	35.0	39.0
12	37.929878656718984	39.0	37.0	39.0	35.0	39.0
13	38.057694234662904	39.0	39.0	39.0	37.0	39.0
14	38.70461576629908	40.0	39.0	40.0	36.0	40.0
15	38.16352997742123	40.0	38.0	40.0	34.0	40.0
16	37.352745643263006	39.0	36.0	40.0	33.0	40.0
17	37.322472301661676	40.0	36.0	40.0	33.0	40.0
18	37.3092683281104	40.0	36.0	40.0	33.0	40.0
19	37.45004597391296	40.0	36.0	40.0	33.0	40.0
20	37.44497041387807	40.0	36.0	40.0	33.0	40.0
21	37.39076551866428	40.0	36.0	40.0	33.0	40.0
22	37.3570912087303	40.0	36.0	40.0	32.0	40.0
23	37.30458866339642	40.0	35.0	40.0	32.0	40.0
24	37.30016464694829	40.0	35.0	40.0	32.0	40.0
25	37.2676504468202	40.0	35.0	40.0	32.0	40.0
26	37.290670713111226	40.0	35.0	40.0	33.0	40.0
27	37.34992395624474	40.0	35.0	40.0	33.0	40.0
28	37.1855953624003	40.0	35.0	40.0	33.0	40.0
29	37.295627322529	40.0	35.0	40.0	33.0	40.0
30	37.32678865278461	40.0	35.0	40.0	33.0	40.0
31	37.32294036824454	40.0	35.0	40.0	33.0	40.0
32	37.266547946609286	40.0	35.0	40.0	33.0	40.0
33	37.18282673445608	40.0	35.0	40.0	33.0	40.0
34	37.08473852661231	40.0	35.0	40.0	33.0	40.0
35	36.92039655423634	39.0	35.0	40.0	32.0	40.0
36	36.471003216035164	39.0	35.0	40.0	31.0	40.0
37	36.58667217947109	39.0	35.0	40.0	31.0	40.0
38	36.45312221707204	39.0	35.0	40.0	31.0	40.0
39	36.39244129350195	39.0	35.0	40.0	31.0	40.0
40	36.302908884100326	38.0	35.0	40.0	31.0	40.0
41	36.21108719862924	38.0	35.0	40.0	31.0	40.0
42	35.92930638320683	38.0	35.0	40.0	31.0	40.0
43	35.76256128072773	38.0	35.0	40.0	30.0	40.0
44	35.59797694760703	37.0	35.0	40.0	30.0	40.0
45	35.18825323066509	37.0	34.0	40.0	28.0	40.0
46	35.20510153440836	37.0	34.0	40.0	29.0	40.0
47	35.135849431635386	36.0	34.0	40.0	29.0	40.0
48	34.97657510138788	36.0	34.0	40.0	29.0	40.0
49	34.819650478127784	35.0	34.0	40.0	28.0	40.0
50	34.60365399549844	35.0	34.0	39.0	27.0	40.0
51	34.52416212850799	35.0	34.0	39.0	27.0	40.0
52	34.01576819209533	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	9.0
13	17.0
14	17.0
15	81.0
16	207.0
17	583.0
18	1473.0
19	3150.0
20	5708.0
21	9760.0
22	16157.0
23	24732.0
24	36286.0
25	51315.0
26	70658.0
27	93464.0
28	123583.0
29	159494.0
30	203959.0
31	254315.0
32	317216.0
33	413360.0
34	646197.0
35	1291143.0
36	939134.0
37	1771636.0
38	3139930.0
39	1414165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.8730917049191	19.501403062118342	34.29502829195378	17.330476941008776
2	23.132463935073897	21.13173639778761	35.740719690367996	19.995079976770498
3	22.27086830219063	20.998579054334403	36.07249817137316	20.658054472101803
4	21.336145797962512	23.538770847870353	31.817852112256258	23.30723124191088
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	100.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.57213271903727	0.3660530046498133	0.044176457188289546	0.017637819124619928
12	99.2715708116118	0.6388476333605242	0.059748339810696514	0.029833215216978393
13	98.58784594083977	0.8104477776302398	0.21311909723489417	0.38858718429509653
14	66.90702821586908	19.81998503242656	6.380831458442867	6.892155293261506
15	40.18085408363293	25.46523388357929	14.810079913518258	19.543832119269517
16	34.77632778967638	15.803030883566457	23.963261642303028	25.45737968445414
17	25.896541358365084	15.483320384067605	26.506565992155085	32.11357226541223
18	23.771803024694858	17.97815258497347	31.346072304319183	26.90397208601249
19	28.57718042988407	16.865140670708563	28.500112807413853	26.05756609199351
20	31.652904829586177	17.265923251096016	25.047523365332292	26.03364855398552
21	32.8954336705603	17.98154727358724	22.56399465841651	26.559024397435945
22	32.24697533699565	19.593724030745868	22.309010768625757	25.85028986363272
23	30.47536652853409	21.85627034025974	20.56596102952078	27.102402101685396
24	30.428323243159905	24.728813980438037	19.623666458465166	25.219196317936888
25	30.253210096732243	21.63601602620663	22.952618246879048	25.158155630182076
26	29.051244599327998	20.731308758032693	23.0374763611814	27.179970281457912
27	31.47810111858175	20.92113828914793	21.46447958922994	26.136281003040384
28	33.15842647718783	20.069763126273408	20.00754840411866	26.764261992420106
29	32.11757672383062	19.109930847553635	18.63587577915157	30.13661664946418
30	29.20312278588716	18.177101360032392	21.373887818555804	31.245888035524644
31	27.09209062125805	18.40143294083877	21.63980205962038	32.8666743782828
32	28.585662600897564	23.661525700477615	18.673426677865802	29.07938502075902
33	32.2548750413301	22.5920531704708	18.718285713193588	26.434786075005505
34	31.08473588731017	20.65825469502272	21.746657392098275	26.510352025568828
35	33.1058679604465	21.307923467154747	20.563749476348804	25.022459096049936
36	36.2329313372807	19.991958319412532	19.870859856423785	23.904250486882987
37	35.57776553586525	19.874017917949192	19.78333513685646	24.764881409329096
38	30.87600349225178	23.30472845539939	23.338729947788234	22.480538104560598
39	29.814776506170098	25.786819197701295	23.5478081824373	20.850596113691306
40	30.730614348538776	28.253137834459874	18.898486342020977	22.117761474980373
41	31.434725553077143	26.386423138561632	18.188250136310856	23.990601172050372
42	32.4447136734872	25.579770495387	17.404468411330324	24.571047419795473
43	32.123556108332615	24.223405822828383	17.398752957042262	26.254285111796744
44	31.41319248803645	23.062304003375395	18.820535918490343	26.70396759009781
45	27.88411789016371	23.80067152947468	20.340455414314466	27.974755166047142
46	27.802663565516987	23.217658787925068	19.76048242074608	29.219195225811866
47	28.44477847290524	19.291960785794878	22.12386827406841	30.139392467231474
48	30.024127772074966	18.64452176891854	20.63926082066097	30.692089638345525
49	30.735774639273377	20.131559200502597	20.974415788196186	28.158250372027837
50	29.20423311299408	22.859896832409685	19.97655935658546	27.959310698010775
51	27.674411683626303	22.43544244214445	22.05939649353239	27.830749380696858
52	28.956065903556443	21.495150100298034	22.92588848693632	26.62289550920921
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	2.0
2	4.0
3	30.5
4	57.0
5	88.5
6	120.0
7	305.5
8	491.0
9	838.0
10	1185.0
11	1903.5
12	2622.0
13	3408.0
14	5400.5
15	6607.0
16	7523.0
17	8439.0
18	11080.0
19	13721.0
20	18425.5
21	23130.0
22	31549.0
23	39968.0
24	49757.0
25	59546.0
26	63365.5
27	67185.0
28	68227.5
29	69270.0
30	71173.0
31	73076.0
32	81417.5
33	89759.0
34	99399.0
35	109039.0
36	117018.5
37	124998.0
38	148506.5
39	189524.0
40	207033.0
41	244589.0
42	282145.0
43	333268.5
44	384392.0
45	417176.0
46	449960.0
47	488729.5
48	527499.0
49	553338.0
50	579177.0
51	638570.0
52	697963.0
53	801523.0
54	905083.0
55	979335.5
56	1053588.0
57	1092224.5
58	1130861.0
59	1070472.0
60	1010083.0
61	929834.5
62	849586.0
63	735566.5
64	550521.5
65	479496.0
66	420118.0
67	360740.0
68	306213.5
69	251687.0
70	209897.5
71	168108.0
72	128072.5
73	88037.0
74	67577.0
75	47117.0
76	32884.0
77	18651.0
78	12284.5
79	5918.0
80	3986.0
81	2054.0
82	1531.0
83	1008.0
84	719.5
85	431.0
86	291.5
87	152.0
88	81.5
89	5.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.0987753E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.168220438027477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.73235523991949	4.998568940225833
2	9.579525325653265	1.3733629845189912
3	4.646747021128087	0.9992672090158097
4	2.7722644739129434	0.7948881144608101
5	1.7460427560826792	0.6258009684910842
6	1.2268300005269104	0.5276512730257356
7	0.8358091173409743	0.41938847980492944
8	0.6021459806975998	0.3453052100410107
9	0.43152211819628955	0.27839211004039965
>10	3.0554069883606907	4.658543562585482
>50	1.1823185979691626	6.3469120526756875
>100	3.853696739384465	61.724632792607345
>500	0.3043528177807619	14.023932872639389
>1k	0.03098282304708111	2.8833534298676695
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.020286222305870908	0.0	0.0	0.0	0.0
11	0.021796995254625764	0.0	0.0	0.0	0.0
12	0.029560183961179324	0.0	0.0	0.0	0.0
13	0.03590361013757772	0.0	0.0	0.0	0.0
14	0.05415119906681557	0.0	0.0	0.0	0.0
15	0.0781324443678339	0.0	0.0	0.0	0.0
16	0.08912650293467646	0.0	0.0	0.0	0.0
17	0.09831855521324515	0.0	0.0	0.0	0.0
18	0.11485514827280882	0.0	0.0	0.0	0.0
19	0.12774222354652492	0.0	0.0	0.0	0.0
20	0.14052008631792143	0.0	0.0	0.0	0.0
21	0.15472681266133304	0.0	0.0	0.0	0.0
22	0.1699801588186411	0.0	0.0	0.0	0.0
23	0.18704461230608296	0.0	0.0	0.0	0.0
24	0.20566534395157954	0.0	0.0	0.0	0.0
25	0.22330316307619946	0.0	0.0	0.0	0.0
26	0.2433527582937112	0.0	0.0	0.0	0.0
27	0.2663692931575728	0.0	0.0	0.0	0.0
28	0.2889489780121559	0.0	0.0	0.0	0.0
29	0.31145585453185926	0.0	0.0	0.0	0.0
30	0.3387134749024664	0.0	0.0	0.0	0.0
31	0.36665367341257127	0.0	0.0	0.0	0.0
32	0.39690553655510824	0.0	0.0	0.0	0.0
33	0.42085947873054663	0.0	0.0	0.0	0.0
34	0.4393891999574435	0.0	0.0	0.0	0.0
35	0.45759128367738156	0.0	0.0	0.0	9.101041859969004E-6
36	0.4757478621880197	0.0	0.0	0.0	1.8202083719938008E-5
37	0.49508757614045384	0.0	0.0	0.0	1.8202083719938008E-5
38	0.5192144381112317	0.0	0.0	0.0	1.8202083719938008E-5
39	0.5488656324910107	0.0	0.0	0.0	1.8202083719938008E-5
40	0.5930875948886001	0.0	0.0	0.0	1.8202083719938008E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACAATA	248060	0.0	43.685722	4
TAGGGGC	164820	0.0	43.65423	9
TGACAAT	85285	0.0	43.65105	3
TCGACAA	13920	0.0	43.455463	2
TTACAAT	145565	0.0	43.451378	3
TTAACAA	36230	0.0	43.435272	2
ATAGGGG	764895	0.0	43.42575	8
CAACAAT	68655	0.0	43.400333	3
TAGGGAT	47840	0.0	43.360577	9
CGAACAA	10320	0.0	43.34787	2
AACAATA	275280	0.0	43.347248	4
ACAATAG	1161215	0.0	43.34014	5
AATAGGG	1138100	0.0	43.338863	7
GAACAAT	49385	0.0	43.336033	3
CAATAGG	1153800	0.0	43.33461	6
CGTACAA	16565	0.0	43.30637	2
ATAGGGC	76170	0.0	43.30353	8
ATTACAA	31455	0.0	43.279926	2
TTGACAA	31570	0.0	43.275257	2
CATATGC	8770	0.0	43.272522	37
>>END_MODULE